{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H2V660","uniProtkbId":"IROD_ECOL6","entryAudit":{"firstPublicDate":"2021-04-07","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2015-09-16","entryVersion":35,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)","taxonId":199310,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Iron(III) salmochelin esterase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}],"value":"3.1.1.109"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16076215"}],"value":"iroD"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAN79709.1"}],"value":"c1252"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}],"value":"Catalyzes the hydrolysis of both the apo and Fe3(+)-bound forms of enterobactin (Ent), monoglucosyl-C-Ent (MGE), diglucosyl-C-Ent (DGE) and triglucosyl-C-Ent (TGE). Shows higher catalytic efficiencies on Fe3(+)-bound forms. The initial linear trimer products are, in turn, very good substrates for further hydrolytic cleavage by IroD, leading to complete degradation of the trilactone to DHB-Ser and/or Glc-DHB-Ser monomers. Hydrolyzes MGE and DGE regioselectively. May be the ferric MGE/DGE esterase responsible for cytoplasmic iron release"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-C-5-deoxy-beta-D-glucosyl-enterobactin + H2O = Fe(III)-{di[N-(2,3-dihydroxybenzoyl)-L-seryl]-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine} + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:60392"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:143772"},{"database":"ChEBI","id":"CHEBI:143774"}],"ecNumber":"3.1.1.109","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-{di[N-(2,3-dihydroxybenzoyl)-L-seryl]-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine} + H2O + H(+) = Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine} + N-(2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:60404"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58154"},{"database":"ChEBI","id":"CHEBI:143774"},{"database":"ChEBI","id":"CHEBI:143775"}],"ecNumber":"3.1.1.109","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2} + H2O + H(+) = Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine} + N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:60400"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:143773"},{"database":"ChEBI","id":"CHEBI:143775"},{"database":"ChEBI","id":"CHEBI:143778"}],"ecNumber":"3.1.1.109","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-di(C-5-deoxy-beta-D-glucosyl)-enterobactin + H2O = Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2} + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:60388"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:143771"},{"database":"ChEBI","id":"CHEBI:143773"}],"ecNumber":"3.1.1.109","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-{N-(2,3-dihydroxybenzoyl)-L-seryl-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2} + H2O + H(+) = Fe(III)-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2 + N-(2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:60396"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58154"},{"database":"ChEBI","id":"CHEBI:143773"},{"database":"ChEBI","id":"CHEBI:143776"}],"ecNumber":"3.1.1.109","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(III)-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine]2 + H2O + H(+) = Fe(III)-[N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine] + N-(C-5-[deoxy-beta-D-glucosyl]-2,3-dihydroxybenzoyl)-L-serine","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:60408"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:143776"},{"database":"ChEBI","id":"CHEBI:143777"},{"database":"ChEBI","id":"CHEBI:143778"}],"ecNumber":"3.1.1.109","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":40.0,"unit":"uM","substrate":"Ent","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":0.12,"unit":"uM","substrate":"Fe-Ent","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":60.0,"unit":"uM","substrate":"MGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":0.08,"unit":"uM","substrate":"Fe-MGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":120.0,"unit":"uM","substrate":"DGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":0.12,"unit":"uM","substrate":"Fe-DGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":160.0,"unit":"uM","substrate":"TGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]},{"constant":0.43,"unit":"uM","substrate":"Fe-TGE","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16076215"}],"value":"kcat is 1060 min(-1) with Ent as substrate. kcat is 74 min(-1) with Fe-Ent as substrate. kcat is 3720 min(-1) with MGE as substrate. kcat is 46 min(-1) with Fe-MGE as substrate. kcat is 6500 min(-1) with DGE as substrate. kcat is 32 min(-1) with Fe-DGE as substrate. kcat is 4460 min(-1) with TGE as substrate. kcat is 84 min(-1) with Fe-TGE as substrate."