{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A8I3MKU8","uniProtkbId":"TM147_CANLF","entryAudit":{"firstPublicDate":"2023-02-22","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2022-05-25","entryVersion":18,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Canis lupus familiaris","commonName":"Dog","synonyms":["Canis familiaris"],"taxonId":9615,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Laurasiatheria","Carnivora","Caniformia","Canidae","Canis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"BOS complex subunit TMEM147"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Transmembrane protein 147"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"36261528"}],"value":"TMEM147"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BVK8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36261528"}],"value":"Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:36261528). The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed:36261528). Also acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (By similarity). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (By similarity). Regulates LBR localization to the nucleus inner membrane (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BVK8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36261528"}],"value":"Component of the back of Sec61 (BOS) complex, composed of NCLN/Nicalin, NOMO1 and TMEM147 (PubMed:36261528). The BOS complex is part of the multi-pass translocon (MPT) complex, composed of three subcomplexes, the GEL complex (composed of RAB5IF/OPTI and TMCO1), the BOS complex (composed of NCLN/Nicalin, NOMO1 and TMEM147) and the PAT complex (composed of WDR83OS/Asterix and CCDC47) (PubMed:36261528). The MPT complex associates with the SEC61 complex (PubMed:36261528). Interacts with CHRM3, CHRM1 and AVPR2 (By similarity). Interacts with LBR; promoting LBR localization to the nucleus inner membrane (By similarity). Interacts with DHCR7 (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36261528"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36261528"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9BVK8"}],"value":"Nucleus membrane","id":"SL-0182"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36261528"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I6VSD2"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36261528"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the TMEM147 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"BOS complex subunit TMEM147","featureId":"PRO_0000457553"},{"type":"Transmembrane","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":21,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Transmembrane","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":59,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Transmembrane","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":89,"modifier":"EXACT"},"end":{"value":98,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Transmembrane","location":{"start":{"value":99,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Transmembrane","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Transmembrane","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":187,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":188,"modifier":"EXACT"},"end":{"value":194,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Transmembrane","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]},{"type":"Topological domain","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"36261528"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"16341006","citationType":"journal article","authors":["Lindblad-Toh K.","Wade C.M.","Mikkelsen T.S.","Karlsson E.K.","Jaffe D.B.","Kamal M.","Clamp M.","Chang J.L.","Kulbokas E.J. III","Zody M.C.","Mauceli E.","Xie X.","Breen M.","Wayne R.K.","Ostrander E.A.","Ponting C.P.","Galibert F.","Smith D.R.","deJong P.J.","Kirkness E.F.","Alvarez P.","Biagi T.","Brockman W.","Butler J.","Chin C.-W.","Cook A.","Cuff J.","Daly M.J.","DeCaprio D.","Gnerre S.","Grabherr M.","Kellis M.","Kleber M.","Bardeleben C.","Goodstadt L.","Heger A.","Hitte C.","Kim L.","Koepfli K.-P.","Parker H.G.","Pollinger J.P.","Searle S.M.J.","Sutter N.B.","Thomas R.","Webber C.","Baldwin J.","Abebe A.","Abouelleil A.","Aftuck L.","Ait-Zahra M.","Aldredge T.","Allen N.","An P.","Anderson S.","Antoine C.","Arachchi H.","Aslam A.","Ayotte L.","Bachantsang P.","Barry A.","Bayul T.","Benamara M.","Berlin A.","Bessette D.","Blitshteyn B.","Bloom T.","Blye J.","Boguslavskiy L.","Bonnet C.","Boukhgalter B.","Brown A.","Cahill P.","Calixte N.","Camarata J.","Cheshatsang Y.","Chu J.","Citroen M.","Collymore A.","Cooke P.","Dawoe T.","Daza R.","Decktor K.","DeGray S.","Dhargay N.","Dooley K.","Dooley K.","Dorje P.","Dorjee K.","Dorris L.","Duffey N.","Dupes A.","Egbiremolen O.","Elong R.","Falk J.","Farina A.","Faro S.","Ferguson D.","Ferreira P.","Fisher S.","FitzGerald M.","Foley K.","Foley C.","Franke A.","Friedrich D.","Gage D.","Garber M.","Gearin G.","Giannoukos G.","Goode T.","Goyette A.","Graham J.","Grandbois E.","Gyaltsen K.","Hafez N.","Hagopian D.","Hagos B.","Hall J.","Healy C.","Hegarty R.","Honan T.","Horn A.","Houde N.","Hughes L.","Hunnicutt L.","Husby M.","Jester B.","Jones C.","Kamat A.","Kanga B.","Kells C.","Khazanovich D.","Kieu A.C.","Kisner P.","Kumar M.","Lance K.","Landers T.","Lara M.","Lee W.","Leger J.