{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H3HZ79","uniProtkbId":"SAP_BURP2","entryAudit":{"firstPublicDate":"2026-01-28","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-09-16","entryVersion":21,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Burkholderia pseudomallei (strain 1026b)","taxonId":884204,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AFI70223.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000010087"}],"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Betaproteobacteria","Burkholderiales","Burkholderiaceae","Burkholderia","pseudomallei group"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Surface attachment protein BP1026B_II1992"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Virulence factor BP1026B_II1992"}}],"flag":"Precursor"},"genes":[{"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AFI70223.1"}],"value":"BP1026B_II1992"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33772012"}],"value":"Required for infection of the mammalian host cells and complete B.pseudomallei pathogenesis in vivo. Attaches to host cells"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33772012"}],"value":"Even distribution throughout the entire cell length"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33772012"}],"value":"Cell surface","id":"SL-0310"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33772012"}],"value":"Reduced cell fusion, i.e. multinucleated giant cell (MNGC) formation, of deletion mutant B.pseudomallei cells-infected RAW264.7 murine macrophages. Deletion of this gene results in dramatically decreased attachment to RAW264.7 macrophages and defects in intracellular replication of the bacterium within them. Deletion mutant cells have also a significant reduction in attachment efficiency to human embryonic kidney HEK293T cells and human neuroblastoma HTB11 cells. Reduced plaque size in deletion mutant-infected HEK293T cells. Attenuated phenotype in BALB/c mouse model of melioidosis. 80% survival of the deletion mutant-infected mice when infected via the intranasal route. Bacterial burdens of the infected mice suggest complete clearance of the mutant in all surviving mice"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Signal","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":20,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":21,"modifier":"EXACT"},"end":{"value":395,"modifier":"EXACT"}},"description":"Surface attachment protein BP1026B_II1992","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_5002611847"}],"keywords":[{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0732","category":"Domain","name":"Signal"},{"id":"KW-0843","category":"Biological process","name":"Virulence"}],"references":[{"referenceNumber":1,"citation":{"id":"22615773","citationType":"journal article","authors":["Hayden H.S.","Lim R.","Brittnacher M.J.","Sims E.H.","Ramage E.R.","Fong C.","Wu Z.","Crist E.","Chang J.","Zhou Y.","Radey M.","Rohmer L.","Haugen E.","Gillett W.","Wuthiekanun V.","Peacock S.J.","Kaul R.","Miller S.I.","Manoil C.","Jacobs M.A."],"citationCrossReferences":[{"database":"PubMed","id":"22615773"},{"database":"DOI","id":"10.1371/journal.pone.0036507"}],"title":"Evolution of Burkholderia pseudomallei in recurrent melioidosis.","publicationDate":"2012","journal":"PLoS ONE","firstPage":"E36507","lastPage":"E36507","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AFI70223.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000010087"}],"value":"1026b","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AFI70223.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000010087"}]},{"referenceNumber":2,"citation":{"id":"33772012","citationType":"journal article","authors":["Heacock-Kang Y.","McMillan I.A.","Norris M.H.","Sun Z.","Zarzycki-Siek J.","Bluhm A.P.","Cabanas D.","Norton R.E.","Ketheesan N.","Miller J.F.","Schweizer H.P.","Hoang T.T."],"citationCrossReferences":[{"database":"PubMed","id":"33772012"},{"database":"DOI","id":"10.1038/s41467-021-22169-1"}],"title":"The Burkholderia pseudomallei intracellular 'TRANSITome'.","publicationDate":"2021","journal":"Nat. Commun.","firstPage":"1907","lastPage":"1907","volume":"12"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33772012"}],"value":"1026b","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP002834","properties":[{"key":"ProteinId","value":"AFI70223.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"A0A0H3HZ79","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A0H3HZ79","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bpz:BP1026B_II1992","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|884204.3.peg.6426","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"Proteomes","id":"UP000010087","properties":[{"key":"Component","value":"Chromosome 2"}]},{"database":"GO","id":"GO:0009986","properties":[{"key":"GoTerm","value":"C:cell surface"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33772012"}]},{"database":"GO","id":"GO:0044228","properties":[{"key":"GoTerm","value":"C:host cell surface"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33772012"}]},{"database":"GO","id":"GO:0046812","properties":[{"key":"GoTerm","value":"F:host cell surface binding"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33772012"}]},{"database":"GO","id":"GO:0141146","properties":[{"key":"GoTerm","value":"P:symbiont-mediated disruption of host tissue"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"33772012"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR021862","properties":[{"key":"EntryName","value":"BPSS1860"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR008964","properties":[{"key":"EntryName","value":"Invasin/intimin_cell_adhesion"}]},{"database":"Pfam","id":"PF11958","properties":[{"key":"EntryName","value":"BPSS1860"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF49373","properties":[{"key":"EntryName","value":"Invasin/intimin cell-adhesion fragments"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKFNCIAATILAAVAADATAAGACLNGSTIASTTRAPLVARQGSVFSSTLYDPAITSNNRTHNPVMLTVQVTNNGRPVAGCDVAWQPRGAGGASGWLFPASASTDANGIASAWWVAGSGAAQTAVASIRRFDGTTQGVAIGGSAQPHATRANSIHLNYEPASDWTAFRVDVTPEALAPTTYWEAIGWPGAYTGIQSIDGKQNGLVLFSVWDVNGKSPQIIAKGPGVDCTQFGGEGTGYKCAKRHAPVAGRTYRFMASIAPVAGQNQTDYSVWFTDTSTNARELIATLRYQKAVQSANYANSFVEDWATQGASCLGATQRAGQYGNVWALDRASAQWRAVKRASTSAVYTPDHNEVCSNYQFSVVNGRFRMSTGGHAVGQPLNLPNGPKSFPLTLP","length":395,"molWeight":41520,"crc64":"0A566450D457E9AD","md5":"FEED1A6360EB13489BC069BA13E8CAF5"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Signal":1,"Chain":1},"uniParcId":"UPI00014F96C4"}}