{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"C4JF73","uniProtkbId":"UNCB_UNCRE","entryAudit":{"firstPublicDate":"2026-01-28","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2009-07-07","entryVersion":60,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Uncinocarpus reesii (strain UAMH 1704)","commonName":"Gymnoascus siglerae","taxonId":336963,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Onygenales","Onygenaceae","Uncinocarpus"]},"proteinExistence":"4: Predicted","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"40387549"}],"value":"Isoindolinone tripeptide biosynthesis cluster protein B"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"40387549"}],"value":"uncB"},"orfNames":[{"value":"UREG_02295"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40387549"}],"value":"Hypothetical protein; part of the unc gene cluster that mediates the biosynthesis of the tripeptide D-Isd-L-Ala-L-Gln called isoindolamide B (PubMed:40387549). UncB is an hypothetical protein for which the function within the pathway is unknown (PubMed:40387549). The alanine racemase uncE first catalyzes the interconversion between L-alanine and D-alanine. D-alanine is then activated by the NRPS uncD that transfers it to the PKS uncA to produce R-dihydroxymethyl isoindolinone. UncF then catalyzes the gamma-replacement of acetate in O-acetyl-L-homoserine (OAH) with R-dihydroxymethyl isoindolinone to generate the amino acid L-O-isoindolinone-homoserine (L-Isd). Then, the ATP-grasp enzyme uncK acts as an amide-bond-forming enzyme that activates the carboxylate of L-Leu for condensation with the amino group in L-Isd to give the dipeptide L-Leu-L-Isd. Epimerase uncI converts L-Leu-L-Isd to L-Leu-D-Isd in which C-alpha of the Isd residue is epimerized from L to D. UncH is able to hydrolyze dipeptides L-Leu-L-Isd or L-Leu-D-Isd to yield L-Isd or D-Isd, respectively, along with L-Leu. UncG then produces the tetrapeptide L-Leu-D-Isd-L-Ala-L-Gln by adding L-Ala-L-Gln produced by the dipeptide synthetase uncJ that forms L-Ala-L-Gln from L-Ala and L-Gln. Finally, hydrolysis of L-Leu-D-Isd-L-Ala-L-Gln by uncH leads to the final tripeptide D-Isd-L-Ala-L-Gln called isoindolamide B (PubMed:40387549)"}],"commentType":"FUNCTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":623,"modifier":"EXACT"}},"description":"Isoindolinone tripeptide biosynthesis cluster protein B","featureId":"PRO_0000462908"}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"19717792","citationType":"journal article","authors":["Sharpton T.J.","Stajich J.E.","Rounsley S.D.","Gardner M.J.","Wortman J.R.","Jordar V.S.","Maiti R.","Kodira C.D.","Neafsey D.E.","Zeng Q.","Hung C.-Y.","McMahan C.","Muszewska A.","Grynberg M.","Mandel M.A.","Kellner E.M.","Barker B.M.","Galgiani J.N.","Orbach M.J.","Kirkland T.N.","Cole G.T.","Henn M.R.","Birren B.W.","Taylor J.W."],"citationCrossReferences":[{"database":"PubMed","id":"19717792"},{"database":"DOI","id":"10.1101/gr.087551.108"}],"title":"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives.","publicationDate":"2009","journal":"Genome Res.","firstPage":"1722","lastPage":"1731","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"UAMH 1704","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"40387549","citationType":"journal article","authors":["Zhang Y.","Wu L.","Wang Z.","Han W.","Kerr T.A.","Tang Y."],"citationCrossReferences":[{"database":"PubMed","id":"40387549"},{"database":"DOI","id":"10.1021/jacs.5c03321"}],"title":"Genome Mining of Isoindolinone-Containing Peptide Natural Products.","publicationDate":"2025","journal":"J. Am. Chem. Soc.","firstPage":"18923","lastPage":"18933","volume":"147"},"referencePositions":["FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CH476615","properties":[{"key":"ProteinId","value":"EEP77446.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"XP_002542779.1","properties":[{"key":"NucleotideSequenceId","value":"XM_002542733.1"}]},{"database":"AlphaFoldDB","id":"C4JF73","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"336963.C4JF73","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"8441574","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ure:UREG_02295","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"FungiDB:UREG_02295","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0742","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_004471_6_3_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"C4JF73","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"ISFDYVW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"4183084at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002058","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003593","properties":[{"key":"EntryName","value":"AAA+_ATPase"}]},{"database":"InterPro","id":"IPR003959","properties":[{"key":"EntryName","value":"ATPase_AAA_core"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR054289","properties":[{"key":"EntryName","value":"UncB-like_DUF7025"}]},{"database":"PANTHER","id":"PTHR46411:SF3","properties":[{"key":"EntryName","value":"AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46411","properties":[{"key":"EntryName","value":"FAMILY ATPASE, PUTATIVE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00004","properties":[{"key":"EntryName","value":"AAA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22942","properties":[{"key":"EntryName","value":"DUF7025"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00382","properties":[{"key":"EntryName","value":"AAA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSDGNSSLAYRRSVASFSSSLSEMSVDPGYVPDFSVGMRAEVQQLYRPDDRSPWNESVPEKAATDVEAGSYAQECALIVRREPHPITKQIALHSITVQSPLIKKVLDRTFKGLEGVNTQLKQLTFQAPFHIFYYRWHRFEKLCEDEQDQEAKNHLDILYPILSEEVMPHVETMKDLTRNKVITFDYLWTIFAPGMEVYTRFDGQDRLMELTDSRYGANMGGEFFTLELRYIDCDGSSFGYVSSSVDIDKFQGVKKLVDLDAFPAHLHPDMENVVDRLHARGAKLEKLNGFCHMSYSGFYTAKSSRQIRKRHGSGKVENSRIIIDPHTFNLYGTPGPGLGSIGSELDSHADAADEEVFAVIQNVIYRATSQAFQAYQRALERLEKRSKEGISGGCNTASDLFTCSRTMFDTSTSQNPEYEASLALQSSRPWLLPGFQDVGLNPSLAEFYVENVYPIRWNENAFQRLVLPPGYKEIIRAFVQEQLSRDDDFDDIIYGKGRECRPGWRLLVVKQSVNASSTGLGFIMLLSGEPGVGKTLTAESVAEEMRQPLYLMSASELGETATEVEESLEQVLELTSKWNAILLLDECDMFLEARSTADLRRNRLVSGEFRIMTKRKPPENAAS","length":623,"molWeight":70352,"crc64":"D970CCD17C1C5923","md5":"1C2CB9C1F141A4439A0BC2A5F351D4EA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0001A4E8D1"}}