{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A7H0DNF0","uniProtkbId":"POXIN_MONPV","entryAudit":{"firstPublicDate":"2023-02-22","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2022-10-12","entryVersion":14,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Monkeypox virus","commonName":"MPXV","taxonId":10244,"lineage":["Viruses","Varidnaviria","Bamfordvirae","Nucleocytoviricota","Pokkesviricetes","Chitovirales","Poxviridae","Chordopoxvirinae","Orthopoxvirus","Orthopoxvirus monkeypox"]},"organismHosts":[{"scientificName":"Cynomys gunnisoni","commonName":"Gunnison's prairie dog","synonyms":["Spermophilus gunnisoni"],"taxonId":45479},{"scientificName":"Cynomys leucurus","commonName":"White-tailed prairie dog","taxonId":99825},{"scientificName":"Cynomys ludovicianus","commonName":"Black-tailed prairie dog","taxonId":45480},{"scientificName":"Cynomys mexicanus","commonName":"Mexican prairie dog","taxonId":99826},{"scientificName":"Cynomys parvidens","commonName":"Utah prairie dog","taxonId":99827},{"scientificName":"Gliridae","commonName":"dormice","taxonId":30650},{"scientificName":"Heliosciurus ruwenzorii","commonName":"Ruwenzori sun squirrel","taxonId":226685},{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606},{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}],"value":"Poxin-Schlafen"}}},"genes":[{"geneName":{"value":"OPG188"},"orfNames":[{"value":"MPXVgp165"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}],"value":"Nuclease that is responsible for viral evasion of host cGAS-STING innate immunity. Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of cytosolic DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING, which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2',3'-cGAMP + H2O = Gp(2'-5')Ap(3') + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59472"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:143093"},{"database":"ChEBI","id":"CHEBI:143098"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59473"},"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"value":"Expressed in the early phase of the viral replicative cycle"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}],"value":"The substrate binding site is formed by the N-terminus of a monomer and the C-terminus of the opposite monomer"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}],"value":"In the N-terminal section; belongs to the poxin family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}],"value":"In the C-terminal section; belongs to the Schlafen protein family. Subgroup poxviridae B3 subfamily"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":503,"modifier":"EXACT"}},"description":"Poxin-Schlafen","featureId":"PRO_0000457612"},{"type":"Active site","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Active site","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":136,"modifier":"EXACT"}},"description":"Shared with catalytic histidine of dimeric partner","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Active site","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Proton acceptor; shared with catalytic histidine of dimeric partner","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":180,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Site","location":{"start":{"value":184,"modifier":"EXACT"},"end":{"value":184,"modifier":"EXACT"}},"description":"Substrate binding","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_04143"}]},{"type":"Beta strand","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Helix","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":14,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":20,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Helix","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":55,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":58,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":78,"modifier":"EXACT"},"end":{"value":80,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":86,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":129,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":132,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Helix","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":156,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":171,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]},{"type":"Beta strand","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":183,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"8ORV"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0244","category":"Developmental stage","name":"Early protein"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"32880628","citationType":"journal article","authors":["Mauldin