{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"B1VB74","uniProtkbId":"PDUO_CITFR","entryAudit":{"firstPublicDate":"2022-02-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-05-20","entryVersion":53,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Citrobacter freundii","taxonId":546,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Citrobacter","Citrobacter freundii complex"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Corrinoid adenosyltransferase PduO"}},"alternativeNames":[{"fullName":{"value":"ATP:co(I)rrinoid adenosyltransferase PduO"},"shortNames":[{"value":"ACA"}]},{"fullName":{"value":"Propanediol utilization protein PduO"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"18332146"}],"value":"pduO"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8ZNR5"}],"value":"Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), the cofactor for propanediol dehydratase. 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PduO subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":335,"modifier":"EXACT"}},"description":"Corrinoid adenosyltransferase PduO","featureId":"PRO_0000454281"},{"type":"Binding site","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30413"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8ZNR5"}],"ligand":{"name":"heme","id":"ChEBI:CHEBI:30413"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-1283","category":"Cellular component","name":"Bacterial microcompartment"},{"id":"KW-0169","category":"Biological process","name":"Cobalamin biosynthesis"},{"id":"KW-0349","category":"Ligand","name":"Heme"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"18332146","citationType":"journal article","authors":["Parsons J.B.","Dinesh S.D.","Deery E.","Leech H.K.","Brindley A.A.","Heldt D.","Frank S.","Smales C.M.","Lunsdorf H.","Rambach A.","Gass M.H.","Bleloch A.","McClean K.J.","Munro A.W.","Rigby S.E.J.","Warren M.J.","Prentice M.B."],"citationCrossReferences":[{"database":"PubMed","id":"18332146"},{"database":"DOI","id":"10.1074/jbc.m709214200"}],"title":"Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis.","publicationDate":"2008","journal":"J. Biol. Chem.","firstPage":"14366","lastPage":"14375","volume":"283"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","FUNCTION","IDENTIFICATION BY MASS SPECTROMETRY","PATHWAY","SUBCELLULAR LOCATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AM498294","properties":[{"key":"ProteinId","value":"CAM57295.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"B1VB74","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"B1VB74","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00148","properties":[{"key":"RectionId","value":"-"}]},{"database":"UniPathway","id":"UPA00621","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0031469","properties":[{"key":"GoTerm","value":"C:bacterial microcompartment"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18332146"}]},{"database":"GO","id":"GO:0008817","properties":[{"key":"GoTerm","value":"F:corrinoid adenosyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:TreeGrafter"}]},{"database":"GO","id":"GO:0051144","properties":[{"key":"GoTerm","value":"P:1,2-propanediol catabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"GO","id":"GO:0009236","properties":[{"key":"GoTerm","value":"P:cobalamin biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"Gene3D","id":"1.20.1200.10","properties":[{"key":"EntryName","value":"Cobalamin adenosyltransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.450.150","properties":[{"key":"EntryName","value":"Haem-degrading domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR016030","properties":[{"key":"EntryName","value":"CblAdoTrfase-like"}]},{"database":"InterPro","id":"IPR036451","properties":[{"key":"EntryName","value":"CblAdoTrfase-like_sf"}]},{"database":"InterPro","id":"IPR009221","properties":[{"key":"EntryName","value":"PduO"}]},{"database":"InterPro","id":"IPR029499","properties":[{"key":"EntryName","value":"PduO-typ"}]},{"database":"InterPro","id":"IPR005624","properties":[{"key":"EntryName","value":"PduO/GlcC-like"}]},{"database":"InterPro","id":"IPR038084","properties":[{"key":"EntryName","value":"PduO/GlcC-like_sf"}]},{"database":"NCBIfam","id":"TIGR00636","properties":[{"key":"EntryName","value":"PduO_Nterm"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12213","properties":[{"key":"EntryName","value":"CORRINOID ADENOSYLTRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12213:SF0","properties":[{"key":"EntryName","value":"CORRINOID ADENOSYLTRANSFERASE MMAB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01923","properties":[{"key":"EntryName","value":"Cob_adeno_trans"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03928","properties":[{"key":"EntryName","value":"HbpS-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF036411","properties":[{"key":"EntryName","value":"ATR_PduO"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF89028","properties":[{"key":"EntryName","value":"Cobalamin adenosyltransferase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF143744","properties":[{"key":"EntryName","value":"GlcG-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAIYTRTGDAGTTALFTGQRVSKTHPRVEAYGTLDELNAALSLCVCAAKNPQHRQLLENIQLQLFWFSAELASESEQPAPEQRYISSEEIAALEAAIDTAMGRVPPLRSFILPGRSEAASRLHFARTLARRAERRLVELSTEISVRHVLMRYINRLSDCLYALARAEDHDAHQNNIIQKVAERYLAAIRTSATREPAMSLSFQELHQLTRAAVMRAEELQVPVVISIVDANGTQTVTWRMPDALLVSSELAPKKAWTAVAMKTATHELTSAVQPGAALYGLESHMQGKVVTFGGGYALWREGLLLGGLGISGGSVEQDMDIAETAIAAINVRTHQ","length":335,"molWeight":36699,"crc64":"9853FDF3C0F7923B","md5":"798E0FF1122AE0D9422A1750E6862842"},"extraAttributes":{"countByCommentType":{"FUNCTION":3,"CATALYTIC ACTIVITY":1,"COFACTOR":2,"PATHWAY":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":1},"uniParcId":"UPI0001717372"}}