{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"F4HYJ7","secondaryAccessions":["Q9C734"],"uniProtkbId":"DEXH3_ARATH","entryAudit":{"firstPublicDate":"2016-01-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2011-06-28","entryVersion":95,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"DExH-box ATP-dependent RNA helicase DExH3"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"3.6.4.13"}]}},"genes":[{"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT1G48650"}],"value":"At1g48650"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAG60124.1"}],"value":"F11I4_16"}]}],"comments":[{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O = ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13065"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"3.6.4.13","evidences":[{"evidenceCode":"ECO:0000305"}]}},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["F4HYJ7-1"],"isoformSequenceStatus":"Displayed"}],"note":{"texts":[{"value":"A number of isoforms are produced. According to EST sequences."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DExH box helicase family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAG60124.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1197,"modifier":"EXACT"}},"description":"DExH-box ATP-dependent RNA helicase DExH3","featureId":"PRO_0000435293"},{"type":"Domain","location":{"start":{"value":309,"modifier":"EXACT"},"end":{"value":476,"modifier":"EXACT"}},"description":"Helicase ATP-binding","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00541"}]},{"type":"Domain","location":{"start":{"value":564,"modifier":"EXACT"},"end":{"value":738,"modifier":"EXACT"}},"description":"Helicase C-terminal","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00542"}]},{"type":"Motif","location":{"start":{"value":423,"modifier":"EXACT"},"end":{"value":426,"modifier":"EXACT"}},"description":"DEIH box","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Binding site","location":{"start":{"value":322,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00541"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0347","category":"Molecular function","name":"Helicase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"24265739","citationType":"journal article","authors":["Xu R.","Zhang S.","Huang J.","Zheng C."],"citationCrossReferences":[{"database":"PubMed","id":"24265739"},{"database":"DOI","id":"10.1371/journal.pone.0078982"}],"title":"Genome-wide comparative in silico analysis of the RNA helicase gene family in Zea mays and Glycine max: a comparison with Arabidopsis and Oryza sativa.","publicationDate":"2013","journal":"PLoS ONE","firstPage":"E78982","lastPage":"E78982","volume":"8"},"referencePositions":["GENE FAMILY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC073555","properties":[{"key":"ProteinId","value":"AAG60124.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE32333.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7030263.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_175298.2","properties":[{"key":"NucleotideSequenceId","value":"NM_103761.6"}],"isoformId":"F4HYJ7-1"},{"database":"AlphaFoldDB","id":"F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"F4HYJ7","properties":[{"key":"Number of interactors","value":"3838"}]},{"database":"STRING","id":"3702.F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"F4HYJ7","properties":[{"key":"glycosylation","value":"1 site"}]},{"database":"iPTMnet","id":"F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"224077","properties":[{"key":"Description","value":"-"}],"isoformId":"F4HYJ7-1"},{"database":"GeneID","id":"841287","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G48650","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G48650","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G48650","properties":[{"key":"GeneName","value":"-"}]},{"database":"eggNOG","id":"KOG0920","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"LENIDEW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PRO","id":"PR:F4HYJ7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"F4HYJ7","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:RHEA"}]},{"database":"GO","id":"GO:0004386","properties":[{"key":"GoTerm","value":"F:helicase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0003729","properties":[{"key":"GoTerm","value":"F:mRNA binding"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"32344669"}]},{"database":"GO","id":"GO:0003724","properties":[{"key":"GoTerm","value":"F:RNA helicase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"CDD","id":"cd17917","properties":[{"key":"EntryName","value":"DEXHc_RHA-like"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd00048","properties":[{"key":"EntryName","value":"DSRM_SF"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd18791","properties":[{"key":"EntryName","value":"SF2_C_RHA"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000480","properties":[{"key":"EntryName","value":"DExH-box