{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0R4D0","secondaryAccessions":["I7G8U6"],"uniProtkbId":"Y5790_MYCS2","entryAudit":{"firstPublicDate":"2010-08-10","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2007-01-09","entryVersion":94,"sequenceVersion":1},"annotationScore":1.0,"organism":{"scientificName":"Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)","commonName":"Mycobacterium smegmatis","taxonId":246196,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Mycobacteriales","Mycobacteriaceae","Mycolicibacterium"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Uncharacterized protein MSMEG_5790/MSMEI_5637"}}},"genes":[{"orderedLocusNames":[{"value":"MSMEG_5790"},{"value":"MSMEI_5637"}]}],"comments":[{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AFP42072.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"Uncharacterized protein MSMEG_5790/MSMEI_5637","featureId":"PRO_0000396089"},{"type":"Cross-link","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20094657"}]}],"keywords":[{"id":"KW-1017","category":"PTM","name":"Isopeptide bond"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-ET8TSAB7T4FCA","citationType":"submission","authors":["Fleischmann R.D.","Dodson R.J.","Haft D.H.","Merkel J.S.","Nelson W.C.","Fraser C.M."],"publicationDate":"OCT-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"17295914","citationType":"journal article","authors":["Deshayes C.","Perrodou E.","Gallien S.","Euphrasie D.","Schaeffer C.","Van-Dorsselaer A.","Poch O.","Lecompte O.","Reyrat J.-M."],"citationCrossReferences":[{"database":"PubMed","id":"17295914"},{"database":"DOI","id":"10.1186/gb-2007-8-2-r20"}],"title":"Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?","publicationDate":"2007","journal":"Genome Biol.","firstPage":"R20.1","lastPage":"R20.9","volume":"8"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"18955433","citationType":"journal article","authors":["Gallien S.","Perrodou E.","Carapito C.","Deshayes C.","Reyrat J.-M.","Van Dorsselaer A.","Poch O.","Schaeffer C.","Lecompte O."],"citationCrossReferences":[{"database":"PubMed","id":"18955433"},{"database":"DOI","id":"10.1101/gr.081901.108"}],"title":"Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol.","publicationDate":"2009","journal":"Genome Res.","firstPage":"128","lastPage":"135","volume":"19"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700084 / mc(2)155","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"20094657","citationType":"journal article","authors":["Watrous J.","Burns K.","Liu W.T.","Patel A.","Hook V.","Bafna V.","Barry C.E. III","Bark S.","Dorrestein P.C."],"citationCrossReferences":[{"database":"PubMed","id":"20094657"},{"database":"DOI","id":"10.1039/b916104j"}],"title":"Expansion of the mycobacterial 'PUPylome'.","publicationDate":"2010","journal":"Mol. Biosyst.","firstPage":"376","lastPage":"385","volume":"6"},"referencePositions":["PUPYLATION AT LYS-88","IDENTIFICATION BY MASS SPECTROMETRY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000480","properties":[{"key":"ProteinId","value":"ABK70882.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP001663","properties":[{"key":"ProteinId","value":"AFP42072.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0R4D0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0R4D0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"246196.MSMEG_5790","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"246196-MSMEG_5790","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"msg:MSMEI_5637","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"msm:MSMEG_5790","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|246196.19.peg.5635","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"ENOG5032Y6I","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"OrthoDB","id":"3729294at2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000757","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"Gene3D","id":"2.60.40.1120","properties":[{"key":"EntryName","value":"Carboxypeptidase-like, regulatory domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008969","properties":[{"key":"EntryName","value":"CarboxyPept-like_regulatory"}]},{"database":"InterPro","id":"IPR010814","properties":[{"key":"EntryName","value":"DUF1416"}]},{"database":"Pfam","id":"PF07210","properties":[{"key":"EntryName","value":"DUF1416"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF49464","properties":[{"key":"EntryName","value":"Carboxypeptidase regulatory domain-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPAGVDLEKETVITGRVVDGSGQAVGGAFVRLLDGSDEFTAEVVASATGDFRFFAAPGTWTVRALSSAGNGNVTVAPTGAGIHEVDVKVA","length":90,"molWeight":9050,"crc64":"BC3B8F7C66EBAEEF","md5":"5629A9B68565AE5927EC6D5506F83747"},"extraAttributes":{"countByCommentType":{"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Cross-link":1},"uniParcId":"UPI0000E85EF0"}}