{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"D0KWS7","uniProtkbId":"DABA2_HALNC","entryAudit":{"firstPublicDate":"2021-09-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-12-15","entryVersion":55,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Halothiobacillus neapolitanus (strain ATCC 23641 / DSM 15147 / CIP 104769 / NCIMB 8539 / c2)","commonName":"Thiobacillus neapolitanus","taxonId":555778,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Chromatiales","Halothiobacillaceae","Halothiobacillus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01871"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31406332"}],"value":"Probable inorganic carbon transporter subunit DabA2"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01871"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31406332"}],"value":"dabA2"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ACX95074.1"}],"value":"Hneap_0211"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31406332"},{"evidenceCode":"ECO:0000305","source":"Reference","id":"Ref.2"}],"value":"Part of an energy-coupled inorganic carbon pump; its substrate may be carbon dioxide. Expression of both dabA2 and dabB2 (DAB2) restores growth in ambient air to E.coli deleted of its carbonic anhydrase genes (called CAfree, deletion of 'can' and 'cynT'); neither dabA2 or dabB2 alone is sufficient. Rescue is pH-independent, suggesting it transports CO(2) and not carbonate ions. Together the genes allow greater than normal uptake of inorganic carbon by E.coli (PubMed:31406332). Uptake of carbon dioxide rather than bicarbonate has been suggested based on kinetic calculations (Probable)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01871"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31406332"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31406332"},{"evidenceCode":"ECO:0000269","source":"Reference","id":"Ref.2"}],"value":"Uptake of inorganic carbon by cells in the presence of thiosulphate is fully inhibited by the uncouplers carbonyl cyanide m-chlorophenyl hydrazone (CCCP), carbonyl cyanide p-trifluoromethoxyphenyl hydrazone (FCCP), S13 or SF6847. Not inhibited by the ATPase inhibitor N,N-dicyclohexylcarbodiimide (DCCD) (Ref.2). Inorganic carbon uptake is inhibited by the ionophore CCCP, suggesting uptake is coupled to a cation gradient (PubMed:31406332)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31406332"}],"value":"Forms a complex with DabB2, possibly a heterodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01871"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31406332"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01871"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31406332"}],"value":"Peripheral membrane protein","id":"SL-9903"},"orientation":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"31406332"}],"value":"Cytoplasmic 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