{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A1D699","uniProtkbId":"SPB4_ASPF1","entryAudit":{"firstPublicDate":"2007-04-03","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":99,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Aspergillus fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)","commonName":"Neosartorya fischeri","taxonId":331117,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Pezizomycotina","Eurotiomycetes","Eurotiomycetidae","Eurotiales","Aspergillaceae","Aspergillus","Fumigati"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"ATP-dependent rRNA helicase spb4"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P25808"}],"value":"3.6.4.13"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P25808"}],"value":"spb4"},"orfNames":[{"value":"NFIA_064070"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P25808"}],"value":"ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits. Binds 90S pre-ribosomal particles and dissociates from pre-60S ribosomal particles after processing of 27SB pre-rRNA. Required for the normal formation of 18S rRNA through the processing of pre-rRNAs at sites A0, A1 and A2, and the normal formation of 25S and 5.8S rRNAs through the processing of pre-rRNAs at sites C1 and C2"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"ATP + H2O = ADP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:13065"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"3.6.4.13","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P25808"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P25808"}],"value":"Component of pre-60S ribosomal complexes"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P25808"}],"value":"Nucleus, nucleolus","id":"SL-0188"}}]},{"texts":[{"value":"The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":640,"modifier":"EXACT"}},"description":"ATP-dependent rRNA helicase spb4","featureId":"PRO_0000282706"},{"type":"Domain","location":{"start":{"value":45,"modifier":"EXACT"},"end":{"value":249,"modifier":"EXACT"}},"description":"Helicase ATP-binding","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00541"}]},{"type":"Domain","location":{"start":{"value":283,"modifier":"EXACT"},"end":{"value":437,"modifier":"EXACT"}},"description":"Helicase C-terminal","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00542"}]},{"type":"Region","location":{"start":{"value":531,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":607,"modifier":"EXACT"},"end":{"value":640,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":521,"modifier":"EXACT"},"end":{"value":629,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Motif","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"Q motif","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Motif","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"DEAD box","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Compositional bias","location":{"start":{"value":568,"modifier":"EXACT"},"end":{"value":582,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":583,"modifier":"EXACT"},"end":{"value":595,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":630,"modifier":"EXACT"},"end":{"value":640,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00541"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0347","category":"Molecular function","name":"Helicase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0690","category":"Biological process","name":"Ribosome biogenesis"},{"id":"KW-0694","category":"Molecular function","name":"RNA-binding"},{"id":"KW-0698","category":"Biological process","name":"rRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"18404212","citationType":"journal article","authors":["Fedorova N.D.","Khaldi N.","Joardar V.S.","Maiti R.","Amedeo P.","Anderson M.J.","Crabtree J.","Silva J.C.","Badger J.H.","Albarraq A.","Angiuoli S.","Bussey H.","Bowyer P.","Cotty P.J.","Dyer P.S.","Egan A.","Galens K.","Fraser-Liggett C.M.","Haas B.J.","Inman J.M.","Kent R.","Lemieux S.","Malavazi I.","Orvis J.","Roemer T.","Ronning C.M.","Sundaram J.P.","Sutton G.","Turner G.","Venter J.C.","White O.R.","Whitty B.R.","Youngman P.","Wolfe K.H.","Goldman G.H.","Wortman J.R.","Jiang B.","Denning D.W.","Nierman W.C."],"citationCrossReferences":[{"database":"PubMed","id":"18404212"},{"database":"DOI","id":"10.1371/journal.pgen.1000046"}],"title":"Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus.","publicationDate":"2008","journal":"PLoS Genet.","firstPage":"E1000046","lastPage":"E1000046","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DS027690","properties":[{"key":"ProteinId","value":"EAW21243.