{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A1A5H6","secondaryAccessions":["Q6DRB0"],"uniProtkbId":"CNOT1_DANRE","entryAudit":{"firstPublicDate":"2008-01-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":103,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Danio rerio","commonName":"Zebrafish","synonyms":["Brachydanio rerio"],"taxonId":7955,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Actinopterygii","Neopterygii","Teleostei","Ostariophysi","Cypriniformes","Danionidae","Danioninae","Danio"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"CCR4-NOT transcription complex subunit 1"}},"alternativeNames":[{"fullName":{"value":"CCR4-associated factor 1"}}]},"genes":[{"geneName":{"value":"cnot1"},"orfNames":[{"value":"zgc:152902"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A5YKK6"}],"value":"Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3'UTRs. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A5YKK6"}],"value":"Component of the CCR4-NOT complex"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A5YKK6"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A5YKK6"}],"value":"Nucleus","id":"SL-0191"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A5YKK6"}],"value":"Contains Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, a motif known to be important for the association with nuclear receptors"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CNOT1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":2374,"modifier":"EXACT"}},"description":"CCR4-NOT transcription complex subunit 1","featureId":"PRO_0000315543"},{"type":"Region","location":{"start":{"value":1009,"modifier":"EXACT"},"end":{"value":1060,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1082,"modifier":"EXACT"},"end":{"value":1604,"modifier":"EXACT"}},"description":"Interaction with CCR4-NOT complex catalytic subunits","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Motif","location":{"start":{"value":153,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Motif","location":{"start":{"value":181,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Motif","location":{"start":{"value":223,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Motif","location":{"start":{"value":570,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Motif","location":{"start":{"value":1638,"modifier":"EXACT"},"end":{"value":1642,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Motif","location":{"start":{"value":1940,"modifier":"EXACT"},"end":{"value":1944,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Motif","location":{"start":{"value":2094,"modifier":"EXACT"},"end":{"value":2098,"modifier":"EXACT"}},"description":"LXXLL"},{"type":"Compositional bias","location":{"start":{"value":1009,"modifier":"EXACT"},"end":{"value":1051,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":424,"modifier":"EXACT"},"end":{"value":424,"modifier":"EXACT"}},"description":"in Ref. 2; AAT68167","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["Y"]}},{"type":"Sequence conflict","location":{"start":{"value":433,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"in Ref. 2; AAT68167","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["I"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-0943","category":"Biological process","name":"RNA-mediated gene silencing"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"id":"KW-0810","category":"Biological process","name":"Translation regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-6LS8RSIU9316C","citationType":"submission","authoringGroup":["NIH - Zebrafish Gene Collection (ZGC) project"],"publicationDate":"DEC-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"AB","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"15256591","citationType":"journal article","authors":["Amsterdam A.","Nissen R.M.","Sun Z.","Swindell E.C.","Farrington S.","Hopkins N."],"citationCrossReferences":[{"database":"PubMed","id":"15256591"},{"database":"DOI","id":"10.1073/pnas.0403929101"}],"title":"Identification of 315 genes essential for early zebrafish development.","publicationDate":"2004","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"12792","lastPage":"12797","volume":"101"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1595"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BC128655","properties":[{"key":"ProteinId","value":"AAI28656.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY648849","properties":[{"key":"ProteinId","value":"AAT68167.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001073420.