{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A8K0Z3","uniProtkbId":"WASH1_HUMAN","entryAudit":{"firstPublicDate":"2008-04-08","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2010-01-19","entryVersion":134,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:24361"}],"value":"WASH complex subunit 1"}},"alternativeNames":[{"fullName":{"value":"CXYorf1-like protein on chromosome 9"}},{"fullName":{"value":"Protein FAM39E"}},{"fullName":{"value":"WAS protein family homolog 1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:24361"}],"value":"WASHC1"},"synonyms":[{"value":"FAM39E"},{"value":"WASH1"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C4AMC7"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8VDD8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20498093"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22114305"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23452853"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"20498093"}],"value":"Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922874, PubMed:19922875, PubMed:20498093, PubMed:23452853). Involved in endocytic trafficking of EGF (By similarity). Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration (PubMed:22114305). In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation (By similarity). Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex (PubMed:24344185). Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"41073792"}],"value":"(Microbial infection) Nucleation-promoting factor that plays a major role in human papillomavirus infection by facilitating branched actin-driven scission in endocytosis"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8VDD8"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4E1"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20498093"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24344185"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29084197"}],"value":"Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH (WASHC1, WASH2P or WASH3P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. The WASH core complex associates via WASHC2 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex (PubMed:19922875, PubMed:20498093). Interacts (via WHD1 region) with WASHC2C; the interaction is direct (PubMed:19922874). Interacts with VPS35; mediates the association with the retromer CSC complex. Interacts with FKBP15. Interacts with alpha-tubulin. Interacts with BECN1; this interaction can be competed out by AMBRA1 binding. Interacts with BLOC1S2; may associate with the BLOC-1 complex. Interacts with tubulin gamma chain (TUBG1 or TUBG2) (By similarity). Interacts with EXOC1, EXOC4, EXOC8; in MMP14-positive endosomes in breast tumor cells; indicative for an association with the exocyst complex (PubMed:24344185). Interacts with TBC1D23 (PubMed:29084197)"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"A8K0Z3","intActId":"EBI-6160405"},"interactantTwo":{"uniProtKBAccession":"Q14457","geneName":"BECN1","intActId":"EBI-949378"},"numberOfExperiments":3,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"A8K0Z3","intActId":"EBI-6160405"},"interactantTwo":{"uniProtKBAccession":"Q9NV70","geneName":"EXOC1","intActId":"EBI-1045313"},"numberOfExperiments":2,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"A8K0Z3","intActId":"EBI-6160405"},"interactantTwo":{"uniProtKBAccession":"Q8IYI6","geneName":"EXOC8","intActId":"EBI-742102"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8VDD8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24344185"}],"value":"Localization to the endosome membrane is mediated via its interaction with WASHC2 (PubMed:19922874). Localizes to MMP14-positive late endosomes and transiently to invadipodia (PubMed:24344185). Localized to Salmonella typhimurium entry sites (By similarity)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922874"}],"value":"Early endosome membrane","id":"SL-0093"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8VDD8"}],"value":"Recycling endosome membrane","id":"SL-0231"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22114305"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24344185"}],"value":"Late endosome","id":"SL-0152"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8VDD8"}],"value":"Cytoplasmic vesicle, autophagosome","id":"SL-0023"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8VDD8"}],"value":"Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole","id":"SL-0046"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C4AMC7"}],"value":"The VCA (verprolin, cofilin, acidic) domain promotes actin polymerization by the Arp2/3 complex in vitro"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23452853"}],"value":"Ubiquitinated at Lys-220 via 'Lys-63'-linked ubiquitin chains