{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"B0B815","secondaryAccessions":["P18586","P21354"],"uniProtkbId":"OMCB_CHLT2","entryAudit":{"firstPublicDate":"2012-06-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2012-06-13","entryVersion":75,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Chlamydia trachomatis serovar L2 (strain ATCC VR-902B / DSM 19102 / 434/Bu)","taxonId":471472,"lineage":["Bacteria","Pseudomonadati","Chlamydiota","Chlamydiia","Chlamydiales","Chlamydiaceae","Chlamydia/Chlamydophila group","Chlamydia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Large cysteine-rich periplasmic protein OmcB"},"shortNames":[{"value":"Large-CRP"}]},"alternativeNames":[{"fullName":{"value":"60 kDa CRP"}},{"fullName":{"value":"60 kDa outer membrane protein"}},{"fullName":{"value":"Cysteine-rich outer membrane protein"}}],"contains":[{"recommendedName":{"fullName":{"value":"Large cysteine-rich periplasmic protein OmcB, isoform 1"}}},{"recommendedName":{"fullName":{"value":"Large cysteine-rich periplasmic protein OmcB, isoform 2"}}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"omcB"},"synonyms":[{"value":"omp2"},{"value":"omp2B"}],"orderedLocusNames":[{"value":"CTL0702"}]}],"comments":[{"texts":[{"value":"In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan"}],"commentType":"FUNCTION"},{"texts":[{"value":"Part of a disulfide cross-linked outer membrane complex (COMC) composed of the major outer membrane porin (MOMP), the small cysteine-rich protein (OmcA) and the large cysteine-rich periplasmic protein (OmcB)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Periplasm","id":"SL-0200"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2914847"}],"value":"It is present but the disulfide bonds are reduced in the intracellular reticulate bodies (RBs)"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"value":"In strain L2 the origin of the observed doublet has been shown to be different post-translational cleavage"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Was thought to be an outer membrane protein as it is part of a disulfide cross-linked complex that is insoluble in the detergent Sarkosyl; however based on experiments in C.psittaci it is likely to be periplasmic"}],"commentType":"CAUTION"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"CAP04141.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"2914847"}]},{"type":"Chain","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"Large cysteine-rich periplasmic protein OmcB, isoform 1","featureId":"PRO_0000417577"},{"type":"Chain","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":547,"modifier":"EXACT"}},"description":"Large cysteine-rich periplasmic protein OmcB, isoform 2","featureId":"PRO_0000417578"},{"type":"Region","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]}],"keywords":[{"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0574","category":"Cellular component","name":"Periplasm"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"2914847","citationType":"journal article","authors":["Allen J.E.","Stephens R.S."],"citationCrossReferences":[{"database":"PubMed","id":"2914847"},{"database":"DOI","id":"10.1128/jb.171.1.285-291.1989"}],"title":"Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2.","publicationDate":"1989","journal":"J. Bacteriol.","firstPage":"285","lastPage":"291","volume":"171"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]","PROTEIN SEQUENCE OF 23-32 AND 41-51","DEVELOPMENTAL STAGE","EXISTENCE OF TWO MATURE FORMS"],"referenceComments":[{"value":"ATCC VR-902B / DSM 19102 / 434/Bu","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"18032721","citationType":"journal article","authors":["Thomson N.R.","Holden M.T.G.","Carder C.","Lennard N.","Lockey S.J.","Marsh P.","Skipp P.","O'Connor C.D.","Goodhead I.","Norbertzcak H.","Harris B.","Ormond D.","Rance R.","Quail M.A.","Parkhill J.","Stephens R.S.","Clarke I.N."],"citationCrossReferences":[{"database":"PubMed","id":"18032721"},{"database":"DOI","id":"10.1101/gr.7020108"}],"title":"Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolates.","publicationDate":"2008","journal":"Genome Res.","firstPage":"161","lastPage":"171","volume":"18"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC VR-902B / DSM 19102 / 434/Bu","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"M23001","properties":[{"key":"ProteinId","value":"AAA23152.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AM884176","properties":[{"key":"ProteinId","value":"CAP04141.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A32244","properties":[{"key":"EntryName","value":"A32244"}]},{"database":"AlphaFoldDB","id":"B0B815","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"B0B815","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"1.B.2.1.2","properties":[{"key":"FamilyName","value":"the chlamydial porin (cp) family"}]},{"database":"KEGG","id":"ctb:CTL0702","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|471472.4.peg.754","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"HOGENOM","id":"CLU_029611_0_0_0","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0042597","properties":[{"key":"GoTerm","value":"C:periplasmic space"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005201","properties":[{"key":"GoTerm","value":"F:extracellular matrix structural constituent"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003506","properties":[{"key":"EntryName","value":"Chlam_OMP6"}]},{"database":"InterPro","id":"IPR051172","properties":[{"key":"EntryName","value":"Chlamydia_OmcB"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR001434","properties":[{"key":"EntryName","value":"OmcB-like_DUF11"}]},{"database":"PANTHER","id":"PTHR34819","properties":[{"key":"EntryName","value":"LARGE CYSTEINE-RICH PERIPLASMIC PROTEIN OMCB"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34819:SF4","properties":[{"key":"EntryName","value":"LARGE CYSTEINE-RICH PERIPLASMIC PROTEIN OMCB"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03504","properties":[{"key":"EntryName","value":"Chlam_OMP6"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01345","properties":[{"key":"EntryName","value":"CLIPPER"},{"key":"MatchStatus","value":"3"}]},{"database":"PRINTS","id":"PR01336","properties":[{"key":"EntryName","value":"CHLAMIDIAOM6"}]}],"sequence":{"value":"MNKLIRRAVTIFAVTSVASLFASGVLETSMAEFISTNVISLADTKAKDNTSHKSKKARKNHSKETPVNRKKVAPVHESKATGPKQDSCFGRMYTVKVNDDRNVEITQAVPKYATVGSPYPVEITATGKRDCVDVIITQQLPCEAEFVRSDPATTPTADGKLVWKIDRLGQGEKSKITVWVKPLKEGCCFTAATVCACPEIRSVTKCGQPAICVKQEGPENACLRCPVVYKINVVNQGTATARNVVVENPVPDSYAHSSGQRVLTFTLGDMQPGEHRTITVEFCPLKRGRATNIAMVSYCGGHKNTASVTTVINEPCVQVSIAGADWSYVCKPVEYVISVSNPGDLVLRDVVVKDTLSPGVTVLEAAGAQISCNKVVWTVKELNPGESLQYKVLVRAQTPGQFTNNVVVKSCSDCGTCTSCAEATTYWKGVAATHMCVVDTCDPVCVGENTVYRICVTNRGSAEDTNVSLMLKFSKELQPVSFSGPTKGTITGNTVVFDSLPRLGSKETVEFSVTLKAVSAGDARGEAILSSDTLTVPVSDTENTHIY","length":547,"molWeight":58782,"crc64":"78CEB41CCF98472D","md5":"36B06B9C494EAFB7C1EB503D61DA718A"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DEVELOPMENTAL STAGE":1,"MISCELLANEOUS":1,"CAUTION":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Signal":1,"Chain":2,"Region":1,"Compositional bias":1},"uniParcId":"UPI0000130CC8"}}