{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A7LXT0","uniProtkbId":"GH31A_BACO1","entryAudit":{"firstPublicDate":"2014-03-19","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-10-02","entryVersion":70,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153)","taxonId":411476,"lineage":["Bacteria","Pseudomonadati","Bacteroidota","Bacteroidia","Bacteroidales","Bacteroidaceae","Bacteroides"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Alpha-xylosidase BoGH31A"},"ecNumbers":[{"value":"3.2.1.177"}]},"alternativeNames":[{"fullName":{"value":"Glycosyl hydrolase family protein 31A"},"shortNames":[{"value":"BoGH31A"}]}],"flag":"Precursor"},"genes":[{"orfNames":[{"value":"BACOVA_02646"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}],"value":"Catalyzes the liberation of alpha-xylose from the non-reducing terminal glucose of xyloglucan oligosaccharides in xyloglucan degradation, converting the 'X' to 'G' units"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.","ecNumber":"3.2.1.177","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":7.7,"unit":"mM","substrate":"Xyl-alpha-PNP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}]},{"constant":31.8,"unit":"mM","substrate":"Glc-alpha-PNP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}]},{"constant":0.223,"unit":"mM","substrate":"XXXG","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}]},{"constant":0.378,"unit":"mM","substrate":"XLLG","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}]},{"constant":38.1,"unit":"mM","substrate":"isoprimeverose","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}]}],"note":{"texts":[{"value":"kcat is 1.6 sec(-1) for Xyl-alpha-PNP. kcat is 0.071 sec(-1) for Glc-alpha-PNP. kcat is 32.6 sec(-1) for XXXG. kcat is 31.0 sec(-1) for XLLG. kcat is 1.78 sec(-1) for isoprimeverose."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}],"value":"Optimum pH is 6.0-7.0."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}],"value":"Glucan metabolism; xyloglucan degradation"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}],"value":"Cell inner membrane localization is predicted by analogy with the archetypal sus locus"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}],"value":"Lipid-anchor","id":"SL-9901"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24463512"}],"value":"Severely reduced growth on tamarind xyloglucan and completely abolished growth on XyG oligosaccharides"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24463512"}],"value":"Gut bacteria supply the human body with energy from dietary polysaccharides through glycosidases that are absent in the human genome. Xyloglucans are a ubiquitous family of highly branched plant cell wall polysaccharides present in the vegetables we consume. Enzymes involved in xyloglucan degradation mediate the conversion of otherwise indigestible plant polysaccharides to short-chain fatty acids (PubMed:24463512)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyl hydrolase 31 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Chain","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":954,"modifier":"EXACT"}},"description":"Alpha-xylosidase BoGH31A","featureId":"PRO_0000425896"},{"type":"Domain","location":{"start":{"value":227,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"PA14","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01164"}]},{"type":"Active site","location":{"start":{"value":553,"modifier":"EXACT"},"end":{"value":553,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":556,"modifier":"EXACT"},"end":{"value":556,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":630,"modifier":"EXACT"},"end":{"value":630,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Lipidation","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"N-palmitoyl cysteine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Lipidation","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"S-diacylglycerol cysteine","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00303"}]},{"type":"Beta strand","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":28,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":57,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":93,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":107,"modifier":"EXACT"},"end":{"value":109,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":138,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":158,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":192,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":193,"modifier":"EXACT"},"end":{"value":195,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":196,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":221,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":238,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":240,"modifier":"EXACT"},"end":{"value":242,