by the TRIM27:MAGEL2 E3 ubiquitin ligase complex, leading to promote endosomal F-actin assembly"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18159949"}],"value":"WASH genes duplicated to multiple chromosomal ends during primate evolution, with highest copy number reached in humans, whose WASH repertoires probably vary extensively among individuals (PubMed:18159949). It is therefore difficult to determine which gene is functional or not. The telomeric region of chromosome 9p is paralogous to the pericentromeric regions of chromosome 9 as well as to 2q. Paralogous regions contain 7 transcriptional units. Duplicated WASH genes are also present in the Xq/Yq pseudoautosomal region, as well as on chromosome 1 and 15. The chromosome 16 copy seems to be a pseudogene"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the WASH1 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20498093"}],"value":"One study reported a nucleation-promoting factor (NPF) activity towards the Arp2/3 complex using partially purified samples of the WASH complex (PubMed:19922875). In another study, the in vitro reconstituted and purified recombinant WASH core complex, consisting of WASHC3, WASHC4, WASHC5, WASHC1 and the N-terminal residues 1-356 of WASHC2, did not show any NPF activity towards the Arp2/3 complex (PubMed:20498093)"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"WASH complex subunit 1","featureId":"PRO_0000329013"},{"type":"Domain","location":{"start":{"value":361,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"WH2"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"WHD1"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Required for WASH complex assembly","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C4AMC7"}]},{"type":"Region","location":{"start":{"value":297,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"VCA","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"C4AMC7"}]},{"type":"Region","location":{"start":{"value":376,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":423,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":302,"modifier":"EXACT"},"end":{"value":314,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":398,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":424,"modifier":"EXACT"},"end":{"value":436,"modifier":"EXACT"}},"description":"Gly residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":456,"modifier":"EXACT"},"end":{"value":465,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Cross-link","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23452853"}]},{"type":"Mutagenesis","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Abolishes ubiquitination by the TRIM27:MAGEL2 E3 ubiquitin ligase complex and impairs retrograde transport.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23452853"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":463,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"Impairs retrograde transport from endosome to Golgi apparatus.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19922874"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":19,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["L"]}},{"type":"Sequence conflict","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"Q","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":98,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["E"]}},{"type":"Sequence conflict","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":119,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":141,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["Y"]}},{"type":"Sequence conflict","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":245,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":294,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":302,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["TAPP"]}},{"type":"Sequence conflict","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["S"]}},{"type":"Sequence conflict","location":{"start":{"value":340,"modifier":"EXACT"},"end":{"value":342,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"SSS","alternativeSequences":["GSN"]}},{"type":"Sequence conflict","location":{"start":{"value":362,"modifier":"EXACT"},"end":{"value":362,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["V"]}},{"type":"Sequence conflict","location":{"start":{"value":391,"modifier":"EXACT"},"end":{"value":392,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"QQ","alternativeSequences":["KK"]}},{"type":"Sequence conflict","location":{"start":{"value":407,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["D"]}},{"type":"Sequence conflict","location":{"start":{"value":439,"modifier":"EXACT"},"end":{"value":439,"modifier":"EXACT"}},"description":"in Ref. 