ID   FLOWR_HUMAN             Reviewed;         172 AA.
AC   Q9UGQ2; B7Z3T8; B7Z5E1; F5GXX4; Q5SXD4; Q8NBM6;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   02-SEP-2026, entry version 179.
DE   RecName: Full=Calcium channel flower homolog;
DE   AltName: Full=Calcium channel flower domain-containing protein 1;
GN   Name=CACFD1; Synonyms=C9orf7;
GN   ORFNames=PSEC0107, PSEC0248, UNQ3071/PRO9903;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Lymphoid tissue;
RA   Young J.M., Woodward K.J., Aziz S., Burley M., Kwiatkowski D.J., Povey S.;
RT   "Cloning of a sugar transporter gene, a G-beta subunit like gene and three
RT   novel genes in human chromosome 9q34.";
RL   Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=12975309; DOI=10.1101/gr.1293003;
RA   Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA   Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA   Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA   Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA   Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA   Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA   Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA   Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT   "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT   identify novel human secreted and transmembrane proteins: a bioinformatics
RT   assessment.";
RL   Genome Res. 13:2265-2270(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4), AND
RP   VARIANT MET-58.
RC   TISSUE=Teratocarcinoma, and Thalamus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Teratocarcinoma;
RX   PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA   Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA   Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA   Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA   Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA   Isogai T.;
RT   "Signal sequence and keyword trap in silico for selection of full-length
RT   human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT   libraries.";
RL   DNA Res. 12:117-126(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164053; DOI=10.1038/nature02465;
RA   Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA   Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA   Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA   Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA   Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA   Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA   Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA   Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA   Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA   Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA   Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA   Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA   Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA   Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA   Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA   Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA   Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA   McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA   Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA   Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA   Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA   Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA   West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA   Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA   Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA   Dunham I.;
RT   "DNA sequence and analysis of human chromosome 9.";
RL   Nature 429:369-374(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Pancreas;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=31341286; DOI=10.1038/s41586-019-1429-3;
RA   Madan E., Pelham C.J., Nagane M., Parker T.M., Canas-Marques R., Fazio K.,
RA   Shaik K., Yuan Y., Henriques V., Galzerano A., Yamashita T., Pinto M.A.F.,
RA   Palma A.M., Camacho D., Vieira A., Soldini D., Nakshatri H., Post S.R.,
RA   Rhiner C., Yamashita H., Accardi D., Hansen L.A., Carvalho C.,
RA   Beltran A.L., Kuppusamy P., Gogna R., Moreno E.;
RT   "Flower isoforms promote competitive growth in cancer.";
RL   Nature 572:260-264(2019).
RN   [8]
RP   SYNTHESIS OF 1-25; 51-58; 85-95; 121-141 AND 144-172, FUNCTION, SUBCELLULAR
RP   LOCATION (ISOFORMS 1 AND 2), AND MUTAGENESIS OF 25-TRP--LEU-30 AND
RP   149-TYR--ILE-152.
RX   PubMed=37348560; DOI=10.1016/j.jbc.2023.104945;
RA   Rudd J.C., Maity S., Grunkemeyer J.A., Snyder J.C., Lovas S., Hansen L.A.;
RT   "Membrane structure and internalization dynamics of human Flower isoforms
RT   hFWE3 and hFWE4 indicate a conserved endocytic role for hFWE4.";
RL   J. Biol. Chem. 299:104945-104945(2023).
CC   -!- FUNCTION: Transmembrane protein which mediates synaptic endocytosis and
CC       fitness-based cell culling (PubMed:31341286, PubMed:37348560). In
CC       response to different stimulus strengths, controls two major modes of
CC       synaptic vesicle (SV) retrieval in hippocampal neurons; Clathrin-
CC       mediated endocytosis (CME) in response to mild stimulation and
CC       activity-dependent bulk endocytosis (ADBE) in response to strong
CC       stimulation (By similarity). In cytotoxic T-lymphocytes (CTLs)
CC       facilitates calcium-dependent endocytosis of cytotoxic granules at the
CC       immuno synapse (By similarity). Different isoforms work as fitness
CC       fingerprints in 'loser' and 'winner' cells and thereby mediate win/lose
CC       decisions as part of the cell competition process (PubMed:31341286).
