{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"F4IME1","uniProtkbId":"MSL7_ARATH","entryAudit":{"firstPublicDate":"2012-01-25","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-06-28","entryVersion":73,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Mechanosensitive ion channel protein 7"}},"alternativeNames":[{"fullName":{"value":"Mechanosensitive channel of small conductance-like 7"}},{"fullName":{"value":"MscS-Like protein 7"}}]},"genes":[{"geneName":{"value":"MSL7"},"orderedLocusNames":[{"value":"At2g17000"}],"orfNames":[{"value":"F6P23.16"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Membrane","id":"SL-0162"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26973252"}],"value":"Expressed during somatic embryogenesis"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the MscS (TC 1.A.23) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":849,"modifier":"EXACT"}},"description":"Mechanosensitive ion channel protein 7","featureId":"PRO_0000415329"},{"type":"Transmembrane","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":251,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":274,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":313,"modifier":"EXACT"},"end":{"value":333,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":344,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":606,"modifier":"EXACT"},"end":{"value":626,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":642,"modifier":"EXACT"},"end":{"value":662,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":9,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0407","category":"Molecular function","name":"Ion channel"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"10617197","citationType":"journal article","authors":["Lin X.","Kaul S.","Rounsley S.D.","Shea T.P.","Benito M.-I.","Town C.D.","Fujii C.Y.","Mason T.M.","Bowman C.L.","Barnstead M.E.","Feldblyum T.V.","Buell C.R.","Ketchum K.A.","Lee J.J.","Ronning C.M.","Koo H.L.","Moffat K.S.","Cronin L.A.","Shen M.","Pai G.","Van Aken S.","Umayam L.","Tallon L.J.","Gill J.E.","Adams M.D.","Carrera A.J.","Creasy T.H.","Goodman H.M.","Somerville C.R.","Copenhaver G.P.","Preuss D.","Nierman W.C.","White O.","Eisen J.A.","Salzberg S.L.","Fraser C.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10617197"},{"database":"DOI","id":"10.1038/45471"}],"title":"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.","publicationDate":"1999","journal":"Nature","firstPage":"761","lastPage":"768","volume":"402"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"12626684","citationType":"journal article","authors":["Pivetti C.D.","Yen M.R.","Miller S.","Busch W.","Tseng Y.H.","Booth I.R.","Saier M.H. Jr."],"citationCrossReferences":[{"database":"PubMed","id":"12626684"},{"database":"DOI","id":"10.1128/mmbr.67.1.66-85.2003"}],"title":"Two families of mechanosensitive channel proteins.","publicationDate":"2003","journal":"Microbiol. Mol. Biol. Rev.","firstPage":"66","lastPage":"85","volume":"67"},"referencePositions":["GENE FAMILY"]},{"referenceNumber":5,"citation":{"id":"CI-BRA5MCK40IR1O","citationType":"book","authors":["Haswell E.S."],"citationCrossReferences":[{"database":"DOI","id":"10.1016/S1063-5823(06)58013-5"}],"title":"MscS-like proteins in plants.","publicationDate":"2007","bookName":"Mechanosensitive Ion Channels, Part A","editors":["Hamill O.P."],"firstPage":"329","lastPage":"349","publisher":"Academic Press","address":"San Diego."},"referencePositions":["REVIEW","GENE FAMILY","NOMENCLATURE"]},{"referenceNumber":6,"citation":{"id":"26973252","citationType":"journal article","authors":["Nowak K.","Gaj M.D."],"citationCrossReferences":[{"database":"PubMed","id":"26973252"},{"database":"DOI","id":"10.1016/j.jplph.2016.02.012"}],"title":"Stress-related function of bHLH109 in somatic embryo induction in Arabidopsis.","publicationDate":"2016","journal":"J. Plant Physiol.","firstPage":"119","lastPage":"126","volume":"193"},"referencePositions":["DEVELOPMENTAL STAGE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP002685","properties":[{"key":"ProteinId","value":"AEC06571.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408684","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_179292.1","properties":[{"key":"NucleotideSequenceId","value":"NM_127253.1"}]},{"database":"AlphaFoldDB","id":"F4IME1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"F4IME1","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"F4IME1","properties":[{"key":"Number of interactors","value":"14"}]},{"database":"STRING","id":"3702.F4IME1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-F4IME1","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"816203","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT2G17000","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT2G17000","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT2G17000","properties":[{"key":"GeneName","value":"-"}]},{"database":"eggNOG","id":"KOG4629","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_013552_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"F4IME1","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:F4IME1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 2"}]},{"database":"ExpressionAtlas","id":"F4IME1","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008381","properties":[{"key":"GoTerm","value":"F:mechanosensitive monoatomic ion channel activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-ARBA"}]},{"database":"FunFam","id":"2.30.30.60:FF:000003","properties":[{"key":"EntryName","value":"Predicted mechanosensitive ion channel"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.30.60","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010920","properties":[{"key":"EntryName","value":"LSM_dom_sf"}]},{"database":"InterPro","id":"IPR016688","properties":[{"key":"EntryName","value":"MscS-like_plants/fungi"}]},{"database":"InterPro","id":"IPR023408","properties":[{"key":"EntryName","value":"MscS_beta-dom_sf"}]},{"database":"InterPro","id":"IPR006685","properties":[{"key":"EntryName","value":"MscS_channel_2nd"}]},{"database":"PANTHER","id":"PTHR31618","properties":[{"key":"EntryName","value":"MECHANOSENSITIVE ION CHANNEL PROTEIN 5"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR31618:SF18","properties":[{"key":"EntryName","value":"MECHANOSENSITIVE ION CHANNEL PROTEIN 7"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00924","properties":[{"key":"EntryName","value":"MS_channel_2nd"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF017209","properties":[{"key":"EntryName","value":"Memb_At2g17000_prd"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50182","properties":[{"key":"EntryName","value":"Sm-like ribonucleoproteins"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEFRKPFKSHSSYKQIISTGDQNEKTKKKKKLANLDDGDIAKTQSSGSSFDGNSYKFWQDIATDDYTKSGSFDFPQYREEITLDVNEETEETEDVSNNNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSSTSFSSATMRLNLEQQLEDEGEVVVRCSSVRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTMNMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAWSQNPDLRRIILLEC","length":849,"molWeight":97372,"crc64":"7E3949CB13577924","md5":"619BA55803F83597142401A6C843F72A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"DEVELOPMENTAL STAGE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":6,"Region":1,"Compositional bias":1},"uniParcId":"UPI000016233A"}}