{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"A0A0H2URB1","uniProtkbId":"GLYD_STRPN","entryAudit":{"firstPublicDate":"2019-05-08","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2015-09-16","entryVersion":36,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)","taxonId":170187,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Streptococcaceae","Streptococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28246170"}],"value":"Glycosyltransferase GlyD"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"PsrP glycosyltransferase GlyD"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28246170"}],"value":"glyD"},"orderedLocusNames":[{"value":"SP_1767"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28246170"}],"value":"Involved in the polymorphic O-glycosylation of the serine-rich repeat protein PsrP. Catalyzes the third step in glycosylation PsrP in this bacteria. Transfers glucose from UDP-glucose to the terminal glucose moiety of already-glycosylated PsrP (using truncated substrates with PsrP SSR1-GlcNAc-Glc); the C-terminal GT-D domain is sufficient for this reaction in vitro. Also transfers galactose from UDP-galactose to the terminal glucose moiety of already-glycosylated PsrP; the C-terminal GT-D domain is also sufficient for this reaction in vitro. Activity is much higher with UDP-glucose, and the enzyme has a very marked preference for PsrP substrate that has already been modified by GlcNAc and glucose. In vitro has hydrolytic activity against UDP-galactose and to a lesser extent against UDP-glucose"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28246170"}],"value":"Also catalyzes the fourth step in glycosylation of PsrP in this bacteria. Can transfer the sugar from both UDP-glucose and UDP-galactose to the terminal sugar moiety of PsrP-GlcNAc-Glc-Glc and PsrP-GlcNAc-Glc-Gal; the C-terminal GT-D domain is also sufficient for this reaction in vitro (using truncated substrates with glycosylated PsrP SSR1). The N-terminal GT-D domain can transfer galactose from UDP-galactose to PsrP-GlcNAc-Glc-Gal or PsrP-GlcNAc-Glc-Glc in the fourth step"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28246170"}],"value":"Protein modification; protein glycosylation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28246170"}],"value":"Has an N-terminal GT8 domain and a C-terminal GT-D domain. The GT8 has very little glucose transferase activity in the third glycosylation step, but is able to transfer galactose from UDP-galactose to PsrP-GlcNAc-Glc-Gal or PsrP-GlcNAc-Glc-Glc in the fourth step. The C-terminal GT-D domain catalyzes transfer of galactose and glucose to the acceptor protein (PsrP-GlcNAc-Glc) in both the third and fourth glycosylation steps"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11463916"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16861665"},{"evidenceCode":"ECO:0000305"}],"value":"Encoded in RD10, a pathogenicity island with an atypical GC content that is associated with invasive pneumococcal disease. Pathogenicity islands account for greater than half the genomic diversity observed between isolates (PubMed:11463916, PubMed:16861665). The main function of this island seems to be correct synthesis and export of pneumococcal serine-rich repeat protein PsrP (Probable)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28246170"}],"value":"In the N-terminal section; belongs to the glycosyltransferase 8 family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"In the C-terminal section; belongs to the GT-D family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":814,"modifier":"EXACT"}},"description":"Glycosyltransferase GlyD","featureId":"PRO_0000447027"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"GT8 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