{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"B3LFA3","secondaryAccessions":["Q8LA45","Q9SVN3"],"uniProtkbId":"NIPA2_ARATH","entryAudit":{"firstPublicDate":"2014-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-09-02","entryVersion":109,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable magnesium transporter NIPA2"}}},"genes":[{"orderedLocusNames":[{"value":"At4g13800"}],"orfNames":[{"value":"F18A5.190"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Recruited to the cell membrane in response to low extracellular magnesium"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Early endosome","id":"SL-0094"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NIPA (TC 2.A.7) family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"CAB36844.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"CAB78422.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"Probable magnesium transporter NIPA2","featureId":"PRO_0000430290"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":8,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"Helical; Name=1","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"Helical; Name=2","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"Helical; Name=3","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":101,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Helical; Name=4","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":145,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Helical; Name=5","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"Helical; Name=6","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"Helical; Name=7","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"Helical; Name=8","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":266,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":293,"modifier":"EXACT"}},"description":"Helical; Name=9","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":303,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":330,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":329,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"in Ref. 5; AAM65585","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["H"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"10617198","citationType":"journal article","authors":["Mayer K.F.X.","Schueller C.","Wambutt R.","Murphy G.","Volckaert G.","Pohl T.","Duesterhoeft A.","Stiekema W.","Entian K.-D.","Terryn N.","Harris B.","Ansorge W.","Brandt P.","Grivell L.A.","Rieger M.","Weichselgartner M.","de Simone V.","Obermaier B.","Mache R.","Mueller M.","Kreis M.","Delseny M.","Puigdomenech P.","Watson M.","Schmidtheini T.","Reichert B.","Portetelle D.","Perez-Alonso M.","Boutry M.","Bancroft I.","Vos P.","Hoheisel J.","Zimmermann W.","Wedler H.","Ridley P.","Langham S.-A.","McCullagh B.","Bilham L.","Robben J.","van der Schueren J.","Grymonprez B.","Chuang Y.-J.","Vandenbussche F.","Braeken M.","Weltjens I.","Voet M.","Bastiaens I.","Aert R.","Defoor E.","Weitzenegger T.","Bothe G.","Ramsperger U.","Hilbert H.","Braun M.","Holzer E.","Brandt A.","Peters S.","van Staveren M.","Dirkse W.","Mooijman P.","Klein Lankhorst R.","Rose M.","Hauf J.","Koetter P.","Berneiser S.","Hempel S.","Feldpausch M.","Lamberth S.","Van den Daele H.","De Keyser A.","Buysshaert C.","Gielen J.","Villarroel R.","De Clercq R.","van Montagu M.","Rogers J.","Cronin A.","Quail M.A.","Bray-Allen S.","Clark L.","Doggett J.","Hall S.","Kay M.","Lennard N.","McLay K.","Mayes R.","Pettett A.","Rajandream M.A.","Lyne M.","Benes V.","Rechmann S.","Borkova D.","Bloecker H.","Scharfe M.","Grimm M.","Loehnert T.-H.","Dose S.","de Haan M.","Maarse A.C.","Schaefer M.","Mueller-Auer S.","Gabel C.","Fuchs M.","Fartmann B.","Granderath K.","Dauner D.","Herzl A.","Neumann S.","Argiriou A.","Vitale D.","Liguori R.","Piravandi E.","Massenet O.","Quigley F.","Clabauld G.","Muendlein A.","Felber R.","Schnabl S.","Hiller R.","Schmidt W.","Lecharny A.","Aubourg S.","Chefdor F.","Cooke R.","Berger C.","Monfort A.","Casacuberta E.","Gibbons T.","Weber N.","Vandenbol M.","Bargues M.","Terol J.","Torres A.","Perez-Perez A.","Purnelle B.","Bent E.","Johnson S.","Tacon D.","Jesse T.","Heijnen L.","Schwarz S.","Scholler P.","Heber S.","Francs P.","Bielke C.","Frishman D.","Haase D.","Lemcke K.","Mewes H.-W.","Stocker S.","Zaccaria P.","Bevan M.","Wilson R.K.","de la Bastide M.","Habermann K.","Parnell L.","Dedhia N.","Gnoj L.","Schutz K.","Huang E.","Spiegel L.","Sekhon M.","Murray J.","Sheet P.","Cordes M.","Abu-Threideh J.","Stoneking T.","Kalicki J.","Graves T.","Harmon G.","Edwards J.","Latreille P.","Courtney L.","Cloud J.","Abbott A.","Scott K.","Johnson D.","Minx P.","Bentley D.","Fulton B.","Miller N.","Greco T.","Kemp K.","Kramer J.","Fulton L.","Mardis E.","Dante M.","Pepin K.","Hillier L.W.","Nelson J.","Spieth J.","Ryan E.","Andrews S.","Geisel C.","Layman D.","Du H.","Ali J.","Berghoff A.","Jones K.","Drone K.","Cotton M.","Joshu C.","Antonoiu B.","Zidanic M.","Strong C.","Sun H.","Lamar B.","Yordan C.","Ma P.","Zhong J.","Preston R.","Vil D.","Shekher M.","Matero A.","Shah R.","Swaby I.K.","O'Shaughnessy A.","Rodriguez M.","Hoffman J.","Till S.","Granat S.","Shohdy N.","Hasegawa A.","Hameed A.","Lodhi M.","Johnson A.","Chen E.","Marra M.A.","Martienssen R.","McCombie W.R."],"citationCrossReferences":[{"database":"PubMed","id":"10617198"},{"database":"DOI","id":"10.1038/47134"}],"title":"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.","publicationDate":"1999","journal":"Nature","firstPage":"769","lastPage":"777","volume":"402"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-24PT0JFON6DVH","citationType":"submission","authors":["de los Reyes C.","Quan R.","Chen H.","Bautista V.","Kim C.J.","Ecker J.R."],"title":"Arabidopsis ORF clones.","publicationDate":"JUN-2008","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-3TH3AFLAVIRO9","citationType":"submission","authors":["Brover V.V.","Troukhan M.E.","Alexandrov N.A.","Lu Y.