{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"B0VHH0","uniProtkbId":"KCE_CLOAI","entryAudit":{"firstPublicDate":"2012-04-18","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-04-08","entryVersion":81,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Cloacimonas acidaminovorans (strain Evry)","taxonId":459349,"lineage":["Bacteria","Pseudomonadati","Candidatus Cloacimonadota","Candidatus Cloacimonadia","Candidatus Cloacimonadales","Candidatus Cloacimonadaceae","Candidatus Cloacimonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21632536"}],"value":"3-keto-5-aminohexanoate cleavage enzyme"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"value":"2.3.1.247"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"21632536"}],"value":"kce"},"orderedLocusNames":[{"value":"CLOAM0912"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"value":"Involved in the anaerobic fermentation of lysine. Catalyzes the reversible reaction between 3-keto-5-aminohexanoate (KAH) and acetyl-CoA to form 3-aminobutyryl-CoA and acetoacetate. The reaction involves the deprotonation of KAH, the nucleophilic addition onto acetyl-CoA and the intramolecular transfer of the CoA moiety"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(5S)-5-amino-3-oxohexanoate + acetyl-CoA = (3S)-3-aminobutanoyl-CoA + acetoacetate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:31555"},{"database":"ChEBI","id":"CHEBI:13705"},{"database":"ChEBI","id":"CHEBI:57288"},{"database":"ChEBI","id":"CHEBI:57366"},{"database":"ChEBI","id":"CHEBI:58523"}],"ecNumber":"2.3.1.247","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":0.208,"unit":"mM","substrate":"KAH","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}]},{"constant":0.012,"unit":"mM","substrate":"acetoacetate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"value":"kcat is 2.69 sec(-1) for 3-keto-5-aminohexanoate cleavage."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"21632536"}],"value":"Amino-acid degradation; L-lysine degradation via acetate pathway"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the BKACE family. Kce subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":276,"modifier":"EXACT"}},"description":"3-keto-5-aminohexanoate cleavage enzyme","featureId":"PRO_0000416975"},{"type":"Binding site","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":14,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58523"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"}],"ligand":{"name":"(5S)-5-amino-3-oxohexanoate","id":"ChEBI:CHEBI:58523"}},{"type":"Binding site","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7D"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7G"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7D"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7G"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Binding site","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58523"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"}],"ligand":{"name":"(5S)-5-amino-3-oxohexanoate","id":"ChEBI:CHEBI:58523"}},{"type":"Binding site","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58523"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"}],"ligand":{"name":"(5S)-5-amino-3-oxohexanoate","id":"ChEBI:CHEBI:58523"}},{"type":"Binding site","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58523"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"}],"ligand":{"name":"(5S)-5-amino-3-oxohexanoate","id":"ChEBI:CHEBI:58523"}},{"type":"Binding site","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58523"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"}],"ligand":{"name":"(5S)-5-amino-3-oxohexanoate","id":"ChEBI:CHEBI:58523"}},{"type":"Binding site","location":{"start":{"value":230,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7D"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7G"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105"}},{"type":"Mutagenesis","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"Reduced catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["G"]}},{"type":"Mutagenesis","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Reduced catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["G","Q"]}},{"type":"Mutagenesis","location":{"start":{"value":226,"modifier":"EXACT"},"end":{"value":226,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["G"]}},{"type":"Mutagenesis","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":231,"modifier":"EXACT"}},"description":"Loss of catalytic activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["G"]}},{"type":"Beta strand","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":9,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Turn","location":{"start":{"value":17,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":40,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":95,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":117,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":139,"modifier":"EXACT"},"end":{"value":144,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":158,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":168,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":206,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":208,"modifier":"EXACT"},"end":{"value":210,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Turn","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":228,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Turn","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":231,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Beta