{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0R5Z0","uniProtkbId":"BPA_MYCS2","entryAudit":{"firstPublicDate":"2015-10-14","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-09","entryVersion":95,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)","commonName":"Mycobacterium smegmatis","taxonId":246196,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Mycobacteriales","Mycobacteriaceae","Mycolicibacterium"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"25469515"}],"value":"Bacterial proteasome activator"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"25469515"}],"value":"bpa"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ABK74607.1"}],"value":"MSMEG_6365"},{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AFP42623.1"}],"value":"MSMEI_6197"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WKX3"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25469515"}],"value":"Interacts with the core proteasome alpha-subunit (PrcA) through its C-terminal hydrophobic-tyrosine-X motif (HbYX motif) (PubMed:25469515). Interaction of Bpa with the proteasome stimulates proteasomal peptidase and casein degradation activity, which suggests Bpa could play a role in the removal of non-native or damaged proteins by influencing the conformation of the proteasome complex upon interaction (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WKX3"}],"value":"Forms a homooligomeric, either hexameric or heptameric, ring-like structure which stacks co-axially with the proteasomal alpha-rings"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Bpa family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Bacterial proteasome activator","featureId":"PRO_0000434132"},{"type":"Region","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":173,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"HbYX motif","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P9WKX3"}]},{"type":"Mutagenesis","location":{"start":{"value":172,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Loss of interaction with the proteasome subunit PrcA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25469515"}],"alternativeSequence":{}}],"keywords":[{"id":"KW-0647","category":"Cellular component","name":"Proteasome"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-ET8TSAB7T4FCA","citationType":"submission","authors":["Fleischmann R.D.","Dodson R.J.","Haft D.H.","Merkel J.S.","Nelson W.C.","Fraser C.M."],"publicationDate":"OCT-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"17295914","citationType":"journal article","authors":["Deshayes C.","Perrodou E.","Gallien S.","Euphrasie D.","Schaeffer C.","Van-Dorsselaer A.","Poch O.","Lecompte O.","Reyrat J.-M."],"citationCrossReferences":[{"database":"PubMed","id":"17295914"},{"database":"DOI","id":"10.1186/gb-2007-8-2-r20"}],"title":"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?","publicationDate":"2007","journal":"Genome Biol.","firstPage":"R20.1","lastPage":"R20.9","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"18955433","citationType":"journal article","authors":["Gallien S.","Perrodou E.","Carapito C.","Deshayes C.","Reyrat J.-M.","Van Dorsselaer A.","Poch O.","Schaeffer C.","Lecompte O."],"citationCrossReferences":[{"database":"PubMed","id":"18955433"},{"database":"DOI","id":"10.1101/gr.081901.108"}],"title":"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol.","publicationDate":"2009","journal":"Genome Res.","firstPage":"128","lastPage":"135","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"25469515","citationType":"journal article","authors":["Delley C.L.","Laederach J.","Ziemski M.","Bolten M.","Boehringer D.","Weber-Ban E."],"citationCrossReferences":[{"database":"PubMed","id":"25469515"},{"database":"DOI","id":"10.1371/journal.pone.0114348"}],"title":"Bacterial proteasome activator Bpa (Rv3780) is a novel ring-shaped interactor of the mycobacterial proteasome.","publicationDate":"2014","journal":"PLoS ONE","firstPage":"E114348","lastPage":"E114348","volume":"9"},"referencePositions":["FUNCTION","INTERACTION WITH THE PROTEASOME SUBUNIT ALPHA PRCA","MUTAGENESIS OF 172-GLY--LEU-175"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000480","properties":[{"key":"ProteinId","value":"ABK74607.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP001663","properties":[{"key":"ProteinId","value":"AFP42623.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_011731234.1","properties":[{"key":"NucleotideSequenceId","value":"NC_008596.1"}]},{"database":"AlphaFoldDB","id":"A0R5Z0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0R5Z0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"246196.MSMEG_6365","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"246196-MSMEG_6365","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93460978","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"msb:LJ00_31460","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"msg:MSMEI_6197","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"msm:MSMEG_6365","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|246196.19.peg.6193","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"ENOG502ZPJ4","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"OrthoDB","id":"5189298at2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000757","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0070628","properties":[{"key":"GoTerm","value":"F:proteasome binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"25469515"}]},{"database":"GO","id":"GO:0061136","properties":[{"key":"GoTerm","value":"P:regulation of proteasomal protein catabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR019695","properties":[{"key":"EntryName","value":"Proteasome_act"}]},{"database":"Pfam","id":"PF10759","properties":[{"key":"EntryName","value":"BPA"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTINPDDDNIEILTGAAGGADTEGEGEGEGKSLTDLVEQPAKVMRIGTMIKQLLEEVRAAPLDDASRNRLREIHQTSIRELEDGLAPELREELERLTLPFTDDNVPSDAELRIAQAQLVGWLEGLFHGIQTALFAQQMAARAQLEQMRQGALPPGIQVPGAQRGGATHPGTGQYL","length":175,"molWeight":18944,"crc64":"99C7963D6ED82B42","md5":"F2731EA38D5D905CA06887D422B5C7DC"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Motif":1,"Mutagenesis":1},"uniParcId":"UPI0000E86111"}}