{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H3L116","uniProtkbId":"HILB_PANAA","entryAudit":{"firstPublicDate":"2022-02-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-09-16","entryVersion":33,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Pantoea ananatis (strain AJ13355)","taxonId":932677,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Erwiniaceae","Pantoea"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"3(1)-hydroxy-L-isoleucine 4-dioxygenase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23554367"}],"value":"1.14.11.75"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"4'-hydroxy-L-isoleucine 4-dioxygenase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"23554367"}],"value":"hilB"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"BAK13117.1"}],"value":"PAJ_3037"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22448874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23554367"}],"value":"Catalyzes the hydroxylation of L-4'-hydroxyisoleucine (4'-HIL) at the C-4 position to form L-4,4'-dihydroxyisoleucine (4,4'-DIHIL) (PubMed:23554367). Together with HilA, catalyzes the two step conversion of L-isoleucine into L-4,4'-dihydroxyisoleucine (PubMed:23554367). In vitro, in the absence of HilA, can also catalyze the oxidation of L-methionine and the C-4-hydroxylation of L-leucine and L-isoleucine (PubMed:22448874)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"3(1)-hydroxy-L-isoleucine + 2-oxoglutarate + O2 = (4S)-3(1),4-dihydroxy-L-isoleucine + succinate + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:63876"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:16810"},{"database":"ChEBI","id":"CHEBI:30031"},{"database":"ChEBI","id":"CHEBI:149627"},{"database":"ChEBI","id":"CHEBI:149628"}],"ecNumber":"1.14.11.75","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23554367"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:63877"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23554367"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"L-ascorbate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22448874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23554367"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:38290"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E2GIN1"}],"value":"Binds 1 ascorbate molecule per subunit."}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Fe(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22448874"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23554367"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29033"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"E2GIN1"}],"value":"Binds 1 Fe(2+) ion per subunit."}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":1.3,"unit":"mM","substrate":"L-methionine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22448874"}]},{"constant":1.1,"unit":"mM","substrate":"L-leucine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22448874"}]},{"constant":5.1,"unit":"mM","substrate":"L-isoleucine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22448874"}]}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the iron/ascorbate-dependent oxidoreductase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":247,"modifier":"EXACT"}},"description":"3(1)-hydroxy-L-isoleucine 4-dioxygenase","featureId":"PRO_0000454110"},{"type":"Binding site","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":160,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96323"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96323"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96323"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","note":"catalytic"}}],"keywords":[{"id":"KW-0223","category":"Molecular function","name":"Dioxygenase"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"},{"id":"KW-0847","category":"Ligand","name":"Vitamin C"}],"references":[{"referenceNumber":1,"citation":{"id":"22159605","citationType":"journal article","authors":["Hara Y.","Kadotani N.","Izui H.","Katashkina J.I.","Kuvaeva T.M.","Andreeva I.G.","Golubeva L.I.","Malko D.B.","Makeev V.J.","Mashko S.V.","Kozlov Y.I."],"citationCrossReferences":[{"database":"PubMed","id":"22159605"},{"database":"DOI","id":"10.1007/s00253-011-3713-5"}],"title":"The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential.","publicationDate":"2012","journal":"Appl. 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