M.R.","McCollum A.M.","Nakazawa Y.J.","Mandra A.","Whitehouse E.R.","Davidson W.","Zhao H.","Gao J.","Li Y.","Doty J.","Yinka-Ogunleye A.","Akinpelu A.","Aruna O.","Naidoo D.","Lewandowski K.","Afrough B.","Graham V.","Aarons E.","Hewson R.","Vipond R.","Dunning J.","Chand M.","Brown C.","Cohen-Gihon I.","Erez N.","Shifman O.","Israeli O.","Sharon M.","Schwartz E.","Beth-Din A.","Zvi A.","Mak T.M.","Ng Y.K.","Cui L.","Lin R.T.P.","Olson V.A.","Brooks T.","Paran N.","Ihekweazu C.","Reynolds M.G."],"citationCrossReferences":[{"database":"PubMed","id":"32880628"},{"database":"DOI","id":"10.1093/infdis/jiaa559"}],"title":"Exportation of Monkeypox virus from the African continent.","publicationDate":"2022","journal":"J. Infect. Dis.","firstPage":"1367","lastPage":"1376","volume":"225"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"MPXV-M5312_HM12_Rivers","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MT903340","properties":[{"key":"ProteinId","value":"QNP13033.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"YP_010377160.1","properties":[{"key":"NucleotideSequenceId","value":"NC_063383.1"}]},{"database":"PDB","id":"8C9K","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.72 A"},{"key":"Chains","value":"A/B/C/D=1-195"}]},{"database":"PDB","id":"8ORV","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.65 A"},{"key":"Chains","value":"A=1-195"}]},{"database":"PDB","id":"8P44","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.93 A"},{"key":"Chains","value":"A/B/C/D=1-195"}]},{"database":"PDBsum","id":"8C9K","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8ORV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"8P44","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"A0A7H0DNF0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A7H0DNF0","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"72551574","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vg:72551574","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000516359","properties":[{"key":"Component","value":"Segment"}]},{"database":"GO","id":"GO:0061507","properties":[{"key":"GoTerm","value":"F:2',3'-cyclic GMP-AMP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0016787","properties":[{"key":"GoTerm","value":"F:hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0004518","properties":[{"key":"GoTerm","value":"F:nuclease activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0052170","properties":[{"key":"GoTerm","value":"P:symbiont-mediated suppression of host innate immune response"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"Gene3D","id":"3.30.950.30","properties":[{"key":"EntryName","value":"Schlafen, AAA domain"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_04143","properties":[{"key":"EntryName","value":"Poxins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR031450","properties":[{"key":"EntryName","value":"Poxin-SLFN/SLFN_N"}]},{"database":"InterPro","id":"IPR006853","properties":[{"key":"EntryName","value":"Poxin_vir"}]},{"database":"InterPro","id":"IPR029684","properties":[{"key":"EntryName","value":"Schlafen"}]},{"database":"InterPro","id":"IPR007421","properties":[{"key":"EntryName","value":"Schlafen_AlbA_2_dom"}]},{"database":"InterPro","id":"IPR038461","properties":[{"key":"EntryName","value":"Schlafen_AlbA_2_dom_sf"}]},{"database":"PANTHER","id":"PTHR12155:SF2","properties":[{"key":"EntryName","value":"RIBONUCLEASE SLFN12"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12155","properties":[{"key":"EntryName","value":"SCHLAFEN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF17057","properties":[{"key":"EntryName","value":"B3R"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04766","properties":[{"key":"EntryName","value":"Baculo_p26"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04326","properties":[{"key":"EntryName","value":"SLFN_AlbA_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFYAHAFGGYDENLHAFPGISSTVANDVRKYSVVSVYNKKYNIVKNKYMWCNSQVNKRYIGALLPMFECNEYLQIGDPIHDLEGNQISIVTYRHKNYYALSGIGYESLDLCLEGVGIHHHVLETGNAVYGKVQHEYSTIKEKAKEMNALKPGPIIDYHVWIGDCVCQVTTVDVHGKEIMRMRFKRGAVLPIPNLVKVKVGEENDTINLSTSISALLNSGGGTIEVTSKEERVDYVLMKRLESIHHLWSVVYDHLNVVNGEERCYVHMHSSHQSPMLSTVKTNLYMKTMGACLQMDSMEALEYLSELKESGGRSPRPELQKFEYPDGVKDTESIERLAEEFFNRSELQAGESVKFGNSINVKHTSVSAKQLRTRIRQQLPSILSSFANTKGGYLFIGVDNNTHKVIGFTVGHDYLKLVESDIEKYIQKLPVVHFCKKKEDIKYACRFIKVYKPGDETTSTYVCAIKVERCCCAVFADWPESWYMDTSGSMKKYSPDEWVSHIKF","length":503,"molWeight":57272,"crc64":"3F17619FDBB0CC67","md5":"E0548A7E9E2DE78B93694418A64FA4DA"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"INDUCTION":1,"DOMAIN":1,"SIMILARITY":2},"countByFeatureType":{"Chain":1,"Active site":3,"Site":7,"Beta strand":18,"Helix":3},"uniParcId":"UPI00062AE300"}}