{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C6XZB6","secondaryAccessions":["Q46080"],"uniProtkbId":"HEPB_PEDHD","entryAudit":{"firstPublicDate":"2013-10-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-09-22","entryVersion":66,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)","taxonId":485917,"lineage":["Bacteria","Pseudomonadati","Bacteroidota","Sphingobacteriia","Sphingobacteriales","Sphingobacteriaceae","Pedobacter"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Heparin and heparin-sulfate lyase"}},"alternativeNames":[{"fullName":{"value":"Heparin lyase"},"ecNumbers":[{"value":"4.2.2.7"}]},{"fullName":{"value":"Heparin-sulfate lyase"},"ecNumbers":[{"value":"4.2.2.8"}]},{"fullName":{"value":"Heparinase II"},"shortNames":[{"value":"HepII"}]}],"flag":"Precursor"},"genes":[{"geneName":{"value":"hepB"},"orderedLocusNames":[{"value":"Phep_2408"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8702264"}],"value":"Cleaves both heparin and heparan sulfate glycosaminoglycans through a beta-elimination mechanism. Cleaves heparin at alpha-D-GlcNp2S6S(1->4) alpha-L-IdoAp2S and heparan sulfate at alpha-D-GlcNp2Ac(or 2S)6OH(1->4)beta-D-GlcAp"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Elimination of sulfate, appears to act on linkages between N-acetyl-D-glucosamine and uronate. Product is an unsaturated sugar.","ecNumber":"4.2.2.8","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8702264"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Eliminative cleavage of polysaccharides containing (1->4)-linked D-glucuronate or L-iduronate residues and (1->4)-alpha-linked 2-sulfoamino-2-deoxy-6-sulfo-D-glucose residues to give oligosaccharides with terminal 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends.","ecNumber":"4.2.2.7","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8702264"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16565082"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8702264"}],"value":"Periplasm","id":"SL-0200"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the polysaccharide lyase 12 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8702264"}]},{"type":"Chain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":772,"modifier":"EXACT"}},"description":"Heparin and heparin-sulfate lyase","featureId":"PRO_5000144613"},{"type":"Active site","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20404324"}]},{"type":"Active site","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20404324"}]},{"type":"Active site","location":{"start":{"value":406,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20404324"}]},{"type":"Binding site","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":205,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":307,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":405,"modifier":"EXACT"},"end":{"value":405,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":408,"modifier":"EXACT"},"end":{"value":408,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16565082"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":425,"modifier":"EXACT"},"end":{"value":425,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16565082"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":429,"modifier":"EXACT"},"end":{"value":429,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":436,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Binding site","location":{"start":{"value":451,"modifier":"EXACT"},"end":{"value":451,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16565082"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":470,"modifier":"EXACT"},"end":{"value":470,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:506227"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"ligand":{"name":"N-acetyl-D-glucosamine","id":"ChEBI:CHEBI:506227"}},{"type":"Glycosylation","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"O-linked (Man...) threonine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16565082"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"featureId":""},{"type":"Mutagenesis","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":257,"modifier":"EXACT"},"end":{"value":257,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":406,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":429,"modifier":"EXACT"},"end":{"value":429,"modifier":"EXACT"}},"description":"Impaired catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20404324"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["A","F"]}},{"type":"Sequence conflict","location":{"start":{"value":505,"modifier":"EXACT"},"end":{"value":505,"modifier":"EXACT"}},"description":"in Ref. 1; AA sequence","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":758,"modifier":"EXACT"},"end":{"value":758,"modifier":"EXACT"}},"description":"in Ref. 1; AAB18277","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["P"]}},{"type":"Helix","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E80"}]},{"type":"Beta