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Binds to the surface of ice crystals and inhibits their growth. Has low thermal hysteresis (TH) activity, which is the ability to lower the freezing point of an aqueous solution below its melting point (PubMed:20067781, PubMed:22303017, PubMed:22426061, PubMed:22622645, PubMed:23203635, PubMed:24699650). The TH activity of this protein is approximately 0.2 degrees Celsius at 50 uM and 0.3 degrees Celsius at 400 uM (PubMed:24699650)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22303017"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22426061"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24699650"}],"value":"Homodimer (PubMed:22303017, PubMed:22426061, PubMed:24699650). 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Glycosylation may increase stability and secretion of this protein (Probable)"}],"commentType":"PTM"},{"commentType":"MASS SPECTROMETRY","method":"MALDI","molWeight":25565.0,"molWeightError":0.0,"note":"The measured mass is that of the mature protein.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23203635"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22622645"}],"value":"Improves cryopreservation of the human red blood cells (RBCs). Almost 90 % hemolysis damage induced to RBCs by freeze thawing with the slow warming at 22 degrees Celsius is dramatically decreased to less than 16 % when 0.4 or 0.8 mg/ml of this protein is used as a cryoprotectant. The post-thaw cell counts of RBCs frozen in the presence of 0.8 mg/ml of this protein and thawed by slow warming at 22 degrees Celsius is almost the same as that of non-frozen intact RBCs, whereas the recovery of RBCs frozen in its absence is less than 30 %"}],"commentType":"BIOTECHNOLOGY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ice-binding protein family"}],"commentType":"SIMILARITY"},{"commentType":"WEB RESOURCE","resourceName":"Protein Spotlight","resourceUrl":"https://www.proteinspotlight.org/back_issues/209/","ftp":false,"note":"Ice whisperer - Issue 209 of December 2018"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22426061"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23203635"}]},{"type":"Chain","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"Ice-binding protein","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_5011088933"},{"type":"Site","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Ice-binding","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22303017"}]},{"type":"Site","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"Ice-binding","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22303017"}]},{"type":"Site","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":234,"modifier":"EXACT"}},"description":"Ice-binding","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22303017"}]},{"type":"Glycosylation","location":{"start":{"value":185,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22303017"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"3UYV"}],"featureId":""},{"type":"Mutagenesis","location":{"start":{"value":43,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Has 35% thermal hysteresis (TH) activity of that of the wild-type.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22303017"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"Has 89% thermal hysteresis (TH) activity of that of the wild-type.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22303017"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"Has 28% thermal hysteresis (TH) activity of that of the wild-type.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22303017"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":109,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"Has 92% thermal hysteresis (TH) activity of that of the wild-type.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22303017"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["Y"]}},{"type":"Mutagenesis","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"Has 62% thermal hysteresis (TH) activity of that of the wild-type. 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