1; BAF82397","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"V","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0009","category":"Molecular function","name":"Actin-binding"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"id":"KW-0206","category":"Cellular component","name":"Cytoskeleton"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-1017","category":"PTM","name":"Isopeptide bond"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"}],"references":[{"referenceNumber":1,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Brain","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15164053","citationType":"journal article","authors":["Humphray S.J.","Oliver K.","Hunt A.R.","Plumb R.W.","Loveland J.E.","Howe K.L.","Andrews T.D.","Searle S.","Hunt S.E.","Scott C.E.","Jones M.C.","Ainscough R.","Almeida J.P.","Ambrose K.D.","Ashwell R.I.S.","Babbage A.K.","Babbage S.","Bagguley C.L.","Bailey J.","Banerjee R.","Barker D.J.","Barlow K.F.","Bates K.","Beasley H.","Beasley O.","Bird C.P.","Bray-Allen S.","Brown A.J.","Brown J.Y.","Burford D.","Burrill W.","Burton J.","Carder C.","Carter N.P.","Chapman J.C.","Chen Y.","Clarke G.","Clark S.Y.","Clee C.M.","Clegg S.","Collier R.E.","Corby N.","Crosier M.","Cummings A.T.","Davies J.","Dhami P.","Dunn M.","Dutta I.","Dyer L.W.","Earthrowl M.E.","Faulkner L.","Fleming C.J.","Frankish A.","Frankland J.A.","French L.","Fricker D.G.","Garner P.","Garnett J.","Ghori J.","Gilbert J.G.R.","Glison C.","Grafham D.V.","Gribble S.","Griffiths C.","Griffiths-Jones S.","Grocock R.","Guy J.","Hall R.E.","Hammond S.","Harley J.L.","Harrison E.S.I.","Hart E.A.","Heath P.D.","Henderson C.D.","Hopkins B.L.","Howard P.J.","Howden P.J.","Huckle E.","Johnson C.","Johnson D.","Joy A.A.","Kay M.","Keenan S.","Kershaw J.K.","Kimberley A.M.","King A.","Knights A.","Laird G.K.","Langford C.","Lawlor S.","Leongamornlert D.A.","Leversha M.","Lloyd C.","Lloyd D.M.","Lovell J.","Martin S.","Mashreghi-Mohammadi M.","Matthews L.","McLaren S.","McLay K.E.","McMurray A.","Milne S.","Nickerson T.","Nisbett J.","Nordsiek G.","Pearce A.V.","Peck A.I.","Porter K.M.","Pandian R.","Pelan S.","Phillimore B.","Povey S.","Ramsey Y.","Rand V.","Scharfe M.","Sehra H.K.","Shownkeen R.","Sims S.K.","Skuce C.D.","Smith M.","Steward C.A.","Swarbreck D.","Sycamore N.","Tester J.","Thorpe A.","Tracey A.","Tromans A.","Thomas D.W.","Wall M.","Wallis J.M.","West A.P.","Whitehead S.L.","Willey D.L.","Williams S.A.","Wilming L.","Wray P.W.","Young L.","Ashurst J.L.","Coulson A.","Blocker H.","Durbin R.M.","Sulston J.E.","Hubbard T.","Jackson M.J.","Bentley D.R.","Beck S.","Rogers J.","Dunham I."],"citationCrossReferences":[{"database":"PubMed","id":"15164053"},{"database":"DOI","id":"10.1038/nature02465"}],"title":"DNA sequence and analysis of human chromosome 9.","publicationDate":"2004","journal":"Nature","firstPage":"369","lastPage":"374","volume":"429"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":3,"citation":{"id":"10655549","citationType":"journal article","authors":["Ciccodicola A.","D'Esposito M.","Esposito T.","Gianfrancesco F.","Migliaccio C.","Miano M.G.","Matarazzo M.R.","Vacca M.","Franze A.","Cuccurese M.","Cocchia M.","Curci A.","Terracciano A.","Torino A.","Cocchia S.","Mercadante G.","Pannone E.","Archidiacono N.","Rocchi M.","Schlessinger D.","D'Urso M."],"citationCrossReferences":[{"database":"PubMed","id":"10655549"},{"database":"DOI","id":"10.1093/hmg/9.3.395"}],"title":"Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region.","publicationDate":"2000","journal":"Hum. Mol. Genet.","firstPage":"395","lastPage":"401","volume":"9"},"referencePositions":["GENE DUPLICATION"]},{"referenceNumber":4,"citation":{"id":"18159949","citationType":"journal article","authors":["Linardopoulou E.V.","Parghi S.S.","Friedman C.","Osborn G.E.","Parkhurst S.M.","Trask B.J."],"citationCrossReferences":[{"database":"PubMed","id":"18159949"},{"database":"DOI","id":"10.1371/journal.pgen.0030237"}],"title":"Human subtelomeric WASH genes encode a new subclass of the WASP family.","publicationDate":"2007","journal":"PLoS Genet.","firstPage":"E237","lastPage":"E237","volume":"3"},"referencePositions":["GENE DUPLICATION"]},{"referenceNumber":5,"citation":{"id":"19922874","citationType":"journal article","authors":["Gomez T.S.","Billadeau D.D."],"citationCrossReferences":[{"database":"PubMed","id":"19922874"},{"database":"DOI","id":"10.1016/j.devcel.2009.09.009"}],"title":"A FAM21-containing WASH complex regulates retromer-dependent sorting.","publicationDate":"2009","journal":"Dev. Cell","firstPage":"699","lastPage":"711","volume":"17"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH WASHC2C","TUBULIN-BINDING","MUTAGENESIS OF TRP-463"]},{"referenceNumber":6,"citation":{"id":"19922875","citationType":"journal article","authors":["Derivery