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Neutralized by coexpression with cognate antitoxin MntA, which is due to di-AMPylation of the RNase"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(tRNA)-3'-end (ribonucleoside 5'-phosphate)-(guanosyl 5'-phosphate)-(cytosyl 5'-phosphate)-(cytosyl 5'-phosphate)-(adenosine 5'-phosphate) + H2O = GpCpCpA + tRNA 3'-end (ribonucleotide 3'-phosphate) + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:66608"},{"database":"Rhea","id":"RHEA-COMP:17075"},{"database":"Rhea","id":"RHEA-COMP:17076"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:83062"},{"database":"ChEBI","id":"CHEBI:167197"},{"database":"ChEBI","id":"CHEBI:167198"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33290744"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33290744"}],"value":"Homodimer. 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Probably one subunit of the dimer is di-AMPylated, the complex dissociates and then reassociates to di-AMPylate the other HepT subunit"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25593252"}],"value":"Part of a locus that includes subtype I-D CRISPR-Cas genes"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the HepT RNase toxin family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"KHG38990.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33290744"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"tRNA nuclease 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