ID   13S3_FAGES              Reviewed;         538 AA.
AC   Q9XFM4; Q9M641;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   02-SEP-2026, entry version 86.
DE   RecName: Full=13S globulin seed storage protein 3;
DE   AltName: Full=Legumin-like protein 3;
DE   AltName: Allergen=Fag e 1;
DE   Contains:
DE     RecName: Full=13S globulin seed storage protein 3 acidic chain;
DE   Contains:
DE     RecName: Full=13S globulin seed storage protein 3 basic chain;
DE   Flags: Precursor;
GN   Name=FAGAG1;
OS   Fagopyrum esculentum (Common buckwheat) (Polygonum fagopyrum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX   NCBI_TaxID=3617;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Miyazaki zairai;
RA   Nair A., Ohmoto T., Woo S.H., Adachi T.;
RT   "A molecular-genetic approach for hypoallergenic buckwheat.";
RL   Fagopyrum 16:29-36(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 348-538.
RX   PubMed=12806007; DOI=10.1100/tsw.2002.157;
RA   Nair A., Adachi T.;
RT   "Screening and selection of hypoallergenic buckwheat species.";
RL   ScientificWorldJournal 2:818-826(2002).
CC   -!- FUNCTION: Seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC       {ECO:0000250}.
CC   -!- ALLERGEN: Causes an allergic reaction in human.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; AF152003; AAD32713.1; -; mRNA.
DR   EMBL; AF216801; AAF34635.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9XFM4; -.
DR   SMR; Q9XFM4; -.
DR   Allergome; 698; Fag e 1.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:InterPro.
DR   CDD; cd02243; cupin_11S_legumin_C; 1.
DR   CDD; cd02242; cupin_11S_legumin_N; 1.
DR   FunFam; 2.60.120.10:FF:000073; Glycinin G1; 1.
DR   Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   InterPro; IPR050253; Seed_Storage-Functional.
DR   PANTHER; PTHR31189:SF76; 11S GLOBULIN SUBUNIT BETA-LIKE; 1.
DR   PANTHER; PTHR31189; OS03G0336100 PROTEIN-RELATED; 1.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; RmlC-like cupins; 1.
PE   1: Evidence at protein level;
KW   Allergen; Disulfide bond; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..347
FT                   /note="13S globulin seed storage protein 3 acidic chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032007"
FT   CHAIN           348..538
FT                   /note="13S globulin seed storage protein 3 basic chain"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000032008"
FT   DOMAIN          49..301
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          360..509
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          125..194
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          241..272
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          326..346
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        131..194
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        245..268
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        326..340
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        44..77
FT                   /evidence="ECO:0000250"
FT   DISULFID        120..354
FT                   /note="Interchain (between acidic and basic chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   538 AA;  61164 MW;  41D6BA55220CFDAC CRC64;
     MSTKLILSFS LCLMVLSCSA QLLPWQKGQR SRPHHGHQQF QHQCDIQRLT ASEPSRRVRS
     EAGVTEIWDH DTPEFRCAGF VAVRVVIQPG GLLLPSYSNA PYITFVEQGR GVQGVVVPGC
     PETFQSGSEF EYPRSQRDQR SRQSESGESS RGDQRSRQSE SEESSRGDQR SRQSESEEFS
     RGDQHQKIFR IRDGDVIPSP AGVVQWTHNN GDNDLISITL YDANSFQNQL DENVRNFFLA
     GQSKQSREDR RSQRQTREEG SDRQSRESQD DEALLEANIL SGFEDEILQE IFRNVDQETI
     SKLRGENDQR GFIVQARDLK LRVPEEYEEE LQRERGDRKR GGSGRSNGLE QAFCNLKFRQ
     NVNRPSRADV FNPRAGRINT VDSNNLPILE FIQLSAQHVV LYKNAILGPR WNLNAHSALY
     VTRGEGRVQV VGDEGRSVFD DNVQRGQILV VPQGFAVVLK AGREGLEWVE LKNDDNAITS
     PIAGKTSVLR AIPVEVLANS YDISTKEAFR LKNGRQEVEV FRPFQSRDEK ERERFSIV
//