{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A8FMC8","uniProtkbId":"FLIW_CAMJ8","entryAudit":{"firstPublicDate":"2008-02-26","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-11-13","entryVersion":82,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Campylobacter jejuni subsp. jejuni serotype O:6 (strain 81116 / NCTC 11828)","taxonId":407148,"lineage":["Bacteria","Pseudomonadati","Campylobacterota","Epsilonproteobacteria","Campylobacterales","Campylobacteraceae","Campylobacter"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01185"}],"value":"Flagellar assembly factor FliW"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01185"}],"value":"fliW"},"orderedLocusNames":[{"value":"C8J_1016"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01185"}],"value":"Acts as an anti-CsrA protein, binds CsrA and prevents it from repressing translation of its target genes, one of which is flagellin. Binds to flagellin and participates in the assembly of the flagellum"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27353476"}],"value":"Overexpression leads to increased levels of FlaA and FlaB, but levels of FlaC remain stable (PubMed:27353476). Involved in post-transcriptional regulation of flagellin biosynthesis (PubMed:27353476)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P96503"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27353476"}],"value":"Interacts with flagellins FlaA and FlaB but not with FlaC; recognizes glycosylated and non-glycosylated FlaA equally (PubMed:27353476, PubMed:28659885). Interacts with CsrA (PubMed:27353476). May form a 3-way complex of flagellin, FliS and FliW simultaneously in which FliS and FliW do not directly interact (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01185"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27353476"}],"value":"Cells grow faster and are about 55% less motile in a swarming assay, flagellar filaments are severely shortened (PubMed:27353476). Decreased levels of flagellins FlaA and FlaB but not FlaC accumulate; 3-fold more flaA and 4-fold less flaB mRNA accumulate in mid-log phase (PubMed:27353476). In double csrA-fliW deletions, flagellin levels are slightly lower than wild-type (PubMed:27353476)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01185"}],"value":"Belongs to the FliW family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"Flagellar assembly factor FliW","featureId":"PRO_1000073083"}],"keywords":[{"id":"KW-1005","category":"Biological process","name":"Bacterial flagellum biogenesis"},{"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0810","category":"Biological process","name":"Translation regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"17873037","citationType":"journal article","authors":["Pearson B.M.","Gaskin D.J.H.","Segers R.P.A.M.","Wells J.M.","Nuijten P.J.M.","van Vliet A.H.M."],"citationCrossReferences":[{"database":"PubMed","id":"17873037"},{"database":"DOI","id":"10.1128/jb.01404-07"}],"title":"The complete genome sequence of Campylobacter jejuni strain 81116 (NCTC11828).","publicationDate":"2007","journal":"J. Bacteriol.","firstPage":"8402","lastPage":"8403","volume":"189"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"81116 / NCTC 11828","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27353476","citationType":"journal article","authors":["Radomska K.A.","Ordonez S.R.","Woesten M.M.","Wagenaar J.A.","van Putten J.P."],"citationCrossReferences":[{"database":"PubMed","id":"27353476"},{"database":"DOI","id":"10.1111/mmi.13455"}],"title":"Feedback control of Campylobacter jejuni flagellin levels through reciprocal binding of FliW to flagellin and the global regulator CsrA.","publicationDate":"2016","journal":"Mol. Microbiol.","firstPage":"207","lastPage":"220","volume":"102"},"referencePositions":["FUNCTION","INTERACTION WITH FLAGELLIN","INTERACTION WITH CSRA","SUBUNIT","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"81116 / NCTC 11828","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"28659885","citationType":"journal article","authors":["Radomska K.A.","Woesten M.M.S.M.","Ordonez S.R.","Wagenaar J.A.","van Putten J.P.M."],"citationCrossReferences":[{"database":"PubMed","id":"28659885"},{"database":"DOI","id":"10.3389/fmicb.2017.01060"}],"title":"Importance of Campylobacter jejuni FliS and FliW in flagella biogenesis and flagellin secretion.","publicationDate":"2017","journal":"Front. Microbiol.","firstPage":"1060","lastPage":"1060","volume":"8"},"referencePositions":["INTERACTION WITH FLAA","SUBUNIT"],"referenceComments":[{"value":"81116 / NCTC 11828","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000814","properties":[{"key":"ProteinId","value":"ABV52615.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_002852982.1","properties":[{"key":"NucleotideSequenceId","value":"NC_009839.1"}]},{"database":"AlphaFoldDB","id":"A8FMC8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A8FMC8","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cju:C8J_1016","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_112356_2_0_7","properties":[{"key":"Description","value":"-"}]},{"database":"PHI-base","id":"PHI:8389","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0044780","properties":[{"key":"GoTerm","value":"P:bacterial-type flagellum assembly"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"2.30.290.10","properties":[{"key":"EntryName","value":"BH3618-like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01185","properties":[{"key":"EntryName","value":"FliW"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003775","properties":[{"key":"EntryName","value":"Flagellar_assembly_factor_FliW"}]},{"database":"InterPro","id":"IPR024046","properties":[{"key":"EntryName","value":"Flagellar_assmbl_FliW_dom_sf"}]},{"database":"NCBIfam","id":"NF009790","properties":[{"key":"EntryName","value":"PRK13282.1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39190","properties":[{"key":"EntryName","value":"FLAGELLAR ASSEMBLY FACTOR FLIW"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39190:SF1","properties":[{"key":"EntryName","value":"FLAGELLAR ASSEMBLY FACTOR FLIW"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02623","properties":[{"key":"EntryName","value":"FliW"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF141457","properties":[{"key":"EntryName","value":"BH3618-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTLAVKCPILGFEETKNMEFSTIDEVFVRLKSLDGKDFSFVLINPYLIRPDYEFDIPTYYQELLSLTPESNMKIFNIVAIAKSIEESTVNFLAPVVINLDNNTMVQVILDTVNYPDFFQADQIANYIKK","length":129,"molWeight":14844,"crc64":"B16779B934EDEF31","md5":"8075A3E3C9F18F4E5E4E52C134940C5F"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00000C1E01"}}