{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H2ZKY7","uniProtkbId":"ROCS_STRP2","entryAudit":{"firstPublicDate":"2022-12-14","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-09-16","entryVersion":38,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)","taxonId":373153,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ABJ53627.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001452"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Streptococcaceae","Streptococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31182798"}],"value":"Regulator of chromosome segregation"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31182798"}],"value":"rocS"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ABJ53627.1"}],"value":"SPD_0878"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8DQ15"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Required for cell division and chromosome segregation. Binds to DNA and is involved in segregating the origin of replication (oriC) region to new daughter cells (By similarity). When the nucleoid is not properly segregated, involved in blocking the cell division to protect the nucleoid against premature truncation by the newly forming septum, a function which is dependent on CpsD and its autophosphorylation level (PubMed:31182798)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Interacts with CpsD and ParB"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8DQ15"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Localizes at mid-cell at first and then at the future site of division as the cell elongates. Forms one or two bright foci per cell mostly around mid-cell of small cells. Localizes toward the center of the daughter cell as the cell elongates. The bright foci mostly co-localize with origin of replication (oriC). During the cell cycle, very faint, but highly dynamic foci are homogeneously distributed all around the cell periphery (By similarity). Co-localizes with CpsD at mid-cell only at the early stage of the cell cycle (PubMed:31182798)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8DQ15"}],"value":"Cytoplasm, nucleoid","id":"SL-0187"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8DQ15"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8DQ15"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8DQ15"}],"value":"The N-terminal DNA-binding helix-turn-helix (HTH) domain and the C-terminal membrane-binding amphipathic helix (AH) are both required for the function in chromosome segregation and for its proper subcellular location"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Cells lacking this gene have a growth defect with an increased generation time compared to wild-type. Nucleoid defects. 13.9% of the cells are anucleate. No effect on capsule production and polymerization. Deletion of this gene does not impact the cell morphology in a permanently autophosphorylated CpsD mimick mutant. In contrast, the deletion of this gene suppresses the elongated phenotype of the autophosphorylation defective CpsD mimick mutant cells"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Regulator of chromosome segregation","featureId":"PRO_0000456760"}],"keywords":[{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0159","category":"Biological process","name":"Chromosome partition"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"17041037","citationType":"journal article","authors":["Lanie J.A.","Ng W.-L.","Kazmierczak K.M.","Andrzejewski T.M.","Davidsen T.M.","Wayne K.J.","Tettelin H.","Glass J.I.","Winkler M.E."],"citationCrossReferences":[{"database":"PubMed","id":"17041037"},{"database":"DOI","id":"10.1128/jb.01148-06"}],"title":"Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6.","publicationDate":"2007","journal":"J. Bacteriol.","firstPage":"38","lastPage":"51","volume":"189"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001452"}],"value":"D39 / NCTC 7466","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ABJ53627.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001452"}]},{"referenceNumber":2,"citation":{"id":"31182798","citationType":"journal article","authors":["Mercy C.","Ducret A.","Slager J.","Lavergne J.P.","Freton C.","Nagarajan S.N.","Garcia P.S.","Noirot-Gros M.F.","Dubarry N.","Nourikyan J.","Veening J.W.","Grangeasse C."],"citationCrossReferences":[{"database":"PubMed","id":"31182798"},{"database":"DOI","id":"10.1038/s41564-019-0472-z"}],"title":"RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae.","publicationDate":"2019","journal":"Nat. Microbiol.","firstPage":"1661","lastPage":"1670","volume":"4"},"referencePositions":["FUNCTION","INTERACTION WITH CPSD AND PARB","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31182798"}],"value":"D39 / NCTC 7466","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000410","properties":[{"key":"ProteinId","value":"ABJ53627.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_000021242.1","properties":[{"key":"NucleotideSequenceId","value":"NC_008533.2"}]},{"database":"AlphaFoldDB","id":"A0A0H2ZKY7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A0H2ZKY7","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"45218287","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93739471","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spd:SPD_0878","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG5033BIW","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_1561956_0_0_9","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"SPNE373153:G1G6V-964-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001452","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0043590","properties":[{"key":"GoTerm","value":"C:bacterial nucleoid"},{"key":"GoEvidenceType","value":"IEA:GOC"}]},{"database":"GO","id":"GO:0032153","properties":[{"key":"GoTerm","value":"C:cell division site"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0009295","properties":[{"key":"GoTerm","value":"C:nucleoid"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0036386","properties":[{"key":"GoTerm","value":"P:bacterial nucleoid DNA packaging"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31182798"}]},{"database":"GO","id":"GO:2000245","properties":[{"key":"GoTerm","value":"P:negative regulation of FtsZ-dependent cytokinesis"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"InterPro","id":"IPR007489","properties":[{"key":"EntryName","value":"RocS-like_C"}]},{"database":"Pfam","id":"PF04394","properties":[{"key":"EntryName","value":"DUF536"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13412","properties":[{"key":"EntryName","value":"HTH_24"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVTRSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSEKDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARLFGG","length":163,"molWeight":18971,"crc64":"ACE8EFEEF8D2114D","md5":"E11E7EEFD22131AF9E150F06FA6B1D36"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0000051689"}}