{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"F4K265","secondaryAccessions":["Q84WU3","Q93ZX0","Q9FKY8"],"uniProtkbId":"AT101_ARATH","entryAudit":{"firstPublicDate":"2015-11-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-06-28","entryVersion":86,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24563201"}],"value":"Autophagy-related protein 101"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24563201"}],"value":"ATG101"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT5G66930"}],"value":"At5g66930"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAB08635.1"}],"value":"MUD21.19"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9SUG7"}],"value":"Accessory protein involved in autophagy. Acts as a scaffold protein of the ATG1-ATG13 complex for faithful delivery of autophagic vesicles to the vacuole. Required for selective mitophagy"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24563201"}],"value":"Interacts with ATG11 and ATG13A"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24563201"}],"value":"Cytoplasmic vesicle, autophagosome","id":"SL-0023"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["F4K265-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["F4K265-2"],"sequenceIds":["VSP_057968","VSP_057969"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ATG101 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"BAB08635.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"BX833047","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"Autophagy-related protein 101","featureId":"PRO_0000434629"},{"type":"Region","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Alternative sequence","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"in isoform 2","featureId":"VSP_057968","alternativeSequence":{"originalSequence":"EASEERSSRRTLLEQSLQE","alternativeSequences":["GSIDQFPVLISKNYIFLLL"]}},{"type":"Alternative sequence","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":215,"modifier":"EXACT"}},"description":"in isoform 2","featureId":"VSP_057969","alternativeSequence":{}},{"type":"Sequence conflict","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"in Ref. 4; AAL07060","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["E"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"9679202","citationType":"journal article","authors":["Kaneko T.","Kotani H.","Nakamura Y.","Sato S.","Asamizu E.","Miyajima N.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"9679202"},{"database":"DOI","id":"10.1093/dnares/5.2.131"}],"title":"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones.","publicationDate":"1998","journal":"DNA Res.","firstPage":"131","lastPage":"145","volume":"5"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"14993207","citationType":"journal article","authors":["Castelli V.","Aury J.-M.","Jaillon O.","Wincker P.","Clepet C.","Menard M.","Cruaud C.","Quetier F.","Scarpelli C.","Schaechter V.","Temple G.","Caboche M.","Weissenbach J.","Salanoubat M."],"citationCrossReferences":[{"database":"PubMed","id":"14993207"},{"database":"DOI","id":"10.1101/gr.1515604"}],"title":"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation.","publicationDate":"2004","journal":"Genome Res.","firstPage":"406","lastPage":"413","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"24563201","citationType":"journal article","authors":["Li F.","Chung T.","Vierstra R.D."],"citationCrossReferences":[{"database":"PubMed","id":"24563201"},{"database":"DOI","id":"10.1105/tpc.113.120014"}],"title":"AUTOPHAGY-RELATED11 plays a critical role in general autophagy- and senescence-induced mitophagy in Arabidopsis.","publicationDate":"2014","journal":"Plant Cell","firstPage":"788","lastPage":"807","volume":"26"},"referencePositions":["INTERACTION WITH ATG11 AND ATG13A","SUBCELLULAR LOCATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB010700","properties":[{"key":"ProteinId","value":"BAB08635.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED98279.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED98280.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408687","properties":[{"key":"ProteinId","value":"CAO7058095.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY056211","properties":[{"key":"ProteinId","value":"AAL07060.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT002759","properties":[{"key":"ProteinId","value":"AAO22587.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BX833047","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_569042.2","properties":[{"key":"NucleotideSequenceId","value":"NM_126092.5"}],"isoformId":"F4K265-1"},{"database":"RefSeq","id":"NP_851284.1","properties":[{"key":"NucleotideSequenceId","value":"NM_180953.2"}],"isoformId":"F4K265-2"},{"database":"AlphaFoldDB","id":"F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"F4K265","properties":[{"key":"Number of interactors","value":"2354"}]},{"database":"IntAct","id":"F4K265","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"3702.F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"246522","properties":[{"key":"Description","value":"-"}],"isoformId":"F4K265-1"},{"database":"GeneID","id":"836827","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT5G66930","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT5G66930","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT5G66930","properties":[{"key":"GeneName","value":"ATG101"}]},{"database":"eggNOG","id":"KOG4493","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_069661_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"VCWEIWT","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10259639at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:F4K265","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 5"}]},{"database":"ExpressionAtlas","id":"F4K265","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:1990316","properties":[{"key":"GoTerm","value":"C:Atg1/ULK1 kinase complex"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005776","properties":[{"key":"GoTerm","value":"C:autophagosome"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0000407","properties":[{"key":"GoTerm","value":"C:phagophore assembly site"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019901","properties":[{"key":"GoTerm","value":"F:protein kinase binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0000045","properties":[{"key":"GoTerm","value":"P:autophagosome assembly"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"InterPro","id":"IPR012445","properties":[{"key":"EntryName","value":"ATG101"}]},{"database":"PANTHER","id":"PTHR13292","properties":[{"key":"EntryName","value":"AUTOPHAGY-RELATED PROTEIN 101"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13292:SF0","properties":[{"key":"EntryName","value":"AUTOPHAGY-RELATED PROTEIN 101"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07855","properties":[{"key":"EntryName","value":"ATG101"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNCEVCQLKELEVESFEIREVLRCILHTIVFHRALGLIRPKDIDLELFEITYVQCGEIEVEKKIDEKIEQFINWIEKHPNKKSQICLSFYEVKSKQPSWFTKIERLYWEQWYINLNVLQPTKPPVGKSHHSKLVMDPGEASEERSSRRTLLEQSLQEVLFQIIKFVNEKKDHVPPINDGVIYYPFEITIPSSSDSAFGMDMFKRILHSGHPSMLG","length":215,"molWeight":25278,"crc64":"9721238C4715AE7D","md5":"9EC0C9A1DEF282D4CA643A4BDEA19FFC"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"SIMILARITY":1,"SEQUENCE CAUTION":2},"countByFeatureType":{"Chain":1,"Region":1,"Alternative sequence":2,"Sequence conflict":1},"uniParcId":"UPI000019717A"}}