ID MNME_RICPR Reviewed; 445 AA. AC Q9ZCI1; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1999, sequence version 1. DT 04-NOV-2026, entry version 130. DE RecName: Full=tRNA modification GTPase MnmE {ECO:0000255|HAMAP-Rule:MF_00379}; DE EC=3.6.-.- {ECO:0000255|HAMAP-Rule:MF_00379}; GN Name=mnmE {ECO:0000255|HAMAP-Rule:MF_00379}; GN Synonyms=thdF {ECO:0000255|HAMAP-Rule:MF_00379}, trmE GN {ECO:0000255|HAMAP-Rule:MF_00379}; OrderedLocusNames=RP759; OS Rickettsia prowazekii (strain Madrid E). OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; OC Rickettsiales; Rickettsiaceae; Rickettsieae; Rickettsia; typhus group. OX NCBI_TaxID=272947; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Madrid E; RX PubMed=9823893; DOI=10.1038/24094; RA Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., RA Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., RA Kurland C.G.; RT "The genome sequence of Rickettsia prowazekii and the origin of RT mitochondria."; RL Nature 396:133-140(1998). CC -!- FUNCTION: Exhibits a very high intrinsic GTPase hydrolysis rate. CC Involved in the addition of a carboxymethylaminomethyl (cmnm) group at CC the wobble position (U34) of certain tRNAs, forming tRNA- CC cmnm(5)s(2)U34. {ECO:0000255|HAMAP-Rule:MF_00379}. CC -!- COFACTOR: CC Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000255|HAMAP- CC Rule:MF_00379}; CC Note=Binds 1 potassium ion per subunit. {ECO:0000255|HAMAP- CC Rule:MF_00379}; CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits. CC {ECO:0000255|HAMAP-Rule:MF_00379}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00379}. CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like CC GTPase superfamily. TrmE GTPase family. {ECO:0000255|HAMAP- CC Rule:MF_00379}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJ235273; CAA15187.1; -; Genomic_DNA. DR PIR; C71636; C71636. DR RefSeq; WP_004596984.1; NC_000963.1. DR AlphaFoldDB; Q9ZCI1; -. DR SMR; Q9ZCI1; -. DR STRING; 272947.gene:17555829; -. DR EnsemblBacteria; CAA15187; CAA15187; CAA15187. DR GeneID; 57569882; -. DR KEGG; rpr:RP759; -. DR PATRIC; fig|272947.5.peg.795; -. DR eggNOG; COG0486; Bacteria. DR HOGENOM; CLU_019624_3_1_5; -. DR OMA; AADCTIF; -. DR OrthoDB; 9805918at2; -. DR Proteomes; UP000002480; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule. DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule. DR GO; GO:0030488; P:tRNA methylation; IEA:TreeGrafter. DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:TreeGrafter. DR CDD; cd04164; trmE; 1. DR CDD; cd14858; TrmE_N; 1. DR FunFam; 3.30.1360.120:FF:000007; tRNA modification GTPase GTPBP3, mitochondrial; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR Gene3D; 3.30.1360.120; Probable tRNA modification gtpase trme, domain 1; 1. DR Gene3D; 1.20.120.430; tRNA modification GTPase MnmE domain 2; 1. DR HAMAP; MF_00379; GTPase_MnmE; 1. DR InterPro; IPR031168; G_TrmE. DR InterPro; IPR006073; GTP-bd. DR InterPro; IPR018948; GTP-bd_TrmE_N. DR InterPro; IPR004520; GTPase_MnmE. DR InterPro; IPR027368; MnmE_dom2. DR InterPro; IPR025867; MnmE_helical. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005225; Small_GTP-bd. DR InterPro; IPR027266; TrmE/GcvT-like. DR NCBIfam; NF003661; mnmE; 1. DR NCBIfam; TIGR00450; mnmE_trmE_thdF; 1. DR NCBIfam; TIGR00231; small_GTP; 1. DR PANTHER; PTHR42714; TRNA MODIFICATION GTPASE GTPBP3; 1. DR PANTHER; PTHR42714:SF2; TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL; 1. DR Pfam; PF01926; MMR_HSR1; 1. DR Pfam; PF12631; MnmE_helical; 1. DR Pfam; PF10396; TrmE_N; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR SUPFAM; SSF116878; TrmE connector domain; 1. DR PROSITE; PS51709; G_TRME; 1. PE 3: Inferred from homology; KW Cytoplasm; GTP-binding; Hydrolase; Magnesium; Metal-binding; KW Nucleotide-binding; Potassium; Reference proteome; tRNA processing. FT CHAIN 1..445 FT /note="tRNA modification GTPase MnmE" FT /id="PRO_0000188910" FT DOMAIN 215..371 FT /note="TrmE-type G" FT BINDING 20 FT /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" FT /ligand_id="ChEBI:CHEBI:57457" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 79 FT /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" FT /ligand_id="ChEBI:CHEBI:57457" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 119 FT /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" FT /ligand_id="ChEBI:CHEBI:57457" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 225..230 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 225 FT /ligand="K(+)" FT /ligand_id="ChEBI:CHEBI:29103" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 229 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 244..250 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 244 FT /ligand="K(+)" FT /ligand_id="ChEBI:CHEBI:29103" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 246 FT /ligand="K(+)" FT /ligand_id="ChEBI:CHEBI:29103" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 249 FT /ligand="K(+)" FT /ligand_id="ChEBI:CHEBI:29103" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 250 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 269..272 FT /ligand="GTP" FT /ligand_id="ChEBI:CHEBI:37565" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" FT BINDING 445 FT /ligand="(6S)-5-formyl-5,6,7,8-tetrahydrofolate" FT /ligand_id="ChEBI:CHEBI:57457" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00379" SQ SEQUENCE 445 AA; 49813 MW; FA158F99E716B4F6 CRC64; METIFAQSSA FGKAGVAVFR ISGPKSLEVL QLLTGRKDFK PRLMYYQQII VPETNELIDN AMVVYFKLPN SFTGEDVVEI HTHGSKAISI MLINTLLNIA DIRLAEAGEF TKRAFLNNKF DLTAAEGIAD LINAETIMQH RQAVRQANGG LKELYNKWRN QLLKIISLLE AYIDFPDEDI PESILNDVNN THKNIVNEIS NYLNDNRRGE LLNNGLKLAI IGPPNTGKSS LLNFLMQRNI AIVSNIAGTT RDIIEGHLDI GGYPIILQDT AGIRTESTDI IEQEGIKRAI NSAKTANIKI VMFDAEKLDS SINNDITGLI DENTIVIINK IDLIEPNKTF AIENRYKCLR VSVKNNIALS NILKNIENIA ENLAGVTETP YITNQRHRHY LKQALSHLID FNLDNDLVLA TEDIRMTVRC IGLITGVINV EEILNEIFKN FCIGK //