{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A1ZA77","secondaryAccessions":["Q8SZE6"],"uniProtkbId":"TM208_DROME","entryAudit":{"firstPublicDate":"2024-10-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-02-06","entryVersion":131,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Drosophila melanogaster","commonName":"Fruit fly","taxonId":7227,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000803"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Arthropoda","Altocrustacea","Allotriocarida","Hexapoda","Insecta","Pterygota","Neoptera","Eumetabola","Endopterygota","Aparaglossata","Panorpida","Diptera","Brachycera","Muscomorpha","Ephydroidea","Drosophilidae","Drosophila","Sophophora"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0034059"}],"value":"Transmembrane protein 208"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"38381787"},{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0034059"}],"value":"Tmem208"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"FlyBase","id":"FBgn0034059"}],"value":"CG8320"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38381787"}],"value":"May play an important role during development and helps to maintain proper levels of Fz"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38381787"}],"value":"Interacts with fz"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38381787"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38381787"}],"value":"Expressed in the brain"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38381787"}],"value":"Expressed in larval, pupal, and adult stages. In the 3rd instar larvae, broadly expressed in almost all tissues including wing disk, leg disk, eye disk, brain, salivary gland, anterior gut, fat body and Malpighian tubules"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"38381787"}],"value":"Results in lethality and the few escapers show a significantly reduced life span as well as defective leg, eye and wing development. Exhibit PCP-like defects in the eyes and wings and display a modest but consistent ER stress response"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TMEM208 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Transmembrane protein 208","featureId":"PRO_0000461083"},{"type":"Transmembrane","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":54,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":163,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"in Ref. 3; AAL48551","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"10731132","citationType":"journal article","authors":["Adams M.D.","Celniker S.E.","Holt R.A.","Evans C.A.","Gocayne J.D.","Amanatides P.G.","Scherer S.E.","Li P.W.","Hoskins R.A.","Galle R.F.","George R.A.","Lewis S.E.","Richards S.","Ashburner M.","Henderson S.N.","Sutton G.G.","Wortman J.R.","Yandell M.D.","Zhang Q.","Chen L.X.","Brandon R.C.","Rogers Y.-H.C.","Blazej R.G.","Champe M.","Pfeiffer B.D.","Wan K.H.","Doyle C.","Baxter E.G.","Helt G.","Nelson C.R.","Miklos G.L.G.","Abril J.F.","Agbayani A.","An H.-J.","Andrews-Pfannkoch C.","Baldwin D.","Ballew R.M.","Basu A.","Baxendale J.","Bayraktaroglu L.","Beasley E.M.","Beeson K.Y.","Benos P.V.","Berman B.P.","Bhandari D.","Bolshakov S.","Borkova D.","Botchan M.R.","Bouck J.","Brokstein P.","Brottier P.","Burtis K.C.","Busam D.A.","Butler H.","Cadieu E.","Center A.","Chandra I.","Cherry J.M.","Cawley S.","Dahlke C.","Davenport L.B.","Davies P.","de Pablos B.","Delcher A.","Deng Z.","Mays A.D.","Dew I.","Dietz S.M.","Dodson K.","Doup L.E.","Downes M.","Dugan-Rocha S.","Dunkov B.C.","Dunn P.","Durbin K.J.","Evangelista C.C.","Ferraz C.","Ferriera S.","Fleischmann W.","Fosler C.","Gabrielian A.E.","Garg N.S.","Gelbart W.M.","Glasser K.","Glodek A.","Gong F.","Gorrell J.H.","Gu Z.","Guan P.","Harris M.","Harris N.L.","Harvey D.A.","Heiman T.J.","Hernandez J.R.","Houck J.","Hostin D.","Houston K.A.","Howland T.J.","Wei M.