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":245,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":259,"modifier":"EXACT"},"end":{"value":262,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":284,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":294,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":302,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":305,"modifier":"EXACT"},"end":{"value":313,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":319,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":340,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":348,"modifier":"EXACT"},"end":{"value":353,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":358,"modifier":"EXACT"},"end":{"value":361,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":363,"modifier":"EXACT"},"end":{"value":380,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":384,"modifier":"EXACT"},"end":{"value":395,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":397,"modifier":"EXACT"},"end":{"value":402,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":403,"modifier":"EXACT"},"end":{"value":406,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":407,"modifier":"EXACT"},"end":{"value":410,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":418,"modifier":"EXACT"},"end":{"value":431,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":437,"modifier":"EXACT"},"end":{"value":440,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":447,"modifier":"EXACT"},"end":{"value":449,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOV"}]},{"type":"Turn","location":{"start":{"value":457,"modifier":"EXACT"},"end":{"value":459,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":463,"modifier":"EXACT"},"end":{"value":472,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":476,"modifier":"EXACT"},"end":{"value":481,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":483,"modifier":"EXACT"},"end":{"value":486,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":490,"modifier":"EXACT"},"end":{"value":497,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":504,"modifier":"EXACT"},"end":{"value":507,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":519,"modifier":"EXACT"},"end":{"value":523,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":528,"modifier":"EXACT"},"end":{"value":541,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":543,"modifier":"EXACT"},"end":{"value":545,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":549,"modifier":"EXACT"},"end":{"value":552,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":555,"modifier":"EXACT"},"end":{"value":557,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":565,"modifier":"EXACT"},"end":{"value":571,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":580,"modifier":"EXACT"},"end":{"value":582,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":602,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":610,"modifier":"EXACT"},"end":{"value":614,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":619,"modifier":"EXACT"},"end":{"value":622,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":623,"modifier":"EXACT"},"end":{"value":627,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":632,"modifier":"EXACT"},"end":{"value":634,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOV"}]},{"type":"Helix","location":{"start":{"value":635,"modifier":"EXACT"},"end":{"value":650,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":667,"modifier":"EXACT"},"end":{"value":679,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":684,"modifier":"EXACT"},"end":{"value":700,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":703,"modifier":"EXACT"},"end":{"value":708,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":711,"modifier":"EXACT"},"end":{"value":713,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":717,"modifier":"EXACT"},"end":{"value":720,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":726,"modifier":"EXACT"},"end":{"value":740,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":742,"modifier":"EXACT"},"end":{"value":754,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":759,"modifier":"EXACT"},"end":{"value":761,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":763,"modifier":"EXACT"},"end":{"value":766,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":771,"modifier":"EXACT"},"end":{"value":773,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":780,"modifier":"EXACT"},"end":{"value":782,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":783,"modifier":"EXACT"},"end":{"value":785,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":786,"modifier":"EXACT"},"end":{"value":788,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":797,"modifier":"EXACT"},"end":{"value":803,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":805,"modifier":"EXACT"},"end":{"value":807,