E.","Sousa C.","Gautier J.J.","Lombard B.","Loew D.","Gautreau A."],"citationCrossReferences":[{"database":"PubMed","id":"19922875"},{"database":"DOI","id":"10.1016/j.devcel.2009.09.010"}],"title":"The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex.","publicationDate":"2009","journal":"Dev. Cell","firstPage":"712","lastPage":"723","volume":"17"},"referencePositions":["FUNCTION","FUNCTION OF THE WASH COMPLEX","IDENTIFICATION IN THE WASH COMPLEX"]},{"referenceNumber":7,"citation":{"id":"20498093","citationType":"journal article","authors":["Jia D.","Gomez T.S.","Metlagel Z.","Umetani J.","Otwinowski Z.","Rosen M.K.","Billadeau D.D."],"citationCrossReferences":[{"database":"PubMed","id":"20498093"},{"database":"DOI","id":"10.1073/pnas.0913293107"}],"title":"WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes.","publicationDate":"2010","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"10442","lastPage":"10447","volume":"107"},"referencePositions":["IDENTIFICATION IN THE WASH CORE COMPLEX"]},{"referenceNumber":8,"citation":{"id":"22114305","citationType":"journal article","authors":["Zech T.","Calaminus S.D.","Caswell P.","Spence H.J.","Carnell M.","Insall R.H.","Norman J.","Machesky L.M."],"citationCrossReferences":[{"database":"PubMed","id":"22114305"},{"database":"DOI","id":"10.1242/jcs.080986"}],"title":"The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated invasive migration.","publicationDate":"2011","journal":"J. Cell Sci.","firstPage":"3753","lastPage":"3759","volume":"124"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":9,"citation":{"id":"23452853","citationType":"journal article","authors":["Hao Y.H.","Doyle J.M.","Ramanathan S.","Gomez T.S.","Jia D.","Xu M.","Chen Z.J.","Billadeau D.D.","Rosen M.K.","Potts P.R."],"citationCrossReferences":[{"database":"PubMed","id":"23452853"},{"database":"DOI","id":"10.1016/j.cell.2013.01.051"}],"title":"Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination.","publicationDate":"2013","journal":"Cell","firstPage":"1051","lastPage":"1064","volume":"152"},"referencePositions":["FUNCTION","UBIQUITINATION AT LYS-220","MUTAGENESIS OF LYS-220"]},{"referenceNumber":10,"citation":{"id":"24344185","citationType":"journal article","authors":["Monteiro P.","Rosse C.","Castro-Castro A.","Irondelle M.","Lagoutte E.","Paul-Gilloteaux P.","Desnos C.","Formstecher E.","Darchen F.","Perrais D.","Gautreau A.","Hertzog M.","Chavrier P."],"citationCrossReferences":[{"database":"PubMed","id":"24344185"},{"database":"DOI","id":"10.1083/jcb.201306162"}],"title":"Endosomal WASH and exocyst complexes control exocytosis of MT1-MMP at invadopodia.","publicationDate":"2013","journal":"J. Cell Biol.","firstPage":"1063","lastPage":"1079","volume":"203"},"referencePositions":["FUNCTION","INTERACTION WITH EXOC1; EXOC4 AND EXOC8","SUBCELLULAR LOCATION"]},{"referenceNumber":11,"citation":{"id":"29084197","citationType":"journal article","authors":["Shin J.J.H.","Gillingham A.K.","Begum F.","Chadwick J.","Munro S."],"citationCrossReferences":[{"database":"PubMed","id":"29084197"},{"database":"DOI","id":"10.1038/ncb3627"}],"title":"TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golgi.","publicationDate":"2017","journal":"Nat. Cell Biol.","firstPage":"1424","lastPage":"1432","volume":"19"},"referencePositions":["INTERACTION WITH TBC1D23"]},{"referenceNumber":12,"citation":{"id":"41073792","citationType":"journal article","authors":["Brinkert P.","Krebs L.","Samperio Ventayol P.","Greune L.","Bannach C.","Amakiri C.","Bucher D.","Kollasser J.","Dersch P.","Boulant S.","Stradal T.E.B.","Schelhaas M."],"citationCrossReferences":[{"database":"PubMed","id":"41073792"},{"database":"DOI","id":"10.1038/s44319-025-00594-3"}],"title":"The actin nucleation promoting factor WASH facilitates clathrin-independent endocytosis of human papillomaviruses.","publicationDate":"2025","journal":"EMBO Rep.","firstPage":"5533","lastPage":"5566","volume":"26"},"referencePositions":["FUNCTION (MICROBIAL INFECTION)"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK289708","properties":[{"key":"ProteinId","value":"BAF82397.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AL928970","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"CCDS","id":"CCDS78375.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001365019.