{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A1A4L4","uniProtkbId":"MINY4_BOVIN","entryAudit":{"firstPublicDate":"2008-02-26","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":74,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Bos taurus","commonName":"Bovine","taxonId":9913,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Laurasiatheria","Artiodactyla","Ruminantia","Pecora","Bovidae","Bovinae","Bos"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable ubiquitin carboxyl-terminal hydrolase MINDY-4"},"ecNumbers":[{"value":"3.4.19.12"}]},"alternativeNames":[{"fullName":{"value":"Probable deubiquitinating enzyme MINDY-4"}}]},"genes":[{"geneName":{"value":"MINDY4"},"synonyms":[{"value":"FAM188B"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8NBR6"}],"value":"Probable hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).","ecNumber":"3.4.19.12","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8NBR6"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the MINDY deubiquitinase family. FAM188 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":763,"modifier":"EXACT"}},"description":"Probable ubiquitin carboxyl-terminal hydrolase MINDY-4","featureId":"PRO_0000320589"},{"type":"Region","location":{"start":{"value":154,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":267,"modifier":"EXACT"},"end":{"value":278,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":346,"modifier":"EXACT"},"end":{"value":355,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":357,"modifier":"EXACT"},"end":{"value":368,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":463,"modifier":"EXACT"},"end":{"value":463,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]},{"type":"Active site","location":{"start":{"value":683,"modifier":"EXACT"},"end":{"value":683,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N5J2"}]},{"type":"Modified residue","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q3UQI9"}]},{"type":"Modified residue","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":220,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q4G0A6"}]},{"type":"Modified residue","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q4G0A6"}]},{"type":"Modified residue","location":{"start":{"value":296,"modifier":"EXACT"},"end":{"value":296,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q4G0A6"}]}],"keywords":[{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0788","category":"Molecular function","name":"Thiol protease"},{"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1L3IUPBBQNSSQ","citationType":"submission","authoringGroup":["NIH - Mammalian Gene Collection (MGC) project"],"publicationDate":"OCT-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Crossbred X Angus","type":"STRAIN"},{"value":"Liver","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BC126671","properties":[{"key":"ProteinId","value":"AAI26672.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001073788.2","properties":[{"key":"NucleotideSequenceId","value":"NM_001080319.3"}]},{"database":"AlphaFoldDB","id":"A1A4L4","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"A1A4L4","properties":[{"key":"Number of interactors","value":"202"}]},{"database":"STRING","id":"9913.ENSBTAP00000012360","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"A1A4L4","properties":[{"key":"glycosylation","value":"1 site"}]},{"database":"PaxDb","id":"9913-ENSBTAP00000012360","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"615509","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bta:615509","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"84182","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG2871","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"A1A4L4","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"10263628at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000009136","properties":[{"key":"Component","value":"Unplaced"}]},{"database":"GO","id":"GO:0004843","properties":[{"key":"GoTerm","value":"F:cysteine-type deubiquitinase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:1990380","properties":[{"key":"GoTerm","value":"F:K48-linked deubiquitinase activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0071108","properties":[{"key":"GoTerm","value":"P:protein K48-linked deubiquitination"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR025257","properties":[{"key":"EntryName","value":"MINDY-3/4_CD"}]},{"database":"InterPro","id":"IPR059022","properties":[{"key":"EntryName","value":"MINDY4_N"}]},{"database":"InterPro","id":"IPR039785","properties":[{"key":"EntryName","value":"MINY3/4"}]},{"database":"PANTHER","id":"PTHR12473","properties":[{"key":"EntryName","value":"UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12473:SF8","properties":[{"key":"EntryName","value":"UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF26038","properties":[{"key":"EntryName","value":"Dimer_MINDY4_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13898","properties":[{"key":"EntryName","value":"MINDY-3_4_CD"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01174","properties":[{"key":"EntryName","value":"DUF4205"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDTLFVEEVAASLIREFLSRKGLKKTYVTMDQERPRSDLSINSRNDLRKVLHLEFLYKENKAKENPLKTNLELITRYFLDHFGNIGNNVTQETRIPELSVPKKSNKLPLRSSETTLVNIYHLADEDETWRTSLSEISKARHDSLDGDVLGHFVSSKRSSHKSRPIKTVAGESPTVASAWEKTDKLPMSEPSLDTKRMGEKVRPKSGLIVRGMMAGPIASSPQDSLRKRSLRRSPALSSATQPHKEGSPQEPELSTHTSTCPTPLEGPASSTASTSRSPQGPLSELTWEKQRTSPGSPPHLPGKGLLPRGSGRWRDLSEDSPAVDSGSEAIRTPPKFSLSSGNVPKTQERPERAFERQGSQPASLRKNQLSVSNKLEGDLDVLQLEDVEDELVREEIILSPVSSVLKLQVVSKPIDLSVAKDIKTILFGSSFCCFSDEWKLQSFSFNDSVSLKYGIVQNKGGPCGVLAAVQGCVLQKLLFEGDSSADCARLQPSNARRTHCLALAIADIVWRAGGCERAVVTLASGTQHFSPTGKYKADGVLETLILHSLTCYEELVTFLQQSIHQFEAGPYGCVLLTLSAILSRSTELVRQDFDVPTSHLIGAHGYCTQELVNLLLTGKAVSNVFNDVVELDSGNGDVTLLKGISTRSDIGFLSLFEHYNVCQVGCFLKTPRFPIWVVCSESHFSVLFSQQLELLRDWRAERLFDLYYYDGLANQQEQIRLTVDTTQTVPEDRDNGLVPPLELCNRTKWKGASVNWNGSEPIL","length":763,"molWeight":84447,"crc64":"A48F30F6D98CC401","md5":"198E5375A30262CEFF165DBAA008878D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Compositional bias":5,"Active site":2,"Modified residue":4},"uniParcId":"UPI0000EA36C0"}}