strand","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":37,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":43,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FUT"}]},{"type":"Helix","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":118,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":137,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":144,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":170,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":191,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":198,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":202,"modifier":"EXACT"},"end":{"value":204,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":207,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":219,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":225,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":246,"modifier":"EXACT"},"end":{"value":248,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":254,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":277,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":287,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":290,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":312,"modifier":"EXACT"},"end":{"value":314,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":319,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":332,"modifier":"EXACT"},"end":{"value":339,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":346,"modifier":"EXACT"},"end":{"value":348,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":355,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":365,"modifier":"EXACT"},"end":{"value":367,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":370,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":375,"modifier":"EXACT"},"end":{"value":378,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":380,"modifier":"EXACT"},"end":{"value":385,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":392,"modifier":"EXACT"},"end":{"value":397,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":414,"modifier":"EXACT"},"end":{"value":423,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":429,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":441,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":448,"modifier":"EXACT"},"end":{"value":450,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":451,"modifier":"EXACT"},"end":{"value":457,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":468,"modifier":"EXACT"},"end":{"value":472,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":487,"modifier":"EXACT"},"end":{"value":489,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":500,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":501,"modifier":"EXACT"},"end":{"value":503,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":508,"modifier":"EXACT"},"end":{"value":516,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":518,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":522,"modifier":"EXACT"},"end":{"value":529,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":531,"modifier":"EXACT"},"end":{"value":533,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":538,"modifier":"EXACT"},"end":{"value":549,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":553,"modifier":"EXACT"},"end":{"value":555,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FUQ"}]},{"type":"Beta strand","location":{"start":{"value":557,"modifier":"EXACT"},"end":{"value":569,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":572,"modifier":"EXACT"},"end":{"value":582,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":585,"modifier":"EXACT"},"end":{"value":587,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":590,"modifier":"EXACT"},"end":{"value":594,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":602,"modifier":"EXACT"},"end":{"value":611,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":613,"modifier":"EXACT"},"end":{"value":615,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":616,"modifier":"EXACT"},"end":{"value":623,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":624,"modifier":"EXACT"},"end":{"value":628,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":638,"modifier":"EXACT"},"end":{"value":640,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":651,"modifier":"EXACT"},"end":{"value":660,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":663,"modifier":"EXACT"},"end":{"value":675,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Helix","location":{"start":{"value":682,"modifier":"EXACT"},"end":{"value":684,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":685,"modifier":"EXACT"},"end":{"value":688,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":690,"modifier":"EXACT"},"end":{"value":697,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":700,"modifier":"EXACT"},"end":{"value":705,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":715,"modifier":"EXACT"},"end":{"value":719,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":721,"modifier":"EXACT"},"end":{"value":723,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2FUQ"}]},{"type":"Beta