ID   ECD_DROME               Reviewed;         684 AA.
AC   Q9W032;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   02-SEP-2026, entry version 147.
DE   RecName: Full=Protein ecdysoneless {ECO:0000305|PubMed:15128659};
GN   Name=ecd {ECO:0000312|FlyBase:FBgn0000543}; ORFNames=CG5714;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Altocrustacea; Allotriocarida;
OC   Hexapoda; Insecta; Pterygota; Neoptera; Eumetabola; Endopterygota;
OC   Aparaglossata; Panorpida; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Head;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   AND MUTAGENESIS OF PRO-656.
RX   PubMed=15128659; DOI=10.1242/dev.01143;
RA   Gaziova I., Bonnette P.C., Henrich V.C., Jindra M.;
RT   "Cell-autonomous roles of the ecdysoneless gene in Drosophila development
RT   and oogenesis.";
RL   Development 131:2715-2725(2004).
CC   -!- FUNCTION: Required in both the follicle cells and the germline for
CC       oocyte development. {ECO:0000269|PubMed:15128659}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15128659}.
CC   -!- TISSUE SPECIFICITY: Expressed in the ecdysone-producing larval ring
CC       gland, nervous system, imaginal disks and gonads.
CC       {ECO:0000269|PubMed:15128659}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC       throughout development. {ECO:0000269|PubMed:15128659}.
CC   -!- SIMILARITY: Belongs to the ECD family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE014296; AAF47628.1; -; Genomic_DNA.
DR   EMBL; AY051483; AAK92907.1; -; mRNA.
DR   RefSeq; NP_647707.1; NM_139450.3.
DR   AlphaFoldDB; Q9W032; -.
DR   BioGRID; 63806; 21.
DR   FunCoup; Q9W032; 2719.
DR   IntAct; Q9W032; 17.
DR   STRING; 7227.FBpp0072781; -.
DR   PaxDb; 7227-FBpp0072781; -.
DR   PeptideAtlas; Q9W032; -.
DR   DNASU; 38291; -.
DR   EnsemblMetazoa; FBtr0072903; FBpp0072781; FBgn0000543.
DR   GeneID; 38291; -.
DR   KEGG; dme:Dmel_CG5714; -.
DR   UCSC; CG5714-RA; d. melanogaster.
DR   AGR; FB:FBgn0000543; -.
DR   CTD; 11319; -.
DR   FlyBase; FBgn0000543; ecd.
DR   VEuPathDB; VectorBase:FBgn0000543; -.
DR   eggNOG; KOG2406; Eukaryota.
DR   HOGENOM; CLU_006241_2_0_1; -.
DR   InParanoid; Q9W032; -.
DR   OMA; TKDYIWQ; -.
DR   OrthoDB; 27237at2759; -.
DR   PhylomeDB; Q9W032; -.
DR   BioGRID-ORCS; 38291; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 38291; -.
DR   PRO; PR:Q9W032; -.
DR   Proteomes; UP000000803; Chromosome 3L.
DR   Bgee; FBgn0000543; Expressed in T neuron T4d (Drosophila) in embryonic/larval optic lobe (Drosophila) and 51 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0070990; F:snRNP binding; IPI:FlyBase.
DR   GO; GO:0008362; P:chitin-based embryonic cuticle biosynthetic process; IMP:FlyBase.
DR   GO; GO:0006697; P:ecdysone biosynthetic process; TAS:FlyBase.
DR   GO; GO:0007281; P:germ cell development; IMP:FlyBase.
DR   GO; GO:0007390; P:germ-band shortening; IMP:FlyBase.
DR   GO; GO:0007030; P:Golgi organization; IMP:FlyBase.
DR   GO; GO:0008258; P:head involution; IMP:FlyBase.
DR   GO; GO:0002168; P:instar larval development; IMP:FlyBase.
DR   GO; GO:0002165; P:instar larval or pupal development; TAS:FlyBase.
DR   GO; GO:0048542; P:lymph gland development; IMP:FlyBase.
DR   GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR   GO; GO:0007591; P:molting cycle, chitin-based cuticle; IMP:FlyBase.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:FlyBase.
DR   GO; GO:0016319; P:mushroom body development; IMP:FlyBase.
DR   GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR   GO; GO:0007458; P:progression of morphogenetic furrow involved in compound eye morphogenesis; IMP:FlyBase.
DR   GO; GO:0009608; P:response to symbiont; IMP:FlyBase.
DR   InterPro; IPR010770; Ecd.
DR   PANTHER; PTHR13060:SF0; PROTEIN ECDYSONELESS HOMOLOG; 1.
DR   PANTHER; PTHR13060; SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2; 1.
DR   Pfam; PF07093; SGT1; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Developmental protein; Differentiation; Oogenesis;
KW   Reference proteome.
FT   CHAIN           1..684
FT                   /note="Protein ecdysoneless"
FT                   /id="PRO_0000220846"
FT   REGION          491..528
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          603..624
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        491..501
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         656
FT                   /note="P->S: Temperature-sensitive mutation; disrupts
FT                   production of the steroid hormone ecdysone, causes
FT                   developmental and reproductive defects."
FT                   /evidence="ECO:0000269|PubMed:15128659"
SQ   SEQUENCE   684 AA;  77908 MW;  D59B5C943E352F30 CRC64;
     MSKIPGSNLE FVREEDFVEY YIFPKIPDNV QDEGALRKLM LQIRNEISAI VREKSLERSY
     LWHKDEFQLQ VRLGGAEERL LNEETNPEEE AELGDLPPHF HGVTHYGDNI SDEWFVVYLL
     TEITRARGDC IARVSDSDGE FLLIEAADAL PDWASPETCE QRVYLVGGHL QLLQNSAASS
     QDKPLTMAMA VQRIRMNPTL YRCSQEIQSC IDARLKEYQI AQPHFSIHRQ VLELPHSAAQ
     LLKQKPRLLS SAVRAFCERD SLDIKALRTM RYFPPEATRV RTNVRFTRCL YAMLSHQQYL
     PEKRLGWHLT DPVSEPERYK EQLLGLKLAS GLEILATQAK RVEGQQLEDL PAWRSYLRSL
     LSKGYFRDNI EGSAEYQELL NKAKVYFRGN QERFRTASRA GAEILDLLLH PAEAASEELR
     DEENNLQPSD SDEWLNISAE DLDSMLQDRY GPKKLYKPNG QMNAEEFTKQ LAEFLDRQSN
     YEGIEHRGLE EPELDSDDDE PPPQANGSTG LTAKVKKNPS MRKACQRNSV IQPEEPDSTH
     VRNFLDFVIP EDNWDSTSEM SDYADEDDME SNLNALSGGG SVFPLDRQIQ SYMEQMDREL
     AQTSVGKSFH GKKKTAPQAD EDDFDDIEDF EPININVNTL RNMMDSYQSQ VGGAGPVSNL
     FSAMGVGMSA VEDKEQKDIS ESAV
//