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001378090.1"}]},{"database":"RefSeq","id":"NP_878908.4","properties":[{"key":"NucleotideSequenceId","value":"NM_182905.6"}]},{"database":"RefSeq","id":"XP_024303137.1","properties":[{"key":"NucleotideSequenceId","value":"XM_024447369.2"}]},{"database":"RefSeq","id":"XP_047278535.1","properties":[{"key":"NucleotideSequenceId","value":"XM_047422579.1"}]},{"database":"AlphaFoldDB","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"938833","properties":[{"key":"Interactions","value":"90"}]},{"database":"ComplexPortal","id":"CPX-1163","properties":[{"key":"EntryName","value":"WASH complex, variant WASHC1/WASHC2C"}]},{"database":"ComplexPortal","id":"CPX-1172","properties":[{"key":"EntryName","value":"WASH complex, variant WASHC1/WASHC2A"}]},{"database":"CORUM","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"A8K0Z3","properties":[{"key":"Number of interactors","value":"360"}]},{"database":"IntAct","id":"A8K0Z3","properties":[{"key":"Interactions","value":"26"}]},{"database":"MINT","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"NDEx","id":"MUSIC2-C5225-WASHC1","properties":[{"key":"Evidences","value":"Osteosarcoma (U2OS) cell map - Endosomal organization and transportation assembly 2 (24 proteins)"}]},{"database":"STRING","id":"9606.ENSP00000485627","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"9.A.3.1.2","properties":[{"key":"FamilyName","value":"the sorting nexin27 (snx27)-retromer assembly apparatus (retromeraa) family"}]},{"database":"iPTMnet","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"MetOSite","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"WASHC1","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000485627","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"1843","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"75837","properties":[{"key":"antibodies","value":"10 antibodies from 9 providers"}]},{"database":"DNASU","id":"100287171","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000442898.5","properties":[{"key":"ProteinId","value":"ENSP00000485627.1"},{"key":"GeneId","value":"ENSG00000181404.18"}]},{"database":"Ensembl","id":"ENST00000696149.1","properties":[{"key":"ProteinId","value":"ENSP00000512441.1"},{"key":"GeneId","value":"ENSG00000181404.18"}]},{"database":"GeneID","id":"100287171","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:100287171","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000696149.1","properties":[{"key":"ProteinId","value":"ENSP00000512441.1"},{"key":"RefSeqNucleotideId","value":"NM_001378090.1"},{"key":"RefSeqProteinId","value":"NP_001365019.1"}]},{"database":"UCSC","id":"uc010mgm.2","properties":[{"key":"OrganismName","value":"human"}]},{"database":"AGR","id":"HGNC:24361","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA162409121","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"100287171","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"100287171","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"WASHC1","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:24361","properties":[{"key":"GeneName","value":"WASHC1"}]},{"database":"HPA","id":"ENSG00000181404","properties":[{"key":"ExpressionPatterns","value":"Low tissue specificity"}]},{"database":"MIM","id":"613632","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000181404","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000181404","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG1366","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000016717","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_029156_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"EKGNRWS","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9535213at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"A8K0Z3","properties":[{"key":"Number of GO annotations","value":"7 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"SignaLink","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"SIGNOR","id":"A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000181404","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"100287171","properties":[{"key":"hits","value":"61 hits in 233 CRISPR screens"}]},{"database":"ChiTaRS","id":"WASHC1","properties":[{"key":"OrganismName","value":"human"}]},{"database":"GenomeRNAi","id":"100287171","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"A8K0Z3","properties":[{"key":"DevelopmentLevel","value":"Tbio"}]},{"database":"PRO","id":"PR:A8K0Z3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 9"}]},{"database":"RNAct","id":"A8K0Z3","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000181404","properties":[{"key":"ExpressionPatterns","value":"Expressed in left uterine tube and 100 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"A8K0Z3","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005776","properties":[{"key":"GoTerm","value":"C:autophagosome"},{"key":"GoEvidenceType","value":"ISS:ParkinsonsUK-UCL"}]},{"database":"GO","id":"GO:0005814","properties":[{"key":"GoTerm","value":"C:centriole"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:GOC"}]},{"database":"GO","id":"GO:0005769","properties":[{"key":"GoTerm","value":"C:early