strand","location":{"start":{"value":725,"modifier":"EXACT"},"end":{"value":729,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":734,"modifier":"EXACT"},"end":{"value":741,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":744,"modifier":"EXACT"},"end":{"value":752,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Turn","location":{"start":{"value":754,"modifier":"EXACT"},"end":{"value":756,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":759,"modifier":"EXACT"},"end":{"value":763,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]},{"type":"Beta strand","location":{"start":{"value":765,"modifier":"EXACT"},"end":{"value":771,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3E7J"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"8702264","citationType":"journal article","authors":["Su H.","Blain F.","Musil R.A.","Zimmermann J.J.","Gu K.","Bennett D.C."],"citationCrossReferences":[{"database":"PubMed","id":"8702264"},{"database":"DOI","id":"10.1128/aem.62.8.2723-2734.1996"}],"title":"Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum.","publicationDate":"1996","journal":"Appl. Environ. Microbiol.","firstPage":"2723","lastPage":"2734","volume":"62"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PROTEIN SEQUENCE OF N-TERMINUS","PROTEIN SEQUENCE OF 85-96; 222-233 AND 500-526","FUNCTION","CATALYTIC ACTIVITY","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"21304637","citationType":"journal article","authors":["Han C.","Spring S.","Lapidus A.","Del Rio T.G.","Tice H.","Copeland A.","Cheng J.F.","Lucas S.","Chen F.","Nolan M.","Bruce D.","Goodwin L.","Pitluck S.","Ivanova N.","Mavromatis K.","Mikhailova N.","Pati A.","Chen A.","Palaniappan K.","Land M.","Hauser L.","Chang Y.J.","Jeffries C.C.","Saunders E.","Chertkov O.","Brettin T.","Goker M.","Rohde M.","Bristow J.","Eisen J.A.","Markowitz V.","Hugenholtz P.","Kyrpides N.C.","Klenk H.P.","Detter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"21304637"},{"database":"DOI","id":"10.4056/sigs.22138"}],"title":"Complete genome sequence of Pedobacter heparinus type strain (HIM 762-3).","publicationDate":"2009","journal":"Stand. Genomic Sci.","firstPage":"54","lastPage":"62","volume":"1"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"2211596","citationType":"journal article","authors":["Nader H.B.","Porcionatto M.A.","Tersariol I.L.","Pinhal M.A.","Oliveira F.W.","Moraes C.T.","Dietrich C.P."],"citationCrossReferences":[{"database":"PubMed","id":"2211596"},{"database":"DOI","id":"10.1016/s0021-9258(17)44833-2"}],"title":"Purification and substrate specificity of heparitinase I and heparitinase II from Flavobacterium heparinum. Analyses of the heparin and heparan sulfate degradation products by 13C NMR spectroscopy.","publicationDate":"1990","journal":"J. Biol. Chem.","firstPage":"16807","lastPage":"16813","volume":"265"},"referencePositions":["IDENTIFICATION"]},{"referenceNumber":4,"citation":{"id":"16565082","citationType":"journal article","authors":["Shaya D.","Tocilj A.","Li Y.","Myette J.","Venkataraman G.","Sasisekharan R.","Cygler M."],"citationCrossReferences":[{"database":"PubMed","id":"16565082"},{"database":"DOI","id":"10.1074/jbc.m512055200"}],"title":"Crystal structure of heparinase II from Pedobacter heparinus and its complex with a disaccharide product.","publicationDate":"2006","journal":"J. Biol. Chem.","firstPage":"15525","lastPage":"15535","volume":"281"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 27-772 IN COMPLEX WITH PHOSPHATE AND ZINC","GLYCOSYLATION AT THR-134","SUBUNIT"]},{"referenceNumber":5,"citation":{"id":"20404324","citationType":"journal article","authors":["Shaya D.","Zhao W.","Garron M.L.","Xiao Z.","Cui Q.","Zhang Z.","Sulea T.","Linhardt R.J.","Cygler M."],"citationCrossReferences":[{"database":"PubMed","id":"20404324"},{"database":"DOI","id":"10.1074/jbc.m110.101071"}],"title":"Catalytic mechanism of heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate.","publicationDate":"2010","journal":"J. Biol. Chem.","firstPage":"20051","lastPage":"20061","volume":"285"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 24-772 IN COMPLEX WITH N-ACETYL-D-GLUCOSAMINE AND ZINC","GLYCOSYLATION AT THR-134","ACTIVE SITE","MUTAGENESIS OF HIS-202; TYR-257; HIS-406 AND TYR-429"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"U27585","properties":[{"key":"ProteinId","value":"AAB18277.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP001681","properties":[{"key":"ProteinId","value":"ACU04612.