CC       {ECO:0000250|UniProtKB:D4A9I3, ECO:0000250|UniProtKB:Q8BG21,
CC       ECO:0000269|PubMed:31341286, ECO:0000269|PubMed:37348560}.
CC   -!- FUNCTION: [Isoform 1]: Functions with the other flower isoforms to
CC       produce tissue-specific fitness fingerprints that identify unfit or fit
CC       cells during cell selection processes in order to maintain tissue
CC       health (PubMed:31341286). During cell competition, if levels of this
CC       isoform in cells is higher than in the surrounding neighboring cells,
CC       the cells are recognized as 'winner' cells, and do not undergo
CC       elimination via apoptosis (PubMed:31341286).
CC       {ECO:0000269|PubMed:31341286}.
CC   -!- FUNCTION: [Isoform 2]: Functions with the other flower isoforms to
CC       produce tissue-specific fitness fingerprints that identify unfit or fit
CC       cells during cell selection processes in order to maintain tissue
CC       health (PubMed:31341286). During cell competition, if levels of this
CC       isoform in unfit cells is higher than in the surrounding neighboring
CC       cells, the cells are recognized as 'loser' cells, and undergo
CC       elimination via apoptosis to be replaced by the surrounding healthy
CC       'winner' cell population (PubMed:31341286).
CC       {ECO:0000269|PubMed:31341286}.
CC   -!- FUNCTION: [Isoform 3]: Functions with the other flower isoforms to
CC       produce tissue-specific fitness fingerprints that identify unfit or fit
CC       cells during cell selection processes in order to maintain tissue
CC       health (PubMed:31341286). During cell competition, if levels of this
CC       isoform in unfit cells is higher than in the surrounding neighboring
CC       cells, the cells are recognized as 'loser' cells, and undergo
CC       elimination via apoptosis to be replaced by the surrounding healthy
CC       'winner' cell population (PubMed:31341286).
CC       {ECO:0000269|PubMed:31341286}.
CC   -!- FUNCTION: [Isoform 4]: Functions with the other flower isoforms to
CC       produce tissue-specific fitness fingerprints that identify unfit or fit
CC       cells during cell selection processes in order to maintain tissue
CC       health (PubMed:31341286). During cell competition, if levels of this
CC       isoform in cells is higher than in the surrounding neighboring cells,
CC       the cells are recognized as 'winner' cells, and do not undergo
CC       elimination via apoptosis (PubMed:31341286).
CC       {ECO:0000269|PubMed:31341286}.
CC   -!- SUBUNIT: Interacts with adaptor protein complex 2 (AP-2).
CC       {ECO:0000250|UniProtKB:Q8BG21}.
CC   -!- INTERACTION:
CC       Q9UGQ2; Q9UBD0: HSFX2; NbExp=3; IntAct=EBI-8652492, EBI-947253;
CC       Q9UGQ2; Q96B96: LDAF1; NbExp=4; IntAct=EBI-8652492, EBI-7055862;
CC       Q9UGQ2; Q96E29: MTERF3; NbExp=3; IntAct=EBI-8652492, EBI-7825321;
CC       Q9UGQ2; Q9ULP0-2: NDRG4; NbExp=3; IntAct=EBI-8652492, EBI-11978907;
CC       Q9UGQ2; Q9UKF7-2: PITPNC1; NbExp=3; IntAct=EBI-8652492, EBI-14223623;
CC       Q9UGQ2; Q96T60: PNKP; NbExp=3; IntAct=EBI-8652492, EBI-1045072;
CC       Q9UGQ2; Q8N0V3: RBFA; NbExp=3; IntAct=EBI-8652492, EBI-3232108;
CC       Q9UGQ2; Q9Y371: SH3GLB1; NbExp=3; IntAct=EBI-8652492, EBI-2623095;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}. Cytoplasmic vesicle, secretory vesicle, synaptic
CC       vesicle {ECO:0000250|UniProtKB:D4A9I3}. Golgi apparatus
CC       {ECO:0000250|UniProtKB:D4A9I3}. Vesicle {ECO:0000250|UniProtKB:Q8BG21}.
