{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"B1XI94","secondaryAccessions":["B0FYI2"],"uniProtkbId":"CPCT_PICP2","entryAudit":{"firstPublicDate":"2011-01-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-05-20","entryVersion":78,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Picosynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6)","commonName":"Agmenellum quadruplicatum","taxonId":32049,"lineage":["Bacteria","Bacillati","Cyanobacteriota","Cyanophyceae","Oscillatoriophycideae","Chroococcales","Geminocystaceae","Picosynechococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Phycocyanobilin lyase CpcT"},"ecNumbers":[{"value":"4.-.-.-"}]}},"genes":[{"geneName":{"value":"cpcT"},"orderedLocusNames":[{"value":"SYNPCC7002_A2095"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16644722"}],"value":"Attaches a phycocyanobilin (PCB) chromophore to 'Cys-153' of the C-phycocyanin beta subunit (PhcB). It does not attach chromophore to CpcA, ApcA or ApcB, the other phycobiliproteins"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16644722"}],"value":"Cells are a yellow color, 40% reduction in phycocyanin and grow slowly. The C-phycocyanin beta subunit (PhcB) in this strain is missing the phycocyanobilin chromophore on position 153"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CpcT/CpeT biliprotein lyase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"Phycocyanobilin lyase CpcT","featureId":"PRO_0000403163"},{"type":"Sequence conflict","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"in Ref. 1; ABY63662","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["P"]}}],"keywords":[{"id":"KW-0456","category":"Molecular function","name":"Lyase"}],"references":[{"referenceNumber":1,"citation":{"id":"16644722","citationType":"journal article","authors":["Shen G.","Saunee N.A.","Williams S.R.","Gallo E.F.","Schluchter W.M.","Bryant D.A."],"citationCrossReferences":[{"database":"PubMed","id":"16644722"},{"database":"DOI","id":"10.1074/jbc.m602563200"}],"title":"Identification and characterization of a new class of bilin lyase: the cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Synechococcus sp. PCC 7002.","publicationDate":"2006","journal":"J. Biol. Chem.","firstPage":"17768","lastPage":"17778","volume":"281"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION IN CHROMOPHORE ATTACHMENT","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"ATCC 27264 / PCC 7002 / PR-6","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"CI-26NA1B8V4FQH4","citationType":"submission","authors":["Li T.","Zhao J.","Zhao C.","Liu Z.","Zhao F.","Marquardt J.","Nomura C.T.","Persson S.","Detter J.C.","Richardson P.M.","Lanz C.","Schuster S.C.","Wang J.","Li S.","Huang X.","Cai T.","Yu Z.","Luo J.","Zhao J.","Bryant D.A."],"title":"Complete sequence of Synechococcus sp. PCC 7002.","publicationDate":"FEB-2008","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 27264 / PCC 7002 / PR-6","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"EU364770","properties":[{"key":"ProteinId","value":"ABY63662.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP000951","properties":[{"key":"ProteinId","value":"ACB00079.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_012307700.1","properties":[{"key":"NucleotideSequenceId","value":"NC_010475.1"}]},{"database":"AlphaFoldDB","id":"B1XI94","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"B1XI94","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"32049.SYNPCC7002_A2095","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"syp:SYNPCC7002_A2095","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502Z877","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_092589_0_0_3","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.4.1.30","properties":[{"key":"OrganismId","value":"6187"}]},{"database":"GO","id":"GO:0016829","properties":[{"key":"GoTerm","value":"F:lyase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0017009","properties":[{"key":"GoTerm","value":"P:protein-phycocyanobilin linkage"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16644722"}]},{"database":"CDD","id":"cd16338","properties":[{"key":"EntryName","value":"CpcT"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.128.590","properties":[{"key":"EntryName","value":"CpcT/CpeT domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01460","properties":[{"key":"EntryName","value":"Chrphore_lyase_CpxT"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR010404","properties":[{"key":"EntryName","value":"CpcT/CpeT"}]},{"database":"InterPro","id":"IPR038672","properties":[{"key":"EntryName","value":"CpcT/CpeT_sf"}]},{"database":"PANTHER","id":"PTHR35137","properties":[{"key":"EntryName","value":"CHROMOPHORE LYASE CRL, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35137:SF1","properties":[{"key":"EntryName","value":"CHROMOPHORE LYASE CRL, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06206","properties":[{"key":"EntryName","value":"CpeT"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSHSTDAHTLARWMAGEFSNEAQALANPPLWAHIKVCMRPLPNQFFDGYGLYLEQAYSSDTSAPYRLRLFHIKPVDDHMELVHYKPKDDAKTKYMGAARNPAMMQHFDMADLDPMPGCDMIVTWSGTSFKGTVQAGKGCRVVRYNKESYLDNSFEITDNALISIDRGRDPVTNEILWGSLAGAFEFEKINNFSGEVQPH","length":199,"molWeight":22483,"crc64":"AB3F5B6D35C343C4","md5":"E299B6AF783BBD024331AEF588D99096"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Sequence conflict":1},"uniParcId":"UPI00016DCC75"}}