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_015808224.1","properties":[{"key":"NucleotideSequenceId","value":"NC_013061.1"}]},{"database":"PDB","id":"2FUQ","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.15 A"},{"key":"Chains","value":"A/B=26-772"}]},{"database":"PDB","id":"2FUT","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"A/B=25-772"}]},{"database":"PDB","id":"3E7J","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"A/B=24-772"}]},{"database":"PDB","id":"3E80","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.35 A"},{"key":"Chains","value":"A/B/C=24-772"}]},{"database":"PDBsum","id":"2FUQ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2FUT","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3E7J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3E80","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"C6XZB6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"C6XZB6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"485917.Phep_2408","properties":[{"key":"Description","value":"-"}]},{"database":"CAZy","id":"PL21","properties":[{"key":"FamilyName","value":"Polysaccharide Lyase Family 21"}]},{"database":"GlyCosmos","id":"C6XZB6","properties":[{"key":"glycosylation","value":"1 site, No reported glycans"}]},{"database":"iPTMnet","id":"C6XZB6","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"phe:Phep_2408","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG5652","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_389651_0_0_10","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9147455at2","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-19211","properties":[{"key":"Description","value":"-"}]},{"database":"EvolutionaryTrace","id":"C6XZB6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000852","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0042597","properties":[{"key":"GoTerm","value":"C:periplasmic space"},{"key":"GoEvidenceType","value":"TAS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000304","source":"PubMed","id":"8702264"}]},{"database":"GO","id":"GO:0008201","properties":[{"key":"GoTerm","value":"F:heparin binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8702264"}]},{"database":"GO","id":"GO:0047488","properties":[{"key":"GoTerm","value":"F:heparin lyase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8702264"}]},{"database":"GO","id":"GO:0015021","properties":[{"key":"GoTerm","value":"F:heparin-sulfate lyase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0030211","properties":[{"key":"GoTerm","value":"P:heparin proteoglycan catabolic process"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"8702264"}]},{"database":"FunFam","id":"2.70.98.70:FF:000002","properties":[{"key":"EntryName","value":"Heparin and heparin-sulfate lyase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.2750","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.70.98.70","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.50.10.100","properties":[{"key":"EntryName","value":"Chondroitin AC/alginate lyase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008929","properties":[{"key":"EntryName","value":"Chondroitin_lyas"}]},{"database":"InterPro","id":"IPR032518","properties":[{"key":"EntryName","value":"DSE-like_N"}]},{"database":"InterPro","id":"IPR012480","properties":[{"key":"EntryName","value":"Hepar_II_III_C"}]},{"database":"InterPro","id":"IPR054645","properties":[{"key":"EntryName","value":"HepB"}]},{"database":"InterPro","id":"IPR040925","properties":[{"key":"EntryName","value":"HepII_C"}]},{"database":"NCBIfam","id":"NF045571","properties":[{"key":"EntryName","value":"HepHepsulflyase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16332","properties":[{"key":"EntryName","value":"DSE-like_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07940","properties":[{"key":"EntryName","value":"Hepar_II_III_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF18675","properties":[{"key":"EntryName","value":"HepII_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF48230","properties":[{"key":"EntryName","value":"Chondroitin AC/alginate lyase"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKRQLYLYVIFVVVELMVFTTKGYSQTKADVVWKDVDGVSMPIPPKTHPRLYLREQQVPDLKNRMNDPKLKKVWADMIKMQEDWKPADIPEVKDFRFYFNQKGLTVRVELMALNYLMTKDPKVGREAITSIIDTLETATFKPAGDISRGIGLFMVTGAIVYDWCYDQLKPEEKTRFVKAFVRLAKMLECGYPPVKDKSIVGHASEWMIMRDLLSVGIAIYDEFPEMYNLAAGRFFKEHLVARNWFYPSHNYHQGMSYLNVRFTNDLFALWILDRMGAGNVFNPGQQFILYDAIYKRRPDGQILAGGDVDYSRKKPKYYTMPALLAGSYYKDEYLNYEFLKDPNVEPHCKLFEFLWRDTQLGSRKPDDLPLSRYSGSPFGWMIARTGWGPESVIAEMKVNEYSFLNHQHQDAGAFQIYYKGPLAIDAGSYTGSSGGYNSPHNKNFFKRTIAHNSLLIYDPKETFSSSGYGGSDHTDFAANDGGQRLPGKGWIAPRDLKEMLAGDFRTGKILAQGFGPDNQTPDYTYLKGDITAAYSAKVKEVKRSFLFLNLKDAKVPAAMIVFDKVVASNPDFKKFWLLHSIEQPEIKGNQITIKRTKNGDSGMLVNTALLPDAANSNITSIGGKGKDFWVFGTNYTNDPKPGTDEALERGEWRVEITPKKAAAEDYYLNVIQIADNTQQKLHEVKRIDGDKVVGVQLADRIVTFSKTSETVDRPFGFSVVGKGTFKFVMTDLLPGTWQVLKDGKILYPALSAKGDDGALYFEGTEGTYRFLR","length":772,"molWeight":87626,"crc64":"66D9752035421B99","md5":"A1342B73B5518B10EE569EE34C91E43E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Active site":3,"Binding site":14,"Glycosylation":1,"Mutagenesis":4,"Sequence conflict":2,"Helix":36,"Beta strand":37,"Turn":8},"uniParcId":"UPI0001B17D22"}}