CC       Note=In cytotoxic T-lymphocytes, localizes to intracellular vesicles
CC       that move to the immuno synapse (By similarity). Enriched in synaptic
CC       vesicles at the presynaptic vesicles (By similarity). Detected in the
CC       Golgi apparatus of cultured hippocampal neurons (By similarity).
CC       {ECO:0000250|UniProtKB:D4A9I3, ECO:0000250|UniProtKB:Q8BG21}.
CC   -!- SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane
CC       {ECO:0000269|PubMed:37348560}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:37348560}. Early endosome
CC       {ECO:0000269|PubMed:37348560}. Recycling endosome
CC       {ECO:0000269|PubMed:37348560}.
CC   -!- SUBCELLULAR LOCATION: [Isoform 2]: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:37348560}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:37348560}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1; Synonyms=FWE4 {ECO:0000303|PubMed:31341286};
CC         IsoId=Q9UGQ2-1; Sequence=Displayed;
CC       Name=2; Synonyms=FWE3 {ECO:0000303|PubMed:31341286};
CC         IsoId=Q9UGQ2-2; Sequence=VSP_012997;
CC       Name=3; Synonyms=FWE1 {ECO:0000303|PubMed:31341286};
CC         IsoId=Q9UGQ2-3; Sequence=VSP_012997, VSP_042522;
CC       Name=4; Synonyms=FWE2 {ECO:0000303|PubMed:31341286};
CC         IsoId=Q9UGQ2-4; Sequence=VSP_042522;
CC   -!- TISSUE SPECIFICITY: Detected in skin cells at low levels of expression
CC       (at protein level). {ECO:0000269|PubMed:31341286}.
CC   -!- MISCELLANEOUS: In cancer cells, isoforms 1 and 3 isoforms (usually
CC       expressed in 'winner' cells) are up-regulated in cancer tissue whereas
CC       isoforms 2 and 4 isoform (usually expressed in 'loser' cells) are up-
CC       regulated in tissue adjacent to the cancer, suggesting they might be
CC       important for tumor growth. {ECO:0000269|PubMed:31341286}.
CC   -!- SIMILARITY: Belongs to the calcium channel flower family.
CC       {ECO:0000305}.
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DR   EMBL; AJ011373; CAB66156.1; -; mRNA.
DR   EMBL; AK075548; BAC11691.1; -; mRNA.
DR   EMBL; AY358452; AAQ88817.1; -; mRNA.
DR   EMBL; AK074852; BAC11245.1; -; mRNA.
DR   EMBL; AK075416; BAC11606.1; -; mRNA.
DR   EMBL; AK296343; BAH12324.1; -; mRNA.
DR   EMBL; AK298820; BAH12877.1; -; mRNA.
DR   EMBL; AL593848; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC030558; AAH30558.1; -; mRNA.
DR   CCDS; CCDS48051.1; -. [Q9UGQ2-2]
DR   CCDS; CCDS56591.1; -. [Q9UGQ2-4]
DR   CCDS; CCDS56592.1; -. [Q9UGQ2-3]
DR   CCDS; CCDS6974.1; -. [Q9UGQ2-1]
DR   RefSeq; NP_001129247.1; NM_001135775.4. [Q9UGQ2-2]
DR   RefSeq; NP_001229298.1; NM_001242369.2. [Q9UGQ2-4]
DR   RefSeq; NP_001229299.1; NM_001242370.2. [Q9UGQ2-3]
DR   RefSeq; NP_060056.1; NM_017586.5. [Q9UGQ2-1]
DR   AlphaFoldDB; Q9UGQ2; -.