-P.","Lennon N.","Leuper L.","LeVine S.","Liu J.","Liu X.","Lokyitsang Y.","Lokyitsang T.","Lui A.","Macdonald J.","Major J.","Marabella R.","Maru K.","Matthews C.","McDonough S.","Mehta T.","Meldrim J.","Melnikov A.","Meneus L.","Mihalev A.","Mihova T.","Miller K.","Mittelman R.","Mlenga V.","Mulrain L.","Munson G.","Navidi A.","Naylor J.","Nguyen T.","Nguyen N.","Nguyen C.","Nguyen T.","Nicol R.","Norbu N.","Norbu C.","Novod N.","Nyima T.","Olandt P.","O'Neill B.","O'Neill K.","Osman S.","Oyono L.","Patti C.","Perrin D.","Phunkhang P.","Pierre F.","Priest M.","Rachupka A.","Raghuraman S.","Rameau R.","Ray V.","Raymond C.","Rege F.","Rise C.","Rogers J.","Rogov P.","Sahalie J.","Settipalli S.","Sharpe T.","Shea T.","Sheehan M.","Sherpa N.","Shi J.","Shih D.","Sloan J.","Smith C.","Sparrow T.","Stalker J.","Stange-Thomann N.","Stavropoulos S.","Stone C.","Stone S.","Sykes S.","Tchuinga P.","Tenzing P.","Tesfaye S.","Thoulutsang D.","Thoulutsang Y.","Topham K.","Topping I.","Tsamla T.","Vassiliev H.","Venkataraman V.","Vo A.","Wangchuk T.","Wangdi T.","Weiand M.","Wilkinson J.","Wilson A.","Yadav S.","Yang S.","Yang X.","Young G.","Yu Q.","Zainoun J.","Zembek L.","Zimmer A.","Lander E.S."],"citationCrossReferences":[{"database":"PubMed","id":"16341006"},{"database":"DOI","id":"10.1038/nature04338"}],"title":"Genome sequence, comparative analysis and haplotype structure of the domestic dog.","publicationDate":"2005","journal":"Nature","firstPage":"803","lastPage":"819","volume":"438"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"Boxer","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"36261528","citationType":"journal article","authors":["Smalinskaite L.","Kim M.K.","Lewis A.J.O.","Keenan R.J.","Hegde R.S."],"citationCrossReferences":[{"database":"PubMed","id":"36261528"},{"database":"DOI","id":"10.1038/s41586-022-05336-2"}],"title":"Mechanism of an intramembrane chaperone for multipass membrane proteins.","publicationDate":"2022","journal":"Nature","firstPage":"161","lastPage":"166","volume":"611"},"referencePositions":["STRUCTURE BY ELECTRON MICROSCOPY (3.88 ANGSTROMS) IN COMPLEX WITH THE MULTI-PASS TRANSLOCON COMPLEX","FUNCTION","IDENTIFICATION IN THE MULTI-PASS TRANSLOCON COMPLEX","SUBCELLULAR LOCATION"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TM3"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"7TUT"}]}],"uniProtKBCrossReferences":[{"database":"RefSeq","id":"XP_038381607.1","properties":[{"key":"NucleotideSequenceId","value":"XM_038525679.1"}]},{"database":"RefSeq","id":"XP_038513372.1","properties":[{"key":"NucleotideSequenceId","value":"XM_038657444.1"}]},{"database":"RefSeq","id":"XP_533692.1","properties":[{"key":"NucleotideSequenceId","value":"XM_533692.7"}]},{"database":"PDB","id":"7TM3","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.88 A"},{"key":"Chains","value":"6=1-224"}]},{"database":"PDB","id":"7TUT","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.88 A"},{"key":"Chains","value":"6=1-224"}]},{"database":"PDBsum","id":"7TM3","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"7TUT","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"A0A8I3MKU8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A8I3MKU8","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"A0A8I3MKU8","properties":[{"key":"Number of interactors","value":"2070"}]},{"database":"Ensembl","id":"ENSCAFT00030009720","properties":[{"key":"ProteinId","value":"ENSCAFP00030008501"},{"key":"GeneId","value":"ENSCAFG00030005302"}]},{"database":"Ensembl","id":"ENSCAFT00040001168","properties":[{"key":"ProteinId","value":"ENSCAFP00040000990"},{"key":"GeneId","value":"ENSCAFG00040000656"}]},{"database":"Ensembl","id":"ENSCAFT00845004203.1","properties":[{"key":"ProteinId","value":"ENSCAFP00845003360.1"},{"key":"GeneId","value":"ENSCAFG00845002384.1"}]},{"database":"GeneID","id":"476482","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"cfa:476482","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"10430","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00390000013276","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"SKCVYAG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9993532at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:A0A8I3MKU8","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000805418","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36261528"}]},{"database":"GO","id":"GO:0160064","properties":[{"key":"GoTerm","value":"C:multi-pass translocon complex"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36261528"}]},{"database":"GO","id":"GO:0031965","properties":[{"key":"GoTerm","value":"C:nuclear membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0043022","properties":[{"key":"GoTerm","value":"F:ribosome binding"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0160063","properties":[{"key":"GoTerm","value":"P:multi-pass transmembrane protein insertion into ER membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"36261528"}]},{"database":"GO","id":"GO:0036228","properties":[{"key":"GoTerm","value":"P:protein localization to nuclear inner membrane"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"InterPro","id":"IPR019164","properties":[{"key":"EntryName","value":"TMEM147"}]},{"database":"PANTHER","id":"PTHR12869:SF0","properties":[{"key":"EntryName","value":"BOS COMPLEX SUBUNIT TMEM147"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12869","properties":[{"key":"EntryName","value":"SMALL SEVEN TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09767","properties":[{"key":"EntryName","value":"DUF2053"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MTLFHFGNCFALAYFPYFITYKCSGLSEYNAFWKCVQAGVTYLFVQLCKMLFLATFFPTWEGGIYDFIGEFMKASVDVADLIGLNLVMSRNAGKGEYKIMVAALGWATAELIMSRCIPLWVGARGIEFDWKYIQMSIDSNISLVHYIVASAQVWMITRYDLYHTFRPAVLLLMFLSVYKAFVMETFVHLCSLGSWTALLARAVVTGLLALSTLALYVAVVNVHS","length":224,"molWeight":25291,"crc64":"90122F32D71E53AE","md5":"6CD1A10FF0C8718C115CB1AEB6CDFD46"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":7,"Topological domain":7},"uniParcId":"UPI0000181B67"}}