endosome"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19922874"}]},{"database":"GO","id":"GO:0031901","properties":[{"key":"GoTerm","value":"C:early endosome membrane"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19922874"}]},{"database":"GO","id":"GO:0005770","properties":[{"key":"GoTerm","value":"C:late endosome"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22114305"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24344185"}]},{"database":"GO","id":"GO:0055037","properties":[{"key":"GoTerm","value":"C:recycling endosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0055038","properties":[{"key":"GoTerm","value":"C:recycling endosome membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0071203","properties":[{"key":"GoTerm","value":"C:WASH complex"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19922875"}]},{"database":"GO","id":"GO:0043014","properties":[{"key":"GoTerm","value":"F:alpha-tubulin binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"19922874"}]},{"database":"GO","id":"GO:0141039","properties":[{"key":"GoTerm","value":"F:phosphatidylinositol 3-kinase inhibitor activity"},{"key":"GoEvidenceType","value":"ISS:ParkinsonsUK-UCL"}]},{"database":"GO","id":"GO:0031625","properties":[{"key":"GoTerm","value":"F:ubiquitin protein ligase binding"},{"key":"GoEvidenceType","value":"IPI:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"23452853"}]},{"database":"GO","id":"GO:0034314","properties":[{"key":"GoTerm","value":"P:Arp2/3 complex-mediated actin nucleation"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23452853"}]},{"database":"GO","id":"GO:0032456","properties":[{"key":"GoTerm","value":"P:endocytic recycling"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22114305"}]},{"database":"GO","id":"GO:0016197","properties":[{"key":"GoTerm","value":"P:endosomal transport"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0006887","properties":[{"key":"GoTerm","value":"P:exocytosis"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24344185"}]},{"database":"GO","id":"GO:0022617","properties":[{"key":"GoTerm","value":"P:extracellular matrix disassembly"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24344185"}]},{"database":"GO","id":"GO:0010507","properties":[{"key":"GoTerm","value":"P:negative regulation of autophagy"},{"key":"GoEvidenceType","value":"ISS:ParkinsonsUK-UCL"}]},{"database":"GO","id":"GO:0030335","properties":[{"key":"GoTerm","value":"P:positive regulation of cell migration"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22114305"}]},{"database":"GO","id":"GO:0031274","properties":[{"key":"GoTerm","value":"P:positive regulation of pseudopodium assembly"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22114305"}]},{"database":"GO","id":"GO:0034315","properties":[{"key":"GoTerm","value":"P:regulation of Arp2/3 complex-mediated actin nucleation"},{"key":"GoEvidenceType","value":"NAS:ComplexPortal"}],"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"19922875"}]},{"database":"GO","id":"GO:0031396","properties":[{"key":"GoTerm","value":"P:regulation of protein ubiquitination"},{"key":"GoEvidenceType","value":"ISS:ParkinsonsUK-UCL"}]},{"database":"GO","id":"GO:0042147","properties":[{"key":"GoTerm","value":"P:retrograde transport, endosome to Golgi"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23452853"}]},{"database":"InterPro","id":"IPR028290","properties":[{"key":"EntryName","value":"WASH1"}]},{"database":"InterPro","id":"IPR021854","properties":[{"key":"EntryName","value":"WASH1_WAHD"}]},{"database":"PANTHER","id":"PTHR23331","properties":[{"key":"EntryName","value":"CXYORF1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR23331:SF5","properties":[{"key":"EntryName","value":"WAS PROTEIN FAMILY HOMOLOG 2-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11945","properties":[{"key":"EntryName","value":"WASH_WAHD"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTPVRMQHSLAGQTYAVPFIQPDLRREEAVQQMADALQYLQKVSGDIFSRISQQVEQSRSQVQAIGEKVSLAQAKIEKIKGSKKAIKVFSSAKYPAPGRLQEYGSIFTGAQDPGLQRRPRHRIQSKHRPLDERALQEKLKDFPVCVSTKPEPEDDAEEGLGGLPSNISSVSSLLLFNTTENLYKKYVFLDPLAGAVTKTHVMLGAETEEKLFDAPLSISKREQLEQQVPENYFYVPDLGQVPEIHVPSYLPDLPGIANDLMYSADLGPGIAPSAPGTIPELPTFHTEVAEPLKVDLQDGVLTPPPPPPPPPPAPEVLASAPPLPPSTAAPVGQGARQDDSSSSASPSVQGAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKQQQKEQEQVRATSQGGHLMSDLFNKLVMRRKGISGKGPGAGEGPGGAFVRVSDSIPPLPPPQQPQAEEDEDDWES","length":465,"molWeight":50328,"crc64":"DBD5DEAFB7019B3E","md5":"6C74F01BA06996A7891528D611DE1838"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"SUBUNIT":1,"INTERACTION":3,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"PTM":1,"MISCELLANEOUS":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":6,"Compositional bias":4,"Cross-link":1,"Mutagenesis":2,"Sequence conflict":15},"uniParcId":"UPI0000251DC1"}}