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Involved in the cytoplasmic preassembly of dyneins into complexes before their transport into cilia (PubMed:34553759). Essential for the development of axonemal dynein motors in the sensory cilium of mechanosensory chordotonal (Ch) neurons and sperm flagellum, and consequently, is required for the mechanotransduction process of hearing and sperm mobility (PubMed:34553759)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34553759"}],"value":"Detected in the cytoplasm of differentiating motile ciliated cells including the chordotonal (Ch) neurons, spermatocytes and spermatids"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34553759"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q32NQ7"}],"value":"Dynein axonemal particle","id":"SL-0542"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34553759"}],"value":"Expressed in mechanosensory chordotonal (Ch) neurons, spermatocytes and spermatids (at protein level)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34553759"}],"value":"In stage 16 embryo, restricted to all differentiating mechanosensory chordotonal (Ch) neurons (lch5, v'ch1, and vchAB) (at protein level) (PubMed:34553759). In pupae, expression is restricted to the neuronal cell bodies and dendrite inner segments of Ch neurons within the Johnston's organ (at protein level) (PubMed:34553759)"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34553759"}],"value":"Displays impaired motility of cilia/flagella (PubMed:34553759). Adult males are infertile due to immotile sperm with flagella that lack dynein arms and display axoneme disruption (PubMed:34553759). However, the testes appear normal in shape and contain elongating flagellar bundles of spermatids (PubMed:34553759). Larvae fail to react to tone stimulus due to defective auditory/vibration mechanotransduction (PubMed:34553759). RNAi-mediated knockdown in the sensory neurons of females results in uncoordinated locomotion during climbing (PubMed:34553759)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DNAAF3 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":540,"modifier":"EXACT"}},"description":"Dynein axonemal assembly factor 3 homolog","featureId":"PRO_0000297584"},{"type":"Region","location":{"start":{"value":480,"modifier":"EXACT"},"end":{"value":499,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":316,"modifier":"EXACT"},"end":{"value":316,"modifier":"EXACT"}},"description":"in Ref. 3; 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