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":809,"modifier":"EXACT"},"end":{"value":811,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":812,"modifier":"EXACT"},"end":{"value":814,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":820,"modifier":"EXACT"},"end":{"value":827,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":835,"modifier":"EXACT"},"end":{"value":838,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":842,"modifier":"EXACT"},"end":{"value":845,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":859,"modifier":"EXACT"},"end":{"value":865,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":870,"modifier":"EXACT"},"end":{"value":876,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":879,"modifier":"EXACT"},"end":{"value":882,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":883,"modifier":"EXACT"},"end":{"value":886,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":890,"modifier":"EXACT"},"end":{"value":897,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Turn","location":{"start":{"value":898,"modifier":"EXACT"},"end":{"value":901,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":902,"modifier":"EXACT"},"end":{"value":905,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Helix","location":{"start":{"value":906,"modifier":"EXACT"},"end":{"value":908,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":917,"modifier":"EXACT"},"end":{"value":927,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":941,"modifier":"EXACT"},"end":{"value":948,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]},{"type":"Beta strand","location":{"start":{"value":950,"modifier":"EXACT"},"end":{"value":953,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"5JOU"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0326","category":"Molecular function","name":"Glycosidase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0449","category":"PTM","name":"Lipoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0564","category":"PTM","name":"Palmitate"},{"id":"KW-0624","category":"Biological process","name":"Polysaccharide degradation"},{"id":"KW-0732","category":"Domain","name":"Signal"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-FV01NKS7U3NEA","citationType":"submission","authors":["Sudarsanam P.","Ley R.","Guruge J.","Turnbaugh P.J.","Mahowald M.","Liep D.","Gordon J."],"title":"Draft genome sequence of Bacteroides ovatus (ATCC 8483).","publicationDate":"APR-2007","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24463512","citationType":"journal article","authors":["Larsbrink J.","Rogers T.E.","Hemsworth G.R.","McKee L.S.","Tauzin A.S.","Spadiut O.","Klinter S.","Pudlo N.A.","Urs K.","Koropatkin N.M.","Creagh A.L.","Haynes C.A.","Kelly A.G.","Cederholm S.N.","Davies G.J.","Martens E.C.","Brumer H."],"citationCrossReferences":[{"database":"PubMed","id":"24463512"},{"database":"DOI","id":"10.1038/nature12907"}],"title":"A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes.","publicationDate":"2014","journal":"Nature","firstPage":"498","lastPage":"502","volume":"506"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","PATHWAY","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AAXF02000049","properties":[{"key":"ProteinId","value":"EDO11437.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_004298432.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_DS264579.1"}]},{"database":"PDB","id":"5JOU","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.50 A"},{"key":"Chains","value":"A=22-954"}]},{"database":"PDB","id":"5JOV","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.50 A"},{"key":"Chains","value":"A=22-954"}]},{"database":"PDBsum","id":"5JOU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5JOV","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"A7LXT0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A7LXT0","properties":[{"key":"Description","value":"-"}]},{"database":"CAZy","id":"GH31","properties":[{"key":"FamilyName","value":"Glycoside Hydrolase Family 31"}]},{"database":"GeneID","id":"29452173","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1501","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_000631_7_3_10","properties":[{"key":"Description","value":"-"}]},{"database":"SABIO-RK","id":"A7LXT0","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA01045","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0061634","properties":[{"key":"GoTerm","value":"F:alpha-D-xyloside xylohydrolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0030246","properties":[{"key":"GoTerm","value":"F:carbohydrate binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004553","properties":[{"key":"GoTerm","value":"F:hydrolase activity, hydrolyzing O-glycosyl compounds"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"24463512"}]},{"database":"GO","id":"GO:0085030","properties":[{"key":"GoTerm","value":"P:symbiotic