{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H2V630","uniProtkbId":"IROB_ECOL6","entryAudit":{"firstPublicDate":"2021-04-07","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2021-04-07","entryVersion":50,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)","taxonId":199310,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Enterobactin C-glucosyltransferase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15598734"}],"value":"Ent C-glucosyltransferase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}],"value":"2.4.1.369"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15598734"}],"value":"iroB"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAN79728.1"}],"value":"c1254"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17163637"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}],"value":"Catalyzes the successive monoglucosylation, diglucosylation and triglucosylation of enterobactin (Ent) (PubMed:15598734, PubMed:24960592). Transfers glucosyl groups from uridine-5'-diphosphoglucose (UDP-Glc) to C5 of one, two or three of the 2,3-dihydroxybenzoyl (DHB) units of Ent to yield monoglucosyl-C-Ent (MGE), diglucosyl-C-Ent (DGE) and triglucosyl-C-Ent (TGE) (PubMed:15598734, PubMed:24960592). Glucosylation decreases the membrane affinity of Ent and increases the iron acquisition rate (PubMed:17163637)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"enterobactin + UDP-alpha-D-glucose = monoglucosyl-enterobactin + UDP","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24448"},{"database":"ChEBI","id":"CHEBI:58223"},{"database":"ChEBI","id":"CHEBI:58885"},{"database":"ChEBI","id":"CHEBI:77805"},{"database":"ChEBI","id":"CHEBI:142958"}],"ecNumber":"2.4.1.369","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:24449"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"monoglucosyl-enterobactin + UDP-alpha-D-glucose = diglucosyl-enterobactin + UDP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59096"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58223"},{"database":"ChEBI","id":"CHEBI:58885"},{"database":"ChEBI","id":"CHEBI:142958"},{"database":"ChEBI","id":"CHEBI:142959"}],"ecNumber":"2.4.1.369","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59097"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}]}]},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"diglucosyl-enterobactin + UDP-alpha-D-glucose = triglucosyl-enterobactin + UDP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:59100"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58223"},{"database":"ChEBI","id":"CHEBI:58885"},{"database":"ChEBI","id":"CHEBI:142959"},{"database":"ChEBI","id":"CHEBI:142960"}],"ecNumber":"2.4.1.369","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:59101"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}]}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":5.2,"unit":"uM","substrate":"enterobactin","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"}]},{"constant":13.3,"unit":"uM","substrate":"UDP-Glc (for the first C-glucosylation)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"}],"value":"kcat is 11.2 min(-1) with enterobactin as substrate. kcat is 9.7 min(-1) with UDP-Glc as substrate (for the first C-glucosylation)."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15598734"}],"value":"Siderophore biosynthesis; enterobactin biosynthesis"}],"commentType":"PATHWAY"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24960592"}],"value":"May associate with the inner membrane via hydrophobic and electrostatic interactions"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"24960592"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the glycosyltransferase 28 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAN79728.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":371,"modifier":"EXACT"}},"description":"Enterobactin C-glucosyltransferase","featureId":"PRO_0000452107"},{"type":"Mutagenesis","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Converts 92% of Ent to MGE (60%) and DGE (32%).","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}],"alternativeSequence":{"originalSequence":"HH","alternativeSequences":["AA"]}},{"type":"Mutagenesis","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Converts 29% of Ent to MGE.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"Convert 55% of Ent to MGE (45%) and DGE (10%).","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}],"alternativeSequence":{"originalSequence":"W","alternativeSequences":["L"]}},{"type":"Mutagenesis","location":{"start":{"value":304,"modifier":"EXACT"},"end":{"value":304,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24960592"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0259","category":"Biological process","name":"Enterobactin biosynthesis"},{"id":"KW-0328","category":"Molecular function","name":"Glycosyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"12471157","citationType":"journal article","authors":["Welch R.A.","Burland V.","Plunkett G. 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