-H.","Ibegwam C.","Jalali M.","Kalush F.","Karpen G.H.","Ke Z.","Kennison J.A.","Ketchum K.A.","Kimmel B.E.","Kodira C.D.","Kraft C.L.","Kravitz S.","Kulp D.","Lai Z.","Lasko P.","Lei Y.","Levitsky A.A.","Li J.H.","Li Z.","Liang Y.","Lin X.","Liu X.","Mattei B.","McIntosh T.C.","McLeod M.P.","McPherson D.","Merkulov G.","Milshina N.V.","Mobarry C.","Morris J.","Moshrefi A.","Mount S.M.","Moy M.","Murphy B.","Murphy L.","Muzny D.M.","Nelson D.L.","Nelson D.R.","Nelson K.A.","Nixon K.","Nusskern D.R.","Pacleb J.M.","Palazzolo M.","Pittman G.S.","Pan S.","Pollard J.","Puri V.","Reese M.G.","Reinert K.","Remington K.","Saunders R.D.C.","Scheeler F.","Shen H.","Shue B.C.","Siden-Kiamos I.","Simpson M.","Skupski M.P.","Smith T.J.","Spier E.","Spradling A.C.","Stapleton M.","Strong R.","Sun E.","Svirskas R.","Tector C.","Turner R.","Venter E.","Wang A.H.","Wang X.","Wang Z.-Y.","Wassarman D.A.","Weinstock G.M.","Weissenbach J.","Williams S.M.","Woodage T.","Worley K.C.","Wu D.","Yang S.","Yao Q.A.","Ye J.","Yeh R.-F.","Zaveri J.S.","Zhan M.","Zhang G.","Zhao Q.","Zheng L.","Zheng X.H.","Zhong F.N.","Zhong W.","Zhou X.","Zhu S.C.","Zhu X.","Smith H.O.","Gibbs R.A.","Myers E.W.","Rubin G.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10731132"},{"database":"DOI","id":"10.1126/science.287.5461.2185"}],"title":"The genome sequence of Drosophila melanogaster.","publicationDate":"2000","journal":"Science","firstPage":"2185","lastPage":"2195","volume":"287"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"12537572","citationType":"journal article","authors":["Misra S.","Crosby M.A.","Mungall C.J.","Matthews B.B.","Campbell K.S.","Hradecky P.","Huang Y.","Kaminker J.S.","Millburn G.H.","Prochnik S.E.","Smith C.D.","Tupy J.L.","Whitfield E.J.","Bayraktaroglu L.","Berman B.P.","Bettencourt B.R.","Celniker S.E.","de Grey A.D.N.J.","Drysdale R.A.","Harris N.L.","Richter J.","Russo S.","Schroeder A.J.","Shu S.Q.","Stapleton M.","Yamada C.","Ashburner M.","Gelbart W.M.","Rubin G.M.","Lewis S.E."],"citationCrossReferences":[{"database":"PubMed","id":"12537572"},{"database":"DOI","id":"10.1186/gb-2002-3-12-research0083"}],"title":"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.","publicationDate":"2002","journal":"Genome Biol.","firstPage":"RESEARCH0083.1","lastPage":"RESEARCH0083.22","volume":"3"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-BQ4L1BJG5E05U","citationType":"submission","authors":["Stapleton M.","Brokstein P.","Hong L.","Agbayani A.","Carlson J.","Champe M.","Chavez C.","Dorsett V.","Dresnek D.","Farfan D.","Frise E.","George R.","Gonzalez M.","Guarin H.","Kronmiller B.","Li P.","Liao G.","Miranda A.","Mungall C.J.","Nunoo J.","Pacleb J.","Paragas V.","Park S.","Patel S.","Phouanenavong S.","Wan K.","Yu C.","Lewis S.E.","Rubin G.M.","Celniker S."],"publicationDate":"DEC-2001","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"CI-133IMH7MAHTTP","citationType":"submission","authors":["Carlson J.","Booth B.","Frise E.","Park S.","Wan K.","Yu C.","Celniker S."],"publicationDate":"SEP-2008","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Berkeley","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"38381787","citationType":"journal article","authoringGroup":["Undiagnosed Diseases Network"],"authors":["Dutta D.","Kanca O.","Shridharan R.V.","Marcogliese P.C.","Steger B.","Morimoto M.","Frost F.G.","Macnamara E.","Wangler M.F.","Yamamoto S.","Jenny A.","Adams D.","Malicdan M.C.","Bellen H.J."],"citationCrossReferences":[{"database":"PubMed","id":"38381787"},{"database":"DOI","id":"10.1073/pnas.2322582121"}],"title":"Loss of the endoplasmic reticulum protein Tmem208 affects cell polarity, development, and viability.","publicationDate":"2024","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"e2322582121","lastPage":"e2322582121","volume":"121"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","SUBCELLULAR LOCATION","INTERACTION WITH FZ","DEVELOPMENTAL STAGE","TISSUE SPECIFICITY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE013599","properties":[{"key":"ProteinId","value":"AAF58083.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY070929","properties":[{"key":"ProteinId","value":"AAL48551.