{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"D4GQN4","uniProtkbId":"CAS6_HALVD","entryAudit":{"firstPublicDate":"2015-03-04","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2010-05-18","entryVersion":53,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)","commonName":"Halobacterium volcanii","taxonId":309800,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Stenosarchaea group","Halobacteria","Halobacteriales","Haloferacaceae","Haloferax"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24459147"}],"value":"CRISPR-associated endoribonuclease Cas6"},"ecNumbers":[{"value":"3.1.-.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24459147"}],"value":"cas6"},"orderedLocusNames":[{"value":"HVO_A0205"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22767603"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Plasmid targeted by CRISPR locus P1 transform wild-type cells very poorly (PubMed:22767603). This protein processes pre-crRNA into individual crRNA units, in vivo at least Cas5 and Cas7 are also required for optimal crRNA processing and/or stability (PubMed:24459147)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"Component of the Cascade-like complex (Cascade I-B), composed of Cas5, Cas6, Cas7 and crRNA"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"Expressed in exponential and stationary phases"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22767603"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"value":"Loss of the 8 Cas genes in this locus (cas1, cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR interference against plasmid targeted by this CRISPR locus, i.e. plasmid is not destroyed by CRISPR (PubMed:22767603). Upon disruption of only this gene, no visible phenotype when grown under differing temperature and salt regimes. Does not produce mature length crRNA. Can be complemented by endogenous Cas6, but not by Cas6 from halophilic archaea H.lacusprofundi, or non-halophilic archaea M.maripaludis or M.mazei. Loss of CRISPR interference against plasmid targeted by this CRISPR locus, i.e. plasmid is not destroyed by CRISPR action (PubMed:24459147)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20333302"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24459147"}],"value":"There are 3 CRISPR RNA loci in this organism and a single cas gene locus. A CRISPR-Cas type I-B system"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"CRISPR-associated endoribonuclease Cas6","featureId":"PRO_0000432153"},{"type":"Active site","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24459147"}]},{"type":"Mutagenesis","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"About 40% of mature crRNA produced, 20% reduced CRISPR plasmid interference.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"About 130% of mature crRNA produced, CRISPR plasmid interference restored in a deletion mutant.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"About 160% of mature crRNA produced, CRISPR plasmid interference restored in a deletion mutant.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":256,"modifier":"EXACT"}},"description":"About 10% of mature crRNA produced, no CRISPR plasmid interefence observed.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"No mature crRNA produced, no CRISPR plasmid interefence observed.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}}],"geneLocations":[{"value":"pHV4","geneEncodingType":"Plasmid"}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0614","category":"Technical term","name":"Plasmid"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"}],"references":[{"referenceNumber":1,"citation":{"id":"20333302","citationType":"journal article","authors":["Hartman A.L.","Norais C.","Badger J.H.","Delmas S.","Haldenby S.","Madupu R.","Robinson J.","Khouri H.","Ren Q.","Lowe T.M.","Maupin-Furlow J.","Pohlschroder M.","Daniels C.","Pfeiffer F.","Allers T.","Eisen J.A."],"citationCrossReferences":[{"database":"PubMed","id":"20333302"},{"database":"DOI","id":"10.1371/journal.pone.0009605"}],"title":"The complete genome sequence of Haloferax volcanii DS2, a model archaeon.","publicationDate":"2010","journal":"PLoS ONE","firstPage":"E9605","lastPage":"E9605","volume":"5"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"22767603","citationType":"journal article","authors":["Fischer S.","Maier L.K.","Stoll B.","Brendel J.","Fischer E.","Pfeiffer F.","Dyall-Smith M.","Marchfelder A."],"citationCrossReferences":[{"database":"PubMed","id":"22767603"},{"database":"DOI","id":"10.1074/jbc.m112.377002"}],"title":"An archaeal immune system can detect multiple protospacer adjacent motifs (PAMs) to target invader DNA.","publicationDate":"2012","journal":"J. Biol. Chem.","firstPage":"33351","lastPage":"33363","volume":"287"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"DS2 / DS70 / H26","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24459147","citationType":"journal article","authors":["Brendel J.","Stoll B.","Lange S.J.","Sharma K.","Lenz C.","Stachler A.E.","Maier L.K.","Richter H.","Nickel L.","Schmitz R.A.","Randau L.","Allers T.","Urlaub H.","Backofen R.","Marchfelder A."],"citationCrossReferences":[{"database":"PubMed","id":"24459147"},{"database":"DOI","id":"10.1074/jbc.m113.508184"}],"title":"A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and stability of clustered regularly interspaced short palindromic repeats (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii.","publicationDate":"2014","journal":"J. Biol. Chem.","firstPage":"7164","lastPage":"7177","volume":"289"},"referencePositions":["FUNCTION","SUBUNIT","SUBCELLULAR LOCATION","INDUCTION","DISRUPTION PHENOTYPE","ACTIVE SITE","MUTAGENESIS OF HIS-41; SER-115; SER-224; GLY-256 AND GLY-258"],"referenceComments":[{"value":"DS2 / DS70 / H119","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP001955","properties":[{"key":"ProteinId","value":"ADE01675.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_013035014.1","properties":[{"key":"NucleotideSequenceId","value":"NC_013966.1"}]},{"database":"AlphaFoldDB","id":"D4GQN4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"D4GQN4","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"ADE01675","properties":[{"key":"ProteinId","value":"ADE01675"},{"key":"GeneId","value":"HVO_A0205"}]},{"database":"GeneID","id":"8923584","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hvo:HVO_A0205","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"arCOG04342","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_089858_1_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"43942at2157","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000008243","properties":[{"key":"Component","value":"Plasmid pHV4"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24459147"}]},{"database":"GO","id":"GO:0016788","properties":[{"key":"GoTerm","value":"F:hydrolase activity, acting on ester bonds"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.30.70.1900","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR049435","properties":[{"key":"EntryName","value":"Cas_Cas6_C"}]},{"database":"InterPro","id":"IPR010156","properties":[{"key":"EntryName","value":"CRISPR-assoc_prot_Cas6"}]},{"database":"PANTHER","id":"PTHR36984","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR36984:SF1","properties":[{"key":"EntryName","value":"CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01881","properties":[{"key":"EntryName","value":"Cas_Cas6_C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRIELALDAVADAAYDRSYFHKARGRIWRGLENDDTFSETHDSNHGVGFSYSNIFPWGQIEEGDRRYFRIASPRRGLLDTLIAHFGRERAFEIGQMRFTIDDITGHAPDVGEAGSTGVLETGTGVHCALGRQLAEEYNLDTSKIDAGESETELFWRPKHGMEPLQDAIRRSLQQTHELYGDDYYDGPGEVDEPLFDRIEPIKDDVVYPVRFQPATAVDRTVLLSKWRFGYRVRDETHRYHLNLALDSGIGQRREHGFGFLNLTDKTLPRASD","length":272,"molWeight":31051,"crc64":"357C604A701DC4B0","md5":"5FB9272EEE07550F5728A193E4EEC35F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Mutagenesis":5},"uniParcId":"UPI0001CE4712"}}