{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A1A4I9","uniProtkbId":"UFL1_BOVIN","entryAudit":{"firstPublicDate":"2008-04-08","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":100,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Bos taurus","commonName":"Bovine","taxonId":9913,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Laurasiatheria","Artiodactyla","Ruminantia","Pecora","Bovidae","Bovinae","Bos"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"E3 UFM1-protein ligase 1"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"2.3.2.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31721015"}],"value":"E3 UFM1-protein transferase 1"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31721015"}],"value":"UFL1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"},{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8CCJ3"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30881595"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31078114"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31721015"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32050508"}],"value":"E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy) (By similarity). Catalyzes ufmylation of many protein, such as CD274/PD-L1, CDK5RAP3, CYB5R3, DDRGK1, EIF6, histone H4, MRE11, P4HB, PDCD1/PD-1, TRIP4, RPN1, RPS20/uS10, RPL10/uL16, RPL26/uL24, SYVN1/HRD1 and TP53/p53 (By similarity). As part of the UREL complex, plays a key role in ribosome recycling by catalyzing mono-ufmylation of RPL26/uL24 subunit of the 60S ribosome (By similarity). Ufmylation of RPL26/uL24 occurs on free 60S ribosomes following ribosome dissociation: it weakens the junction between post-termination 60S subunits and SEC61 translocons, promoting release and recycling of the large ribosomal subunit from the endoplasmic reticulum membrane (By similarity). Ufmylation of RPL26/uL24 and subsequent 60S ribosome recycling either take place after normal termination of translation or after ribosome stalling during cotranslational translocation at the endoplasmic reticulum (By similarity). Involved in reticulophagy in response to endoplasmic reticulum stress by mediating ufmylation of proteins such as CYB5R3 and RPN1, thereby promoting lysosomal degradation of ufmylated proteins (By similarity). Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis, blood vessel morphogenesis or inflammatory response (PubMed:30881595). Regulates inflammation in response to endoplasmic reticulum stress by promoting reticulophagy, leading to inhibit the activity of the NF-kappa-B transcription factor (PubMed:30881595, PubMed:31078114, PubMed:31721015, PubMed:32050508). Mediates ufmylation of DDRGK1 and CDK5RAP3; the role of these modifications is however unclear: as both DDRGK1 and CDK5RAP3 act as substrate adapters for ufmylation, it is uncertain whether ufmylation of these proteins is, a collateral effect or is required for ufmylation (By similarity). Acts as a negative regulator of T-cell activation by mediating ufmylation and stabilization of PDCD1/PD-1 (By similarity). Also involved in the response to DNA damage: recruited to double-strand break sites following DNA damage and mediates monoufmylation of histone H4 and ufmylation of MRE11 (By similarity). Mediates ufmylation of TP53/p53, promoting its stability (By similarity). Catalyzes ufmylation of TRIP4, thereby playing a role in nuclear receptor-mediated transcription (By similarity). Required for hematopoietic stem cell function and hematopoiesis (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"Catalytic component of the UFM1 ribosome E3 ligase (UREL) complex, composed of UFL1, DDRGK1 and CDK5RAP3. Interacts with E2-like enzyme UFC1. Interacts with RELA. Interacts with NBN; promoting recruitment to double-strand breaks following DNA damage. Interacts (when phosphorylated) with YWHAG/14-3-3-gamma; sequestering UFL1 and preventing its association with PDCD1/PD-1 substrate"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"Recruited to double-strand breaks by the MRE11-RAD50-NBN (MRN) complex following DNA damage"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30881595"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31721015"}],"value":"Cytoplasm, cytosol","id":"SL-0091"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30881595"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31721015"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"Chromosome","id":"SL-0468"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30881595"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31721015"}],"value":"Expressed in response to endoplasmic reticulum stress (PubMed:31721015). By lipopolysaccharide (LPS) (PubMed:30881595)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects both proteins against ubiquitination and degradation via the proteasome"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}],"value":"Phosphorylation at Thr-534 by AMPK promotes its interaction with YWHAG/14-3-3-gamma, thereby preventing UFL1 association with PDCD1/PD-1 substrate"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UFL1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":792,"modifier":"EXACT"}},"description":"E3 UFM1-protein ligase 1","featureId":"PRO_0000328122"},{"type":"Region","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"Required for E3 UFM1-protein ligase activity","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Region","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":200,"modifier":"EXACT"}},"description":"Mediates interaction with DDRGK1","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Region","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":250,"modifier":"EXACT"}},"description":"Involved in CDK5RAP3-binding","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Region","location":{"start":{"value":200,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"Mediates interaction with TRIP4","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Region","location":{"start":{"value":412,"modifier":"EXACT"},"end":{"value":471,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":488,"modifier":"EXACT"},"end":{"value":682,"modifier":"EXACT"}},"description":"Mediates interaction with CDK5RAP3","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"B2GV24"}]},{"type":"Compositional bias","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":471,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"N-acetylalanine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Modified residue","location":{"start":{"value":433,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Omega-N-methylarginine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8CCJ3"}]},{"type":"Modified residue","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":458,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Modified residue","location":{"start":{"value":534,"modifier":"EXACT"},"end":{"value":534,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"O94874"}]},{"type":"Modified residue","location":{"start":{"value":752,"modifier":"EXACT"},"end":{"value":752,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8CCJ3"}]}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0158","category":"Cellular component","name":"Chromosome"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0488","category":"PTM","name":"Methylation"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"},{"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-1L3IUPBBQNSSQ","citationType":"submission","authoringGroup":["NIH - 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