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT044328","properties":[{"key":"ProteinId","value":"ACH92393.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_611072.2","properties":[{"key":"NucleotideSequenceId","value":"NM_137228.4"}]},{"database":"AlphaFoldDB","id":"A1ZA77","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"A1ZA77","properties":[{"key":"Number of interactors","value":"77"}]},{"database":"IntAct","id":"A1ZA77","properties":[{"key":"Interactions","value":"10"}]},{"database":"STRING","id":"7227.FBpp0086344","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"7227-FBpp0086344","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"A1ZA77","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"36759","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblMetazoa","id":"FBtr0087202","properties":[{"key":"ProteinId","value":"FBpp0086344"},{"key":"GeneId","value":"FBgn0034059"}]},{"database":"GeneID","id":"36759","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"dme:Dmel_CG8320","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"CG8320-RA","properties":[{"key":"OrganismName","value":"d. melanogaster"}]},{"database":"AGR","id":"FB:FBgn0034059","properties":[{"key":"Description","value":"-"}]},{"database":"FlyBase","id":"FBgn0034059","properties":[{"key":"GeneName","value":"Tmem208"}]},{"database":"VEuPathDB","id":"VectorBase:FBgn0034059","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3269","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_094308_3_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"A1ZA77","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"PIRAGWM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10012212at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"36759","properties":[{"key":"hits","value":"0 hits in 1 CRISPR screen"}]},{"database":"GenomeRNAi","id":"36759","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:A1ZA77","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000803","properties":[{"key":"Component","value":"Chromosome 2R"}]},{"database":"Bgee","id":"FBgn0034059","properties":[{"key":"ExpressionPatterns","value":"Expressed in spermatid in male reproductive gland and 131 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"A1ZA77","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IDA:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38381787"}]},{"database":"GO","id":"GO:0005773","properties":[{"key":"GoTerm","value":"C:vacuole"},{"key":"GoEvidenceType","value":"IEA:GOC"}]},{"database":"GO","id":"GO:0005102","properties":[{"key":"GoTerm","value":"F:signaling receptor binding"},{"key":"GoEvidenceType","value":"IPI:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000353","source":"PubMed","id":"38381787"}]},{"database":"GO","id":"GO:0090251","properties":[{"key":"GoTerm","value":"P:protein localization involved in establishment of planar polarity"},{"key":"GoEvidenceType","value":"IDA:FlyBase"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"38381787"}]},{"database":"GO","id":"GO:0006624","properties":[{"key":"GoTerm","value":"P:vacuolar protein processing"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"InterPro","id":"IPR008506","properties":[{"key":"EntryName","value":"SND2/TMEM208"}]},{"database":"PANTHER","id":"PTHR13505","properties":[{"key":"EntryName","value":"TRANSMEMBRANE PROTEIN 208"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13505:SF7","properties":[{"key":"EntryName","value":"TRANSMEMBRANE PROTEIN 208"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05620","properties":[{"key":"EntryName","value":"TMEM208_SND2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAPQQKGKQGTKGAKQIVEENKTTLTFYRNMAIGCAAPALLLSFLVFEVTKTSVFMHILSLLILGSSYQFMAFMSQAKYSESGALLDSGNDLNMEGGIAENVKDLIILTSGTLLLALISNYFWLVLLLAPIRAGWMLWGSVIQPWLSQRNAQDDNPQVDEKKQKKMDRRMRRMR","length":174,"molWeight":19564,"crc64":"E07D3EBFBEF8EE0D","md5":"326516F4873547409C2A813559156E53"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":3,"Region":1,"Compositional bias":1,"Sequence conflict":1},"uniParcId":"UPI0000082E4B"}}