process benefiting host"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"24463512"}]},{"database":"GO","id":"GO:2000899","properties":[{"key":"GoTerm","value":"P:xyloglucan catabolic process"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"24463512"}]},{"database":"CDD","id":"cd14752","properties":[{"key":"EntryName","value":"GH31_N"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd06591","properties":[{"key":"EntryName","value":"GH31_xylosidase_XylS"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.20.20.80:FF:000346","properties":[{"key":"EntryName","value":"Alpha-xylosidase BoGH31A"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.60.40.1180:FF:000023","properties":[{"key":"EntryName","value":"neutral alpha-glucosidase AB isoform X2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.120.380","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.20.20.80","properties":[{"key":"EntryName","value":"Glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.1760","properties":[{"key":"EntryName","value":"glycosyl hydrolase (family 31)"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.1180","properties":[{"key":"EntryName","value":"Golgi alpha-mannosidase II"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR033403","properties":[{"key":"EntryName","value":"DUF5110"}]},{"database":"InterPro","id":"IPR011013","properties":[{"key":"EntryName","value":"Gal_mutarotase_sf_dom"}]},{"database":"InterPro","id":"IPR017853","properties":[{"key":"EntryName","value":"GH_hydrolase_sf"}]},{"database":"InterPro","id":"IPR048395","properties":[{"key":"EntryName","value":"Glyco_hydro_31_C"}]},{"database":"InterPro","id":"IPR025887","properties":[{"key":"EntryName","value":"Glyco_hydro_31_N_dom"}]},{"database":"InterPro","id":"IPR000322","properties":[{"key":"EntryName","value":"Glyco_hydro_31_TIM"}]},{"database":"InterPro","id":"IPR013780","properties":[{"key":"EntryName","value":"Glyco_hydro_b"}]},{"database":"InterPro","id":"IPR051816","properties":[{"key":"EntryName","value":"Glycosyl_Hydrolase_31"}]},{"database":"InterPro","id":"IPR037524","properties":[{"key":"EntryName","value":"PA14/GLEYA"}]},{"database":"PANTHER","id":"PTHR43863","properties":[{"key":"EntryName","value":"HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G03140)-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43863:SF2","properties":[{"key":"EntryName","value":"MALTASE-GLUCOAMYLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17137","properties":[{"key":"EntryName","value":"DUF5110"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13802","properties":[{"key":"EntryName","value":"Gal_mutarotas_2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01055","properties":[{"key":"EntryName","value":"Glyco_hydro_31_2nd"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21365","properties":[{"key":"EntryName","value":"Glyco_hydro_31_3rd"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51445","properties":[{"key":"EntryName","value":"(Trans)glycosidases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56988","properties":[{"key":"EntryName","value":"Anthrax protective antigen"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF74650","properties":[{"key":"EntryName","value":"Galactose mutarotase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51011","properties":[{"key":"EntryName","value":"Glycosyl hydrolase domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51820","properties":[{"key":"EntryName","value":"PA14"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51257","properties":[{"key":"EntryName","value":"PROKAR_LIPOPROTEIN"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIMNMKNIFYCLLPGLLLGACSNKVYEKTGDSVIVKVQHKETGGPRLVRLQVMGDKLIHVSATADSKFADPQSLIVVPQKKQTSFAVVQNGDTITVSTEEVKASVLASTGEVWFTDKNGELILQENKGGGKTFTPIEVEGTKGYTVCQVFESPEDEAFYGLGQHQADEFNYKGKNEELFQYNTKVSVPFVVSNKNYGILLDSYSFCRFGNPNDYSQLNRIFKLYDKTGQEGALTGTYVPKKGETLVRREDSIYFENLKTIENLPKKLPLMGAKVTYEGEIEPAQTGEFKFILYYAGYVKVYLNNEPVVPERWRTAWNPNSYKFAAHLEAGKRVPLKIEWQPDGGQSYCGLRALTPVNPEEQGKQSWWSEMTKQLDYYFMAGENMDDVISGYRSLTGKSPVMPKWAMGFWQSREKYNTQEEMLGALKGFRDRKIPLDNIVLDWNHWPENAWGSHEFDKARFPDPKAMVDSIHAMHARMMISVWPKFYVTTEHFKEFDENGWMYQQSVKDSLKDWVGPGYHYGFYDAYDPDARKLFWKQMYEHYYPLGIDAWWMDASEPNVRDCTDLEYRKALCGPTALGSSTEFFNAYALMNAEAIYDGQRGVDNNKRVFLLTRSGFAGLQRYSTATWSGDIGTRWEDMKAQISAGLNFAMSGIPYWTMDIGGFCVENRYVAGQKQWNATKTENADYKEWRELNTRWYQFGAFVPLYRAHGQYPFREIWEIAPEGHPAYQSVVYYTKLRYNMMPYIYSLAGMTWFDDYTIMRPLVMDFTADAEVNDIGDQFMFGPSFMVSPVYRYGDRSREIYFPQAEGWYDFYSGKFQAGGERKVIEAPYERIPLYVRAGAIIPFGDDIQYTDEKPAEHIRLYIYQGADGEFTLYEDEGVNYNYEQGMYAMIPMKYDEATKTLVIGERQGEFPGMLKERTFTVVTVNKEKAQPFDLNAKGVTVKYNGSEQTLKL","length":954,"molWeight":109785,"crc64":"DA1FFF7BC54AFF75","md5":"E95BDAFA0513878F0524C4D9F2AA81A8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Signal":1,"Chain":1,"Domain":1,"Active site":3,"Lipidation":2,"Beta strand":57,"Helix":28,"Turn":8},"uniParcId":"UPI00015AB304"}}