strand","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":236,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":244,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]},{"type":"Helix","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":271,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2Y7E"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"18245282","citationType":"journal article","authors":["Pelletier E.","Kreimeyer A.","Bocs S.","Rouy Z.","Gyapay G.","Chouari R.","Riviere D.","Ganesan A.","Daegelen P.","Sghir A.","Cohen G.N.","Medigue C.","Weissenbach J.","Le Paslier D."],"citationCrossReferences":[{"database":"PubMed","id":"18245282"},{"database":"DOI","id":"10.1128/jb.01248-07"}],"title":"'Candidatus Cloacamonas acidaminovorans': genome sequence reconstruction provides a first glimpse of a new bacterial division.","publicationDate":"2008","journal":"J. Bacteriol.","firstPage":"2572","lastPage":"2579","volume":"190"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Evry","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"21632536","citationType":"journal article","authors":["Bellinzoni M.","Bastard K.","Perret A.","Zaparucha A.","Perchat N.","Vergne C.","Wagner T.","de Melo-Minardi R.C.","Artiguenave F.","Cohen G.N.","Weissenbach J.","Salanoubat M.","Alzari P.M."],"citationCrossReferences":[{"database":"PubMed","id":"21632536"},{"database":"DOI","id":"10.1074/jbc.m111.253260"}],"title":"3-Keto-5-aminohexanoate cleavage enzyme: a common fold for an uncommon Claisen-type condensation.","publicationDate":"2011","journal":"J. Biol. Chem.","firstPage":"27399","lastPage":"27405","volume":"286"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.28 ANGSTROMS) OF 2-276 IN COMPLEXES WITH 5-AMINO-3-OXO-HEXANOATE; ACETOACETATE AND MG(2+)","FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","PATHWAY","MUTAGENESIS OF SER-82; GLU-143; ARG-226 AND ASP-231","REACTION MECHANISM","SUBUNIT"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7D"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7E"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7F"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"2Y7G"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CU466930","properties":[{"key":"ProteinId","value":"CAO80785.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_015424643.1","properties":[{"key":"NucleotideSequenceId","value":"NC_020449.1"}]},{"database":"PDB","id":"2Y7D","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.59 A"},{"key":"Chains","value":"A/B/C/D=2-276"}]},{"database":"PDB","id":"2Y7E","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.28 A"},{"key":"Chains","value":"A/B=2-276"}]},{"database":"PDB","id":"2Y7F","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.75 A"},{"key":"Chains","value":"A/B/C/D=2-276"}]},{"database":"PDB","id":"2Y7G","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.40 A"},{"key":"Chains","value":"A/B=2-276"}]},{"database":"PDBsum","id":"2Y7D","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2Y7E","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2Y7F","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2Y7G","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"B0VHH0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"B0VHH0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"459349.CLOAM0912","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"caci:CLOAM0912","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG3246","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_065536_2_0_0","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9801076at2","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.3.1.247","properties":[{"key":"OrganismId","value":"14207"}]},{"database":"UniPathway","id":"UPA00870","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"B0VHH0","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002019","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0043720","properties":[{"key":"GoTerm","value":"F:3-keto-5-aminohexanoate cleavage activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21632536"}]},{"database":"GO","id":"GO:0019475","properties":[{"key":"GoTerm","value":"P:L-lysine fermentation"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniPathway"}]},{"database":"Gene3D","id":"3.20.20.70","properties":[{"key":"EntryName","value":"Aldolase class I"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR013785","properties":[{"key":"EntryName","value":"Aldolase_TIM"}]},{"database":"InterPro","id":"IPR008567","properties":[{"key":"EntryName","value":"BKACE"}]},{"database":"InterPro","id":"IPR058240","properties":[{"key":"EntryName","value":"rSAM_sf"}]},{"database":"PANTHER","id":"PTHR37418:SF2","properties":[{"key":"EntryName","value":"3-KETO-5-AMINOHEXANOATE CLEAVAGE ENZYME"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37418","properties":[{"key":"EntryName","value":"3-KETO-5-AMINOHEXANOATE CLEAVAGE ENZYME-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05853","properties":[{"key":"EntryName","value":"BKACE"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF102114","properties":[{"key":"EntryName","value":"Radical SAM enzymes"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEPLILTAAITGAETTRADQPNLPITPEEQAKEAKACFEAGARVIHLHIREDDGRPSQRLDRFQEAISAIREVVPEIIIQISTGGAVGESFDKRLAPLALKPEMATLNAGTLNFGDDIFINHPADIIRLAEAFKQYNVVPEVEVYESGMVDAVARLIKKGIITQNPLHIQFVLGVPGGMSGKPKNLMYMMEHLKEEIPTATWAVAGIGRWHIPTSLIAMVTGGHIRCGFEDNIFYHKGVIAESNAQLVARLARIAKEIGRPLATPEQAREILALNK","length":276,"molWeight":30222,"crc64":"524B0E27FFC0FA5D","md5":"AFBF2E9FC1C85DBE1C5221F11DDFA47F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":8,"Mutagenesis":4,"Beta strand":11,"Turn":3,"Helix":11},"uniParcId":"UPI0000ECF250"}}