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It has slow DAC activity with ADP as a substrate and may have weak ADPase activity (PubMed:26441857). Required for efficient spore formation, whereas in B.subtilis, it is required for efficient spore germination. It is produced under the control of different sigma factors in the two bacteria. It is also required for parasporal crystal formation (PubMed:26441857)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 ATP = 3',3'-c-di-AMP + 2 diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:35655"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:71500"}],"ecNumber":"2.7.7.85","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00838"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26441857"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26441857"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26441857"}],"value":"Optimum pH is 7.5."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"26441857"}],"value":"Probably forms a homohexamer (PubMed:26441857)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"26441857"}],"value":"Probably expressed only during sporulation"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26441857"}],"value":"First expressed at onset of sporulation, maximal transcription at 18 hours during sporulation in mid-stationary phase. Under control of sigma-H factor; in B.subtilis is under control of the sigma-G factor"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26441857"}],"value":"The N-terminal region (residues 1-69) is required for activity, unlike the B.subtilis ortholog where their removal increases c-di-AMP production"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26441857"}],"value":"Spore formation delayed by about 2 hours, about 20-fold decrease in spore formation; 2-fold decrease in parasporal crystal formation, 30% decreased c-di-AMP levels at 18 hours growth. 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