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<entry dataset="TrEMBL" created="2019-07-31" modified="2025-04-02" version="20" xmlns="http://uniprot.org/uniprot">
  <accession>A0A4R7PEM6</accession>
  <name>A0A4R7PEM6_9GAMM</name>
  <protein>
    <submittedName>
      <fullName evidence="5">Aliphatic nitrilase</fullName>
    </submittedName>
  </protein>
  <gene>
    <name evidence="5" type="ORF">DFR24_2065</name>
  </gene>
  <organism evidence="5 6">
    <name type="scientific">Panacagrimonas perspica</name>
    <dbReference type="NCBI Taxonomy" id="381431"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Nevskiales</taxon>
      <taxon>Nevskiaceae</taxon>
      <taxon>Panacagrimonas</taxon>
    </lineage>
  </organism>
  <reference evidence="5 6" key="1">
    <citation type="submission" date="2019-03" db="EMBL/GenBank/DDBJ databases">
      <title>Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the most valuable type-strain genomes for metagenomic binning, comparative biology and taxonomic classification.</title>
      <authorList>
        <person name="Goeker M."/>
      </authorList>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain evidence="5 6">DSM 26377</strain>
    </source>
  </reference>
  <comment type="similarity">
    <text evidence="1">Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.</text>
  </comment>
  <comment type="caution">
    <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
  </comment>
  <dbReference type="EMBL" id="SOBT01000008">
    <property type="protein sequence ID" value="TDU32665.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_133881149.1">
    <property type="nucleotide sequence ID" value="NZ_MWIN01000001.1"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="A0A4R7PEM6"/>
  <dbReference type="OrthoDB" id="9803803at2"/>
  <dbReference type="Proteomes" id="UP000295341">
    <property type="component" value="Unassembled WGS sequence"/>
  </dbReference>
  <dbReference type="GO" id="GO:0000257">
    <property type="term" value="F:nitrilase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-ARBA"/>
  </dbReference>
  <dbReference type="CDD" id="cd07564">
    <property type="entry name" value="nitrilases_CHs"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.60.110.10">
    <property type="entry name" value="Carbon-nitrogen hydrolase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR003010">
    <property type="entry name" value="C-N_Hydrolase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR036526">
    <property type="entry name" value="C-N_Hydrolase_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR000132">
    <property type="entry name" value="Nitrilase/CN_hydratase_CS"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR044149">
    <property type="entry name" value="Nitrilases_CHs"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR46044:SF1">
    <property type="entry name" value="CN HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR46044">
    <property type="entry name" value="NITRILASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF00795">
    <property type="entry name" value="CN_hydrolase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF56317">
    <property type="entry name" value="Carbon-nitrogen hydrolase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS50263">
    <property type="entry name" value="CN_HYDROLASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS00920">
    <property type="entry name" value="NITRIL_CHT_1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
  <feature type="domain" description="CN hydrolase" evidence="4">
    <location>
      <begin position="9"/>
      <end position="282"/>
    </location>
  </feature>
  <feature type="region of interest" description="Disordered" evidence="3">
    <location>
      <begin position="327"/>
      <end position="366"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Low complexity" evidence="3">
    <location>
      <begin position="327"/>
      <end position="356"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
    <location>
      <begin position="357"/>
      <end position="366"/>
    </location>
  </feature>
  <feature type="active site" description="Proton acceptor" evidence="2">
    <location>
      <position position="49"/>
    </location>
  </feature>
  <evidence type="ECO:0000256" key="1">
    <source>
      <dbReference type="ARBA" id="ARBA00008129"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="2">
    <source>
      <dbReference type="PROSITE-ProRule" id="PRU10139"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="3">
    <source>
      <dbReference type="SAM" id="MobiDB-lite"/>
    </source>
  </evidence>
  <evidence type="ECO:0000259" key="4">
    <source>
      <dbReference type="PROSITE" id="PS50263"/>
    </source>
  </evidence>
  <evidence type="ECO:0000313" key="5">
    <source>
      <dbReference type="EMBL" id="TDU32665.1"/>
    </source>
  </evidence>
  <evidence type="ECO:0000313" key="6">
    <source>
      <dbReference type="Proteomes" id="UP000295341"/>
    </source>
  </evidence>
  <sequence length="366" mass="39495" checksum="E37BFA6D20CBCFFE" modified="2019-07-31" version="1">MPAPNHPRLRVAAVQAVPEFLDLDGTIDKTIALMEQASKQGAQLVAFPETWIPGYPWWIWLNPPALGMQYVQRYFDHSLVVGSDEFKRLADAARSLKIWLALGYSERAGGSLYIAQALIDAEGRTQMTRRKLRPTHVERTVFGDGDGSDLHVVQTSVGNVGMLNCWEHLQPLSKYALYSQNEQIHIGAWPSFSIYPGKAHALGPEVNTAASRVYAAEGQCFVVAPCAVVSPQMHAMLCTDELQRQLLRPGGGYARIYGPDGAELGTSLAPDEEGLVLADIDLGTISLAKHAADPVGHYARPDVTRLLLNKTPGDRVVSWVDNANAQGGAEASATRAAQGARTTAPGRSLPSAPDSADAARREPDQA</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>