{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H2V760","uniProtkbId":"FES_ECOL6","entryAudit":{"firstPublicDate":"2021-04-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-09-16","entryVersion":35,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)","taxonId":199310,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Iron(III) enterobactin esterase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}],"value":"3.1.1.108"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16076215"}],"value":"Fe-Ent esterase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16076215"}],"value":"fes"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAN79146.1"}],"value":"c0671"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}],"value":"Catalyzes the hydrolysis of ferric enterobactin (Fe-Ent). Is responsible for the release of iron from ferric enterobactin. Also catalyzes the hydrolysis of iron-free enterobactin (Ent). Hydrolyzes ferric monoglucosyl-C-Ent (Fe-MGE) poorly and does not hydrolyze ferric diglucosyl-C-Ent (Fe-DGE) or ferric triglucosyl-C-Ent (Fe-TGE) at all. Also hydrolyzes apo MGE, but catalyzes the hydrolysis of apo DGE very poorly, and does not process apo TGE at all. The catalytic efficiency for processing Fe-Ent is much higher than that for apo Ent, suggesting that Fe-Ent is the physiological substrate"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-enterobactin + 3 H2O + H(+) = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] + 2 N-(2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:30111"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:28199"},{"database":"ChEBI","id":"CHEBI:58154"},{"database":"ChEBI","id":"CHEBI:143010"}],"ecNumber":"3.1.1.108","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:30112"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-enterobactin + H2O = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]3 + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59256"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:28199"},{"database":"ChEBI","id":"CHEBI:143011"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59257"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]3 + H2O + H(+) = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]2 + N-(2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59260"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58154"},{"database":"ChEBI","id":"CHEBI:143011"},{"database":"ChEBI","id":"CHEBI:143012"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59261"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine]2 + H2O + H(+) = Fe(III)-[N-(2,3-dihydroxybenzoyl)-L-serine] + N-(2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59264"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58154"},{"database":"ChEBI","id":"CHEBI:143010"},{"database":"ChEBI","id":"CHEBI:143012"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59265"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"enterobactin + 3 H2O = 3 N-(2,3-dihydroxybenzoyl)-L-serine + 2 H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28018"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58154"},{"database":"ChEBI","id":"CHEBI:77805"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":100.0,"unit":"uM","substrate":"MGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":0.29,"unit":"uM","substrate":"Fe-MGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}],"value":"kcat is 9 min(-1) with Fe-Ent as substrate. kcat is 850 min(-1) with MGE as substrate. kcat is 1.4 min(-1) with Fe-MGE as substrate."}]}}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P13039"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Fes family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"Iron(III) enterobactin esterase","featureId":"PRO_0000452105"}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"12471157","citationType":"journal article","authors":["Welch R.A.","Burland V.","Plunkett G. 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