ATP-dependent RNA helicase DExH3"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000526","properties":[{"key":"EntryName","value":"DExH-box ATP-dependent RNA helicase DExH3"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.120.1080:FF:000002","properties":[{"key":"EntryName","value":"Putative ATP-dependent RNA helicase DHX36"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.120.1080","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.160.20","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR011709","properties":[{"key":"EntryName","value":"DEAD-box_helicase_OB_fold"}]},{"database":"InterPro","id":"IPR011545","properties":[{"key":"EntryName","value":"DEAD/DEAH_box_helicase_dom"}]},{"database":"InterPro","id":"IPR059863","properties":[{"key":"EntryName","value":"DEXH1_3_5_N"}]},{"database":"InterPro","id":"IPR014720","properties":[{"key":"EntryName","value":"dsRBD_dom"}]},{"database":"InterPro","id":"IPR048333","properties":[{"key":"EntryName","value":"HA2_WH"}]},{"database":"InterPro","id":"IPR007502","properties":[{"key":"EntryName","value":"Helicase-assoc_dom"}]},{"database":"InterPro","id":"IPR014001","properties":[{"key":"EntryName","value":"Helicase_ATP-bd"}]},{"database":"InterPro","id":"IPR001650","properties":[{"key":"EntryName","value":"Helicase_C-like"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR059023","properties":[{"key":"EntryName","value":"RNA_hel_CTD"}]},{"database":"PANTHER","id":"PTHR18934","properties":[{"key":"EntryName","value":"ATP-DEPENDENT RNA HELICASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR18934:SF229","properties":[{"key":"EntryName","value":"DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00270","properties":[{"key":"EntryName","value":"DEAD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF27261","properties":[{"key":"EntryName","value":"DEXH1_3_5_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00035","properties":[{"key":"EntryName","value":"dsrm"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21010","properties":[{"key":"EntryName","value":"HA2_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00271","properties":[{"key":"EntryName","value":"Helicase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07717","properties":[{"key":"EntryName","value":"OB_NTP_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF26026","properties":[{"key":"EntryName","value":"RNA_hel_CTD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04408","properties":[{"key":"EntryName","value":"WHD_HA2"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00487","properties":[{"key":"EntryName","value":"DEXDc"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00847","properties":[{"key":"EntryName","value":"HA2"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00490","properties":[{"key":"EntryName","value":"HELICc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF54768","properties":[{"key":"EntryName","value":"dsRNA-binding domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51192","properties":[{"key":"EntryName","value":"HELICASE_ATP_BIND_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51194","properties":[{"key":"EntryName","value":"HELICASE_CTER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQLYSAIFRGHIRPNAVVGAASMFIQHNSIQLHRSPKLLLRPSSVVRSLHCRRSGGLVTHSQRSRVLCVKAARGDASSSTLGIEWRAANLPYFQRQNSGYGRIAYNDYESSDESDRDVGSSQSQQMAGSTLDNIDQWRFKLTMLLRNKEDQEVVSRERKDRRDFDHISALATRMGLHSRQYSKIVVISKAPLPNYRPDLDDKRPQREVVLPFGLQSEVDAHLHSFLDQKKTLIPEMPRQNSSESLANGYGNYETPETVMQNSLARERILRPRSLQLKSKQQQWVDSPEGQKMVGFRKTLPAYKEKDALLKAIAANQVVVVSGETGCGKTTQLPQYILESEIEAARGATCSIICTQPRRISAISVSERVAAERGEQIGESVGYKVRLEGMRGRDTRLLFCTTGVLLRRLLVDRSLKGVTHVVVDEIHERGMNEDFLLIVLKDLLPRRPDLKLILMSATLNAELFSSYFGGAPAMHIPGFTYPVRAHFLEDYLETSGYRLTTYNQIDDYGEEKTWKMQKQAQFKKRKSLISSAVEDALEAADFKGYNFRTRDSLSCWSPDSIGFNLIENVLCHIVKGERPGAVLVFMTGWDDINSLKNQLEAHSLLGDPNKVLLLACHGSMASSEQRLIFDRPPEGIRKIVLATNMAETSITINDVVYVIDCGKAKETSYDALNNTPCLLPSWISKAAARQRRGRAGRVMPGECYHLYPRCVYEAFADYQQPELLRTPLQSLCLQIKSLGLGSISEFLSRALQPPEALSVQNAVEYLKIIGALDDDENLTPLGKNLSMLPVEPKLGKMLILGAIFNCLDPVMTVVAGLSVRDPFLMPFDKKDLAETARSKFSGRDYSDHLTLVRAYNGWKDAERTHSGYDYCWKNFLSSQTLKAMDSMRKQFFNLLKEASLIDNIEGCSKLSHDEHLVRAIICAGMFPGVCSVVNKEKSITLKTMEDGQVLLYSSSVNGNVPMIPFPWLVFNDKVKVNSVFLRDSTAVSDSVLLLFGDKISSGGFDGHLKMLGGYLEFFMKPTLAYTYLSLKRELDELIQNKLVNPKLDIQLYDKLMTAIRLLVSEDQCEGRFVYGRKALSPTPAKKLKDVGAQLQNSGGENNKNQLQTLLARAGHGSPVYKTRQLKNNQFRSMVTFNGLDFMGKPCGSKKNAEKDAAHEALLWLQGESKSSLNDLNHMSMLLKKNKSKNHAKASTKWG","length":1197,"molWeight":134253,"crc64":"4C93472E210E6283","md5":"FCF629C47E912E10F478233C98C382B2"},"extraAttributes":{"countByCommentType":{"CATALYTIC ACTIVITY":1,"ALTERNATIVE PRODUCTS":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":2,"Motif":1,"Binding site":1},"uniParcId":"UPI0000197023"}}