}]}}},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"16076215"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Fes family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":409,"modifier":"EXACT"}},"description":"Iron(III) salmochelin esterase","featureId":"PRO_0000452106"}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"12471157","citationType":"journal article","authors":["Welch R.A.","Burland V.","Plunkett G. III","Redford P.","Roesch P.","Rasko D.","Buckles E.L.","Liou S.-R.","Boutin A.","Hackett J.","Stroud D.","Mayhew G.F.","Rose D.J.","Zhou S.","Schwartz D.C.","Perna N.T.","Mobley H.L.T.","Donnenberg M.S.","Blattner F.R."],"citationCrossReferences":[{"database":"PubMed","id":"12471157"},{"database":"DOI","id":"10.1073/pnas.252529799"}],"title":"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli.","publicationDate":"2002","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"17020","lastPage":"17024","volume":"99"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"CFT073 / ATCC 700928 / UPEC","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"16076215","citationType":"journal article","authors":["Lin H.","Fischbach M.A.","Liu D.R.","Walsh C.T."],"citationCrossReferences":[{"database":"PubMed","id":"16076215"},{"database":"DOI","id":"10.1021/ja0522027"}],"title":"In vitro characterization of salmochelin and enterobactin trilactone hydrolases IroD, IroE, and Fes.","publicationDate":"2005","journal":"J. Am. Chem. Soc.","firstPage":"11075","lastPage":"11084","volume":"127"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"CFT073 / ATCC 700928 / UPEC","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE014075","properties":[{"key":"ProteinId","value":"AAN79709.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_000933673.1","properties":[{"key":"NucleotideSequenceId","value":"NC_004431.1"}]},{"database":"AlphaFoldDB","id":"A0A0H2V660","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"A0A0H2V660","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"199310.c1252","properties":[{"key":"Description","value":"-"}]},{"database":"ESTHER","id":"ecoli-IROD","properties":[{"key":"FamilyName","value":"A85-IroE-IroD-Fes-Yiel"}]},{"database":"KEGG","id":"ecc:c1252","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG2382","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_024314_3_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-20652","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"3.1.1.109","properties":[{"key":"OrganismId","value":"2026"}]},{"database":"Proteomes","id":"UP000001410","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0008849","properties":[{"key":"GoTerm","value":"F:enterochelin esterase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005506","properties":[{"key":"GoTerm","value":"F:iron ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006826","properties":[{"key":"GoTerm","value":"P:iron ion transport"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.60.40.10","properties":[{"key":"EntryName","value":"Immunoglobulins"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR021764","properties":[{"key":"EntryName","value":"Enterochelin_esterase_N"}]},{"database":"InterPro","id":"IPR000801","properties":[{"key":"EntryName","value":"Esterase-like"}]},{"database":"InterPro","id":"IPR013783","properties":[{"key":"EntryName","value":"Ig-like_fold"}]},{"database":"InterPro","id":"IPR014756","properties":[{"key":"EntryName","value":"Ig_E-set"}]},{"database":"InterPro","id":"IPR050583","properties":[{"key":"EntryName","value":"Mycobacterial_A85_antigen"}]},{"database":"PANTHER","id":"PTHR48098","properties":[{"key":"EntryName","value":"ENTEROCHELIN ESTERASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR48098:SF3","properties":[{"key":"EntryName","value":"IRON(III) ENTEROBACTIN ESTERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11806","properties":[{"key":"EntryName","value":"Enterochelin_N"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00756","properties":[{"key":"EntryName","value":"Esterase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF81296","properties":[{"key":"EntryName","value":"E set domains"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPVEGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPASYCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKAPALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDIDISQPLGLVVLPDGETWFDHLGVCAAIDAAINNRRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQRQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSETDTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHDYAWWRGALIDGIGLLQG","length":409,"molWeight":44760,"crc64":"E4847E21702B3E76","md5":"AD72E7E4452D17FB8F897C589A16B68D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":6,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00000DE9D1"}}