-P.","Flavell R.B.","Feldmann K.A."],"title":"Full-length cDNA from Arabidopsis thaliana.","publicationDate":"MAR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-336"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL035528","properties":[{"key":"ProteinId","value":"CAB36844.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL161537","properties":[{"key":"ProteinId","value":"CAB78422.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002687","properties":[{"key":"ProteinId","value":"AEE83326.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002687","properties":[{"key":"ProteinId","value":"ANM66023.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002687","properties":[{"key":"ProteinId","value":"ANM66025.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002687","properties":[{"key":"ProteinId","value":"ANM66026.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408686","properties":[{"key":"ProteinId","value":"CAO7047049.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BT033091","properties":[{"key":"ProteinId","value":"ACF04814.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY088039","properties":[{"key":"ProteinId","value":"AAM65585.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"T05249","properties":[{"key":"EntryName","value":"T05249"}]},{"database":"RefSeq","id":"NP_001319923.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001340877.1"}]},{"database":"RefSeq","id":"NP_001327951.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001340884.1"}]},{"database":"RefSeq","id":"NP_001327952.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001340881.1"}]},{"database":"RefSeq","id":"NP_567411.1","properties":[{"key":"NucleotideSequenceId","value":"NM_117454.2"}]},{"database":"AlphaFoldDB","id":"B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"B3LFA3","properties":[{"key":"Number of interactors","value":"3146"}]},{"database":"STRING","id":"3702.B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"249439","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"827014","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT4G13800","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT4G13800","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT4G13800","properties":[{"key":"GeneName","value":"ENOR3L3"}]},{"database":"eggNOG","id":"KOG2922","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_012349_1_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"MGAGEVC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:B3LFA3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 4"}]},{"database":"ExpressionAtlas","id":"B3LFA3","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005769","properties":[{"key":"GoTerm","value":"C:early endosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"HDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14506206"}]},{"database":"GO","id":"GO:0015095","properties":[{"key":"GoTerm","value":"F:magnesium ion transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0015693","properties":[{"key":"GoTerm","value":"P:magnesium ion transport"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"InterPro","id":"IPR037185","properties":[{"key":"EntryName","value":"EmrE-like"}]},{"database":"InterPro","id":"IPR008521","properties":[{"key":"EntryName","value":"Mg_trans_NIPA"}]},{"database":"PANTHER","id":"PTHR12570","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12570:SF67","properties":[{"key":"EntryName","value":"MAGNESIUM TRANSPORTER NIPA2-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05653","properties":[{"key":"EntryName","value":"Mg_trans_NIPA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103481","properties":[{"key":"EntryName","value":"Multidrug resistance efflux transporter EmrE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEEMSPDNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITMIVGEIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHMFGILGCVLCVVGSTTIVLHAPHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVYVGICSLMGSLTVMSVKAVAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNKALDNFNTAVISPVYYVMFTTFTILASMIMFKDWASQSGLQIATELCGFVTILSGTFLLHKTKDMGNSTSLRGSTSHSPRDTPVFINSGSSRSSNSTRPAIL","length":336,"molWeight":36600,"crc64":"A07AE7BBAF84B5FA","md5":"9097D82598F7EDCEA602AB53296E9321"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":2},"countByFeatureType":{"Chain":1,"Topological domain":10,"Transmembrane":9,"Region":1,"Compositional bias":1,"Sequence conflict":1},"uniParcId":"UPI0000162A2C"}}