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"XP_001263140.1","properties":[{"key":"NucleotideSequenceId","value":"XM_001263139.1"}]},{"database":"AlphaFoldDB","id":"A1D699","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A1D699","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"331117.A1D699","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"NFIA_064070_t1","properties":[{"key":"ProteinId","value":"EAW21243.1"},{"key":"GeneId","value":"NFIA_064070"}]},{"database":"GeneID","id":"4589661","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"nfi:NFIA_064070","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"FungiDB:NFIA_064070","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0345","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_003041_26_4_1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AYKEHEC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"7396459at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006702","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0030686","properties":[{"key":"GoTerm","value":"C:90S preribosome"},{"key":"GoEvidenceType","value":"IEA:EnsemblFungi"}]},{"database":"GO","id":"GO:0005730","properties":[{"key":"GoTerm","value":"C:nucleolus"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"IEA:EnsemblFungi"}]},{"database":"GO","id":"GO:0030687","properties":[{"key":"GoTerm","value":"C:preribosome, large subunit precursor"},{"key":"GoEvidenceType","value":"IEA:EnsemblFungi"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0016887","properties":[{"key":"GoTerm","value":"F:ATP hydrolysis activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0003676","properties":[{"key":"GoTerm","value":"F:nucleic acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003724","properties":[{"key":"GoTerm","value":"F:RNA helicase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:1902626","properties":[{"key":"GoTerm","value":"P:assembly of large subunit precursor of preribosome"},{"key":"GoEvidenceType","value":"IEA:EnsemblFungi"}]},{"database":"GO","id":"GO:0000470","properties":[{"key":"GoTerm","value":"P:maturation of LSU-rRNA"},{"key":"GoEvidenceType","value":"IEA:EnsemblFungi"}]},{"database":"CDD","id":"cd17960","properties":[{"key":"EntryName","value":"DEADc_DDX55"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd18787","properties":[{"key":"EntryName","value":"SF2_C_DEAD"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR056330","properties":[{"key":"EntryName","value":"CTT_SPB4"}]},{"database":"InterPro","id":"IPR011545","properties":[{"key":"EntryName","value":"DEAD/DEAH_box_helicase_dom"}]},{"database":"InterPro","id":"IPR014001","properties":[{"key":"EntryName","value":"Helicase_ATP-bd"}]},{"database":"InterPro","id":"IPR001650","properties":[{"key":"EntryName","value":"Helicase_C-like"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR000629","properties":[{"key":"EntryName","value":"RNA-helicase_DEAD-box_CS"}]},{"database":"InterPro","id":"IPR014014","properties":[{"key":"EntryName","value":"RNA_helicase_DEAD_Q_motif"}]},{"database":"InterPro","id":"IPR025313","properties":[{"key":"EntryName","value":"SPB4-like_CTE"}]},{"database":"PANTHER","id":"PTHR24031","properties":[{"key":"EntryName","value":"RNA HELICASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13959","properties":[{"key":"EntryName","value":"CTE_SPB4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF23681","properties":[{"key":"EntryName","value":"CTT_SPB4"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00270","properties":[{"key":"EntryName","value":"DEAD"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00271","properties":[{"key":"EntryName","value":"Helicase_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00487","properties":[{"key":"EntryName","value":"DEXDc"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01178","properties":[{"key":"EntryName","value":"DUF4217"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00490","properties":[{"key":"EntryName","value":"HELICc"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00039","properties":[{"key":"EntryName","value":"DEAD_ATP_HELICASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51192","properties":[{"key":"EntryName","value":"HELICASE_ATP_BIND_1"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51194","properties":[{"key":"EntryName","value":"HELICASE_CTER"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51195","properties":[{"key":"EntryName","value":"Q_MOTIF"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAPKPPSGTSSRAWDAVTPALSEWVLEAMSSMGFTRMTPVQASAIPLFMAHKDVVVEAVTGSGKTLSFLIPVVEKLLRLEEPIKKHHIGAIIISPTRELASQIYNVLSSLLAFHPPSAAAINPSEDDDAPRPKFPSSTLKVVPQLLLGGSTTPAEDLSTFLKRSPNVLVSTPGRLLELLSSPHVHCPQSSFEMLVLDEADRLLDLGFKETLQNILRRLPKQRRTGLFSASVSEAVDQIVRVGLRNPVKVMVKVKGGSGVDDKRTPASLQMTYLTTPPSHKFAALKRILSSVQPTPLKTIFFVSTCSGVDYLSAILPLLLGDDFLLIPLHGKHQANVRQKNFNRFINSHDPAILLTTDVAARGLDIPSVDLVVQIDPPSDPKSFIHRCGRAGRAGRRGLSVVLLHPGREEDYVSFLEVRKTPVVPFSPLITFSDADAAAATATARKAVLADRALHDRGQKAFVSWFRSYSKHQASSIFRVSDLDWEALGKAWGLLKLPKMPELRNFTGDKTLGVSLDWNNYAYKDKQREKRRKELLQEAAESGATQSTSNKRRATESVAWSQQAESKNKKLKRREQKKSKHEKARWEKMTEEEKQKVLETEKMVEELRKKNEEERRLRRAAAKAAGAKADGDDEEEFQGFD","length":640,"molWeight":71002,"crc64":"0560CEA14D5F0E65","md5":"1F4BE69F427DC3EF1A1A8E76CBAB12ED"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":2,"Region":2,"Coiled coil":1,"Motif":2,"Compositional bias":3,"Binding site":1},"uniParcId":"UPI0000EA70E4"}}