{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"D4GQN6","uniProtkbId":"CAS7_HALVD","entryAudit":{"firstPublicDate":"2015-03-04","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2010-05-18","entryVersion":61,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)","commonName":"Halobacterium volcanii","taxonId":309800,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Stenosarchaea group","Halobacteria","Halobacteriales","Haloferacaceae","Haloferax"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24459147"}],"value":"CRISPR-associated protein Cas7"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24459147"}],"value":"cas7"},"synonyms":[{"value":"csh2"}],"orderedLocusNames":[{"value":"HVO_A0207"}],"orfNames":[{"value":"C498_09711"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22767603"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Plasmid targeted by CRISPR locus P1 transform wild-type cells very poorly (PubMed:22767603). This protein helps process or stabilize pre-crRNA into individual crRNA units, in vivo Cas6 and Cas7 are also required for optimal crRNA processing and/or stability (PubMed:24459147)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"Component of the Cascade-like complex (Cascade I-B), composed of Cas5, Cas6, Cas7 and crRNA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22767603"}],"value":"Loss of the 8 Cas genes in this locus (cas1, cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR interference against plasmid targeted by this CRISPR locus, i.e. plasmid is not destroyed by CRISPR"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20333302"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24459147"}],"value":"There are 3 CRISPR RNA loci in this organism and a single cas gene locus. 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Biol. Chem.","firstPage":"33351","lastPage":"33363","volume":"287"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"DS2 / DS70 / H26","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"24459147","citationType":"journal article","authors":["Brendel J.","Stoll B.","Lange S.J.","Sharma K.","Lenz C.","Stachler A.E.","Maier L.K.","Richter H.","Nickel L.","Schmitz R.A.","Randau L.","Allers T.","Urlaub H.","Backofen R.","Marchfelder A."],"citationCrossReferences":[{"database":"PubMed","id":"24459147"},{"database":"DOI","id":"10.1074/jbc.m113.508184"}],"title":"A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii.","publicationDate":"2014","journal":"J. Biol. 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