{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A286LEZ6","uniProtkbId":"PSIK_PSICY","entryAudit":{"firstPublicDate":"2018-12-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-11-22","entryVersion":17,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Psilocybe cyanescens","taxonId":93625,"lineage":["Eukaryota","Fungi","Dikarya","Basidiomycota","Agaricomycotina","Agaricomycetes","Agaricomycetidae","Agaricales","Agaricineae","Strophariaceae","Psilocybe"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28763571"}],"value":"4-hydroxytryptamine kinase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35583969"}],"value":"2.7.1.-"},{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35583969"}],"value":"2.7.1.222"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28763571"}],"value":"Psilocybin biosynthesis kinase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28763571"}],"value":"psiK"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DPA8"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28763571"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30283667"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35583969"}],"value":"4-hydroxytryptamine kinase; part of the gene cluster that mediates the biosynthesis of psilocybin, a psychotropic tryptamine-derived natural product (PubMed:28763571, PubMed:30283667). The first step in the pathway is the decarboxylation of L-tryptophan to tryptamine by the decarboxylase psiD. PsiD does not decarboxylate phenylalanine, tyrosine, or 5-hydroxy- L -tryptophan (5-HTP) (PubMed:30283667). 4-hydroxy-L-tryptophan is accepted as substrate by psiD as well. The cytochrome P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine. The kinase psiK catalyzes the 4-O-phosphorylation step by converting 4-hydroxytryptamine into norbaeocystin. The methyltransferase psiM then catalyzes iterative methyl transfer to the amino group of norbaeocystin to yield psilocybin via a monomethylated intermediate, baeocystin. 4-hydroxy-6-methyl-l-tryptophancan also be converted the decarboxylase PsiD, kinase PsiK, and methyltransferase PsiM into respectively 6-methyl-norbaeocystin, 6-methylbaeocystin, and 6-methylpsilocybin (By similarity). PsiK kinase can also turn psilocin into psilocybin (PubMed:35583969). This activity may represent a protective mechanism to rephosphorylate the unstable psilocin to the stable psilocybin in case of intracellular ester cleavage (PubMed:35583969). Moreover, psiK is able to O-phosphorylate the quaternary amine 4-hydroxy-N,N,N-trimethyltryptamine (4-OH-TMT) to yield aeruginascin, another bioactive compound found in Psilocybe species (PubMed:35583969)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-hydroxytryptamine + ATP = norbaeocystin + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:55564"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:139069"},{"database":"ChEBI","id":"CHEBI:139070"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.222","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DPA8"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:55565"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DPA8"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"psilocin + ATP = 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