{"entryType":"UniProtKB unreviewed (TrEMBL)","primaryAccession":"A0A432WN50","uniProtkbId":"A0A432WN50_9GAMM","entryAudit":{"firstPublicDate":"2019-05-08","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2019-05-08","entryVersion":15,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Aliidiomarina sanyensis","taxonId":1249555,"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"RUO35226.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000288405"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Alteromonadales","Idiomarinaceae","Aliidiomarina"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52035"}],"value":"Peptidase M14 domain-containing protein"}}},"genes":[{"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"RUO35226.1"}],"value":"CWE11_04105"}]}],"comments":[{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00001947"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01379"}],"value":"Belongs to the peptidase M14 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"RUO35226.1"}],"value":"The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data"}],"commentType":"CAUTION"}],"features":[{"type":"Transmembrane","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":74,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Transmembrane","location":{"start":{"value":114,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}]},{"type":"Domain","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":460,"modifier":"EXACT"}},"description":"Peptidase M14","evidences":[{"evidenceCode":"ECO:0000259","source":"PROSITE","id":"PS52035"}]},{"type":"Active site","location":{"start":{"value":430,"modifier":"EXACT"},"end":{"value":430,"modifier":"EXACT"}},"description":"Proton donor/acceptor","evidences":[{"evidenceCode":"ECO:0000256","source":"PROSITE-ProRule","id":"PRU01379"}]}],"keywords":[{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022801"}],"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022723"}],"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"evidences":[{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000288405"}],"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"Phobius"}],"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"evidences":[{"evidenceCode":"ECO:0000256","source":"ARBA","id":"ARBA00022833"}],"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"21464989","citationType":"journal article","authors":["Gibbons H.S.","Broomall S.M.","McNew L.A.","Daligault H.","Chapman C.","Bruce D.","Karavis M.","Krepps M.","McGregor P.A.","Hong C.","Park K.H.","Akmal A.","Feldman A.","Lin J.S.","Chang W.E.","Higgs B.W.","Demirev P.","Lindquist J.","Liem A.","Fochler E.","Read T.D.","Tapia R.","Johnson S.","Bishop-Lilly K.A.","Detter C.","Han C.","Sozhamannan S.","Rosenzweig C.N.","Skowronski E.W."],"citationCrossReferences":[{"database":"PubMed","id":"21464989"},{"database":"DOI","id":"10.1371/journal.pone.0017836"}],"title":"Genomic signatures of strain selection and enhancement in Bacillus atrophaeus var. globigii, a historical biowarfare simulant.","publicationDate":"2011","journal":"Front. Microbiol.","firstPage":"E17836","lastPage":"E17836","volume":"6"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"RUO35226.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000288405"}],"value":"GYP-17","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000313","source":"EMBL","id":"RUO35226.1"},{"evidenceCode":"ECO:0000313","source":"Proteomes","id":"UP000288405"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"PIPM01000003","properties":[{"key":"ProteinId","value":"RUO35226.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A432WN50","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"5290048at2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000288405","properties":[{"key":"Component","value":"Unassembled WGS sequence"}]},{"database":"GO","id":"GO:0016788","properties":[{"key":"GoTerm","value":"F:hydrolase activity, acting on ester bonds"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004181","properties":[{"key":"GoTerm","value":"F:metallocarboxypeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0008270","properties":[{"key":"GoTerm","value":"F:zinc ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd06231","properties":[{"key":"EntryName","value":"M14_REP34-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.630.10","properties":[{"key":"EntryName","value":"Zn peptidases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR055438","properties":[{"key":"EntryName","value":"AstE_AspA_cat"}]},{"database":"InterPro","id":"IPR021218","properties":[{"key":"EntryName","value":"DUF2784"}]},{"database":"InterPro","id":"IPR000834","properties":[{"key":"EntryName","value":"Peptidase_M14"}]},{"database":"Pfam","id":"PF24827","properties":[{"key":"EntryName","value":"AstE_AspA_cat"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10861","properties":[{"key":"EntryName","value":"DUF2784"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53187","properties":[{"key":"EntryName","value":"Zn-dependent exopeptidases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52035","properties":[{"key":"EntryName","value":"PEPTIDASE_M14"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSKINEIPTWTQMVPFPGLAADIVMIIHALIVVFVVLSLPLTIVGGYRRWHWIRNSWFRTIHFAIIVVVVIQSFSGRYCPLTYVEQDLRLAAGQPSFDSSFIDYWVSRFIYFDLPAWIFMATYALFFLAVVYTWWRWPPRMLAARRRYESRLYMKFSEGYPIGSPGIPWGDNEKAAWLRKQRKRRSYTQDVVSRIDALRAHYDVEVYGVLPYSEQVGTDYELFAVRSKNWLDSRPTILVTGGVHGYETSGVLGALAFLETVAAQYQAHFNVLVLPCISPWGYETINRWNPDAVDPNRSFTEDAPAQEAQLAMAYVAKHAPELLMHIDLHETTDTDNTEFRPALAARDGTVNTNWNIPDGFYLVGDTERPTPDFQKAILNSVKKVTHIAEADERNELIGVPVEQFGVIHYPGKQAGLCMGLSDAPYVSTTEVYPDSANATPQECIDAQVAAIVGGLNYLQR","length":460,"molWeight":52207,"crc64":"932184E975E52A47","md5":"332DAEE8337CEE871CAD2C2C8C791265"},"extraAttributes":{"countByCommentType":{"COFACTOR":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Transmembrane":3,"Domain":1,"Active site":1},"uniParcId":"UPI000F87FA54"}}