{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A571BE98","uniProtkbId":"LSME2_MOUSE","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2019-10-16","entryVersion":27,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Leucine-rich single-pass membrane protein 2"}}},"genes":[{"geneName":{"value":"Lsmem2"}}],"comments":[{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40525396"}],"value":"In skeletal muscles, localizes at neuromuscular junction"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40525396"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40525396"}],"value":"Expressed in heart (PubMed:40525396). Expressed in skeletal muscles (at protein level) (PubMed:40525396)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"40525396"}],"value":"Knockout mice do not show any developmental abnormalities, fertility defects or histologic abnormalities"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Leucine-rich single-pass membrane protein 2","featureId":"PRO_0000463700"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N112"}]},{"type":"Transmembrane","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N112"}]},{"type":"Glycosylation","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N112"}],"featureId":""}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"19468303","citationType":"journal article","authors":["Church D.M.","Goodstadt L.","Hillier L.W.","Zody M.C.","Goldstein S.","She X.","Bult C.J.","Agarwala R.","Cherry J.L.","DiCuccio M.","Hlavina W.","Kapustin Y.","Meric P.","Maglott D.","Birtle Z.","Marques A.C.","Graves T.","Zhou S.","Teague B.","Potamousis K.","Churas C.","Place M.","Herschleb J.","Runnheim R.","Forrest D.","Amos-Landgraf J.","Schwartz D.C.","Cheng Z.","Lindblad-Toh K.","Eichler E.E.","Ponting C.P."],"citationCrossReferences":[{"database":"PubMed","id":"19468303"},{"database":"DOI","id":"10.1371/journal.pbio.1000112"}],"title":"Lineage-specific biology revealed by a finished genome assembly of the mouse.","publicationDate":"2009","journal":"PLoS Biol.","firstPage":"E1000112","lastPage":"E1000112","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"40525396","citationType":"journal article","authors":["Elrefaei E.","Yamazaki S.","Yazawa I.","Takahashi Y.","Ito N.","Hayashiji N.","Nishida Y.","Nishino I.","Takashima S.","Shintani Y."],"citationCrossReferences":[{"database":"PubMed","id":"40525396"},{"database":"DOI","id":"10.1096/fj.202402152r"}],"title":"LSMEM2, Localized at the Neuromuscular Junction, Modulates Mitochondrial Integration in Skeletal Muscles.","publicationDate":"2025","journal":"FASEB J.","firstPage":"e70609","lastPage":"e70609","volume":"39"},"referencePositions":["SUBCELLULAR LOCATION","TISSUE SPECIFICITY","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"MU069431","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"NP_001357806.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001370877.1"}]},{"database":"AlphaFoldDB","id":"A0A571BE98","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A571BE98","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"A0A571BE98","properties":[{"key":"Number of interactors","value":"19"}]},{"database":"STRING","id":"10090.ENSMUSP00000158647","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000158647","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"64735","properties":[{"key":"antibodies","value":"28 antibodies from 7 providers"}]},{"database":"GeneID","id":"434436","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:434436","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"MGI:3612240","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"132228","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:3612240","properties":[{"key":"GeneName","value":"Lsmem2"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000103409","properties":[{"key":"Description","value":"-"}]},{"database":"GeneTree","id":"ENSGT00390000000577","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"A0A571BE98","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AHTLHTQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9447266at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 9"}]},{"database":"Bgee","id":"ENSMUSG00000103409","properties":[{"key":"ExpressionPatterns","value":"Expressed in quadriceps femoris and 53 other cell types or tissues"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"InterPro","id":"IPR031674","properties":[{"key":"EntryName","value":"DUF4714"}]},{"database":"PANTHER","id":"PTHR37333","properties":[{"key":"EntryName","value":"LEUCINE-RICH SINGLE-PASS MEMBRANE PROTEIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37333:SF1","properties":[{"key":"EntryName","value":"LEUCINE-RICH SINGLE-PASS MEMBRANE PROTEIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF15833","properties":[{"key":"EntryName","value":"DUF4714"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPEETLEDTVLPMQNQRNRGALAPNHVQEVRLHRVESISDLHSGGSLRPCLVEEAQAWEELLGVLPPSLCTQAGCSPVCGRGGFLLLLVLLVFTCLALATVAVYLSVLQSESLRVLAHTLRTQEETLLKLRLASLSQLRRLNSSEARAPS","length":150,"molWeight":16405,"crc64":"6F050908532408F7","md5":"EFFC8C89045858DDB851A59E40FB69E1"},"extraAttributes":{"countByCommentType":{"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Topological domain":2,"Transmembrane":1,"Glycosylation":1},"uniParcId":"UPI0002507980"}}