{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"D8KIE9","secondaryAccessions":["A2RKH8","Q1RN04"],"uniProtkbId":"RIBU_LACLN","entryAudit":{"firstPublicDate":"2010-11-30","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2010-10-05","entryVersion":58,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Lactococcus lactis subsp. cremoris (strain NZ9000)","taxonId":746361,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Streptococcaceae","Lactococcus","Lactococcus cremoris subsp. cremoris"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Riboflavin transporter RibU"}},"alternativeNames":[{"fullName":{"value":"Riboflavin ECF transporter S component RibU"}}]},"genes":[{"geneName":{"value":"ribU"},"orderedLocusNames":[{"value":"LLNZ_06150"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16585736"}],"value":"Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins (By similarity). Mediates riboflavin uptake, probably also transports FMN and roseoflavin"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16585736"}],"value":"Inhibited by FMN and roseoflavin"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Forms a stable energy-coupling factor (ECF) transporter complex composed of a membrane-embedded substrate-binding protein (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component). May be able to interact with more than 1 S component at a time (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17289680"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17289680"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16585736"}],"value":"Induced by riboflavin deficiency"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17289680"}],"value":"Riboflavin is stacked with one or more Trp residues in the binding pocket of RibU"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16585736"}],"value":"Mutant exhibits altered transcriptional control of the riboflavin biosynthesis operon in response to riboflavin and FMN, and does not consume riboflavin from the growth medium"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"value":"Riboflavin, FMN and roseoflavin, but not FAD, bind to RibU with high affinity and 1:1 stoichiometry"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"Riboflavin transporter RibU","featureId":"PRO_0000402187"},{"type":"Transmembrane","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":169,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"Strong increase of the riboflavin dissociation constant.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17289680"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"No change in riboflavin binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17289680"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"No change in riboflavin binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17289680"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["Y"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"16585736","citationType":"journal article","authors":["Burgess C.M.","Slotboom D.J.","Geertsma E.R.","Duurkens R.H.","Poolman B.","van Sinderen D."],"citationCrossReferences":[{"database":"PubMed","id":"16585736"},{"database":"DOI","id":"10.1128/jb.188.8.2752-2760.2006"}],"title":"The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.","publicationDate":"2006","journal":"J. 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