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001079951.2"}]},{"database":"AlphaFoldDB","id":"A1A5H6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A1A5H6","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"A1A5H6","properties":[{"key":"Number of interactors","value":"2954"}]},{"database":"STRING","id":"7955.ENSDARP00000111504","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"A1A5H6","properties":[{"key":"glycosylation","value":"2 sites, 1 O-linked glycan (1 site)"}]},{"database":"PaxDb","id":"7955-ENSDARP00000111504","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"A1A5H6","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"448949","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"dre:448949","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"ZFIN:ZDB-GENE-040915-1","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"23019","properties":[{"key":"Description","value":"-"}]},{"database":"ZFIN","id":"ZDB-GENE-040915-1","properties":[{"key":"GeneName","value":"cnot1"}]},{"database":"eggNOG","id":"KOG1831","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"A1A5H6","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"1933107at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"A1A5H6","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-DRE-6804115","properties":[{"key":"PathwayName","value":"TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain"}]},{"database":"PRO","id":"PR:A1A5H6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000437","properties":[{"key":"Component","value":"Chromosome 25"}]},{"database":"GO","id":"GO:0030014","properties":[{"key":"GoTerm","value":"C:CCR4-NOT complex"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0030015","properties":[{"key":"GoTerm","value":"C:CCR4-NOT core complex"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0000932","properties":[{"key":"GoTerm","value":"C:P-body"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0060090","properties":[{"key":"GoTerm","value":"F:molecular adaptor activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0030331","properties":[{"key":"GoTerm","value":"F:nuclear estrogen receptor binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0042974","properties":[{"key":"GoTerm","value":"F:nuclear retinoic acid receptor binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0048589","properties":[{"key":"GoTerm","value":"P:developmental growth"},{"key":"GoEvidenceType","value":"IMP:ZFIN"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"25533962"}]},{"database":"GO","id":"GO:0033147","properties":[{"key":"GoTerm","value":"P:negative regulation of intracellular estrogen receptor signaling pathway"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0048387","properties":[{"key":"GoTerm","value":"P:negative regulation of retinoic acid receptor signaling pathway"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0000122","properties":[{"key":"GoTerm","value":"P:negative regulation of transcription by RNA polymerase II"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0017148","properties":[{"key":"GoTerm","value":"P:negative regulation of translation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0000288","properties":[{"key":"GoTerm","value":"P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0010606","properties":[{"key":"GoTerm","value":"P:positive regulation of cytoplasmic mRNA processing body assembly"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:1900153","properties":[{"key":"GoTerm","value":"P:positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0060213","properties":[{"key":"GoTerm","value":"P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"CDD","id":"cd20710","properties":[{"key":"EntryName","value":"NOT1_connector"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.25.40.800:FF:000001","properties":[{"key":"EntryName","value":"CCR4-NOT transcription complex subunit 1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.25.40.180:FF:000005","properties":[{"key":"EntryName","value":"Ccr4-not transcription complex subunit 1 isoform"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.25.40.790:FF:000001","properties":[{"key":"EntryName","value":"Ccr4-not transcription complex subunit 1 isoform"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.25.40.840:FF:000001","properties":[{"key":"EntryName","value":"Ccr4-not transcription complex subunit 1 isoform"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.180","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.790","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.800","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.25.40.840","properties":[{"key":"EntryName","value":"CCR4-NOT transcription complex subunit 1 TTP binding domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007196","properties":[{"key":"EntryName","value":"CCR4-Not_Not1_C"}]},{"database":"InterPro","id":"IPR055454","properties":[{"key":"EntryName","value":"CNOT1-like_NOT1_connector"}]},{"database":"InterPro","id":"IPR055104","properties":[{"key":"EntryName","value":"CNOT1_1st"}]},{"database":"InterPro","id":"IPR032191","properties":[{"key":"EntryName","value":"CNOT1_CAF1_bind"}]},{"database":"InterPro","id":"IPR024557","properties":[{"key":"EntryName","value":"CNOT1_dom_4"}]},{"database":"InterPro","id":"IPR032194","properties":[{"key":"EntryName","value":"CNOT1_HEAT"}]},{"database":"InterPro","id":"IPR032193","properties":[{"key":"EntryName","value":"CNOT1_TTP_bind"}]},{"database":"InterPro","id":"IPR038535","properties":[{"key":"EntryName","value":"CNOT1_TTP_bind_sf"}]},{"database":"InterPro","id":"IPR040398","properties":[{"key":"EntryName","value":"Not1"}]},{"database":"PANTHER","id":"PTHR13162","properties":[{"key":"EntryName","value":"CCR4-NOT TRANSCRIPTION COMPLEX"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13162:SF8","properties":[{"key":"EntryName","value":"CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22940","properties":[{"key":"EntryName","value":"CNOT1_1st"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16415","properties":[{"key":"EntryName","value":"CNOT1_CAF1_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16418","properties":[{"key":"EntryName","value":"CNOT1_HEAT"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF16417","properties":[{"key":"EntryName","value":"CNOT1_TTP_bind"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12842","properties":[{"key":"EntryName","value":"DUF3819"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04054","properties":[{"key":"EntryName","value":"Not1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF23590","properties":[{"key":"EntryName","value":"NOT1_connector"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNLDSLSLALSQISYLVDNLTKKNYRASQQEIQHIVNRHGPEADRHLLRCLFSHVDFSGDGKSSGKDFHQTQFLIQECVSLITKPNFISTLCYAIDNPLHYQKSLKPSPHLFTQLSKVLKLSKVQEVILGLALSNSSNADLRGFAAQFVKQKLPDLLRSYVDADLGGNQEGGFQDIAIEVLHLLLSHLLFGQKGSSGVGQEQIDAFLKTLCRDFPQERCPVVLAPLLYPDKRDILMDRILPDSGDLNKTMMESSLADFMQEVGYGFCASLEECRNIILQYGVREVTASQVARVLGMMARTHSGLSDGISLQTITNPVGGGGIWSDGKDKSDSSQAWNVEVLIDVVKEVNPNLNFKEVTYELDHPGFLIRDSKGLQIVVYGIQRGLGMEVFPVDLIYRPWKHAEGQLSFIQHSLLSPEVFCFADNPCHTVAIDTLKAPPEDDNREIATWKSLDLVESLLRLSEVGHYEQVKQLFSFPIKHCPDMLVLALLQISTSWHTLRHELISTLMPIFLGNHPNSAIILHYAWHGQGQSPSIRQLIMHSMAEWYMRGEQYDQAKLSRILDVAQDLKSLSMLLNGTPFAFVIDLAALASRREYLKLDKWLTDKIREHGEPFIQACVTFLKRRCPSIMGGLAPEKDQPKSAQLPPETLATMLACLQSCAGSVSQELSETILTMVANCSNVMNKARQPPPGVLPKGRAPSTSSLDAISPVQMDPLSAMGSLSLGVSSTSHTPSMQGFPSLQGSAFSNPQSPAKAFSNLPNPNPSTAFPGINPLSSQLQGPLSTSLSGIGSGLGMPTVSSDVFSARKMSTPGLNPPTFQQTDLSQVWPEANQHFSKEIDDEANSYFQRIYNHPPHPTMSVDEVLEMLQRFKDSNIKREREVFNCMLRNLFEEYRFFPQYPDKELHITACLFGGIIEKGLVTYMALGLALRYVLEALRKPFGSKMYYFGIAALDRFKNRLKDYPQYCQHLASIAHFLQFPHHLQEYIEYGQQSRDPPVKMQGSITTPGSLALAQAQAQSQPPKAPQPGQASTLVTTATTTTTAAKTTTITRPTAVGPKKDVPPSINTTNIDTLLVATDQTERIVEPPENVQEKIAFIFNNLSQSNMSQKVEELKETVKEEFMPWVSQYLVMKRVSIEPNFHSLYSNFLDTLKNPEFVKMVLNETYRNIKVLLTSDKAAANFSDRSLLKNLGHWLGMITLAKNKPILYTDLELKSLLLEAYVKGQQELLYVVPFVAKVLESSLRSVIFRPQNPWTMGIMNVLAELHQEHDLKLNLKFEIEVLCKNLSMDITDLKPGNLLRDKDKLKTLEEQLSAPKKETKPPEELLPIVTTDSVPFTAAPSTPATTTACTATGPPTPQFSYHDINVYALAGLAPHININVNIPLLQAHPQLKQCVRPAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESHMRVAAHHMMRNLTAGMAMITCREPLLMSIATNLKNSFAAALRAPTPQQREMMEEAAARIAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPMVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPSNDLSQPTGFLAQPMKQQAWPTDDVAHIYEKCISDLEQHLHAIPPALAMNPQTQAIRSLLEAVVMARNSRDGIAALGLLQKAVEGLLDATSGADPELLLSYRECHLLVLKALQDGRAYGPQWCNKQITRCLIECRDEYKYNVEAVELLIRNHLVNMQQYDLHLAQSMENGLNYMAVAFAMQLVKLLLVDERSVSHITEADLFHTIETLMRTSAHSRANAPEGLPQLMDVVRSNYEAMIDRHHGGPNFMMHSGISQASEYDDPPGLREKAEYLLREWVNLYHSAAAGRDSTKAFSAFVGQMHQQGILKTDDLITRFFRLCTEMCVEISYRAQAEQQHPTTSPAIIRAKCYHNLDAFVRLIALLVKHSGEATNTVTKINLLNKVLGIVVGVLIQDHDVRQTEFQQLPYHRIFIMLLLELNAPEHVLETINFQTLTAFCNTFHILRPTKAPGFVYAWLELISHRIFIARMLAHTPQQKGWPMYAQLLIDLFKYLAPFLRNVELNKPMQILYKGTLRVLLVLLHDFPEFLCDYHYGFCDVIPPNCIQLRNLILSAFPRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPSQFKKDLDSYLKTRSPVTFLSELRSNLQVSNEPGNRYNIQLINALVPYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANAEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWSHDFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS","length":2374,"molWeight":266604,"crc64":"CAE9127310F44516","md5":"60046B5DD4BBE292F3FB5038B5DC9FEC"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":2,"Motif":7,"Compositional bias":1,"Sequence conflict":2},"uniParcId":"UPI0000EA2787"}}