{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A1I1NU27","uniProtkbId":"BILB_COLS4","entryAudit":{"firstPublicDate":"2025-04-09","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2017-11-22","entryVersion":12,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Collimonas sp. (strain OK412)","taxonId":1801619,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Betaproteobacteria","Burkholderiales","Oxalobacteraceae","Collimonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"39020165"}],"value":"Bacterial E2-like ubiquitin protein BilB"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"39020165"}],"value":"2.3.2.-"}]},"alternativeNames":[{"fullName":{"value":"E2 ubiquitin-conjugating enzyme BilB"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"39020165"}],"value":"bilB"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"SFD01174.1"}],"value":"SAMN04515619_11957"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39020165"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"39020165"}],"value":"Component of the Bil (bacterial ISG15-like) antiviral defense system, composed of BilA, BilB, BilC and BilD. The Bil system specifically conjugates a ubiquitin-like moiety (bilA) to the bacteriophage central tail fiber (CTF, or tip attachment protein J) via reactions involving E1 (bilD) and E2 (bilB). Modifies CTF of phage SECphi27 and SECphi4, which probably interferes with assembly of the phage tail. Also modifies T5 baseplate hub protein pb3 (gene D16), but not gp27 of phage T6 (Bil defends against T6). BilB probably accepts ubiquitin from the BilA-BilD (E1) complex and catalyzes its covalent attachment to target protein (CTF) (Probable) (PubMed:39020165). Bil-encoding bacteria produce mostly defective phage SECphi27, many of which have phage assembly defects, including no tails. SECphi27 phage progeny produced in E.coli with the Bil system inject less DNA into naive host cells, maybe because the phage are less able to adsorb and inject their DNA into host cells"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39020165"}],"value":"Expression of the Bil system in E.coli (strain MG1655) confers about 100-fold resistance to phage SECphi27, SECphi18, SECphi6, SECphi4 and T5, but not to SECphi17. When cells expressing the Bil system are infected by phage SECphi27 at low multiplicity of infection (0.03 MOI) the culture survives, at 3.0 MOI the culture collapses at the same time as cells without the Bil system"}],"commentType":"FUNCTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Bacterial E2-like ubiquitin protein BilB","featureId":"PRO_0000462059"},{"type":"Active site","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Glycyl thioester intermediate","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"39020165"}]},{"type":"Mutagenesis","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"No longer resistant to SECphi27.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39020165"}],"alternativeSequence":{"originalSequence":"C","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0833","category":"Biological process","name":"Ubl conjugation pathway"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-EJS2D593375GF","citationType":"submission","authors":["de Groot N.N."],"publicationDate":"OCT-2016","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"OK412","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"SFD01174.1"}]},{"referenceNumber":2,"citation":{"id":"39020165","citationType":"journal article","authors":["Hoer J.","Wolf S.G.","Sorek R."],"citationCrossReferences":[{"database":"PubMed","id":"39020165"},{"database":"DOI","id":"10.1038/s41586-024-07616-5"}],"title":"Bacteria conjugate ubiquitin-like proteins to interfere with phage assembly.","publicationDate":"2024","journal":"Nature","firstPage":"850","lastPage":"856","volume":"631"},"referencePositions":["FUNCTION","PROBABLE ACTIVE SITE","MUTAGENESIS OF CYS-113"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"FOLC01000019","properties":[{"key":"ProteinId","value":"SFD01174.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_175501071.1","properties":[{"key":"NucleotideSequenceId","value":"NZ_FOLC01000019.1"}]},{"database":"AlphaFoldDB","id":"A0A1I1NU27","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A1I1NU27","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"1801619.SAMN04515619_11957","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0019787","properties":[{"key":"GoTerm","value":"F:ubiquitin-like protein transferase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"39020165"}]},{"database":"GO","id":"GO:0051607","properties":[{"key":"GoTerm","value":"P:defense response to virus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"39020165"}]},{"database":"GO","id":"GO:0032446","properties":[{"key":"GoTerm","value":"P:protein modification by small protein conjugation"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"39020165"}]},{"database":"InterPro","id":"IPR056082","properties":[{"key":"EntryName","value":"BilB-like"}]},{"database":"Pfam","id":"PF24702","properties":[{"key":"EntryName","value":"BilB-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MISAPSPDHLLLEQDLATPEIRCGEIEERWRHIRTSWPHVFFAVSAPQRPNGPKEFGFRFEYTGYRQTPVTAQLWDLEADAPLPHAKWPTGTYVVPSVFRKDWQQGNCLYIPCDRISIQGHTAWLNDHPSRLWQPARGIICYLEQLYELFNQGDYTGLVSA","length":161,"molWeight":18625,"crc64":"0FD68D3FC4904ECB","md5":"D82BA14B9AB4AA06BF8E83BDC40E72DD"},"extraAttributes":{"countByCommentType":{"FUNCTION":2},"countByFeatureType":{"Chain":1,"Active site":1,"Mutagenesis":1},"uniParcId":"UPI0008E218BF"}}