DR   BioGRID; 116275; 22.
DR   FunCoup; Q9UGQ2; 36.
DR   IntAct; Q9UGQ2; 17.
DR   MINT; Q9UGQ2; -.
DR   STRING; 9606.ENSP00000440832; -.
DR   TCDB; 1.A.55.1.1; the synaptic vesicle-associated ca(2+) channel, flower (flower) family.
DR   iPTMnet; Q9UGQ2; -.
DR   PhosphoSitePlus; Q9UGQ2; -.
DR   SwissPalm; Q9UGQ2; -.
DR   BioMuta; CACFD1; -.
DR   DMDM; 61212251; -.
DR   jPOST; Q9UGQ2; -.
DR   MassIVE; Q9UGQ2; -.
DR   PaxDb; 9606-ENSP00000440832; -.
DR   PeptideAtlas; Q9UGQ2; -.
DR   ProteomicsDB; 84256; -. [Q9UGQ2-1]
DR   ProteomicsDB; 84257; -. [Q9UGQ2-2]
DR   Pumba; Q9UGQ2; -.
DR   Antibodypedia; 3031; 92 antibodies from 16 providers.
DR   DNASU; 11094; -.
DR   Ensembl; ENST00000291722.11; ENSP00000291722.7; ENSG00000160325.16. [Q9UGQ2-2]
DR   Ensembl; ENST00000316948.9; ENSP00000317121.4; ENSG00000160325.16. [Q9UGQ2-1]
DR   Ensembl; ENST00000540581.5; ENSP00000440832.1; ENSG00000160325.16. [Q9UGQ2-4]
DR   Ensembl; ENST00000542192.5; ENSP00000444328.1; ENSG00000160325.16. [Q9UGQ2-3]
DR   Ensembl; ENST00000625979.2; ENSP00000486098.1; ENSG00000280479.2. [Q9UGQ2-2]
DR   Ensembl; ENST00000627083.2; ENSP00000486812.1; ENSG00000280479.2. [Q9UGQ2-3]
DR   Ensembl; ENST00000629431.2; ENSP00000486696.1; ENSG00000280479.2. [Q9UGQ2-1]
DR   Ensembl; ENST00000630997.2; ENSP00000485922.1; ENSG00000280479.2. [Q9UGQ2-4]
DR   GeneID; 11094; -.
DR   KEGG; hsa:11094; -.
DR   MANE-Select; ENST00000316948.9; ENSP00000317121.4; NM_017586.5; NP_060056.1.
DR   UCSC; uc004cec.3; human. [Q9UGQ2-1]
DR   AGR; HGNC:1365; -.
DR   ClinPGx; PA25982; -.
DR   CTD; 11094; -.
DR   DisGeNET; 11094; -.
DR   GeneCards; CACFD1; -.
DR   HGNC; HGNC:1365; CACFD1.
DR   HPA; ENSG00000160325; Tissue enhanced (liver).
DR   MIM; 613104; gene.
DR   OpenTargets; ENSG00000160325; -.
DR   VEuPathDB; HostDB:ENSG00000160325; -.
DR   eggNOG; KOG4085; Eukaryota.
DR   GeneTree; ENSGT00390000000529; -.
DR   HOGENOM; CLU_1197263_0_0_1; -.
DR   InParanoid; Q9UGQ2; -.
DR   OMA; YWQKAAL; -.
DR   OrthoDB; 9934994at2759; -.
DR   PAN-GO; Q9UGQ2; 1 GO annotation based on evolutionary models.
DR   PhylomeDB; Q9UGQ2; -.
DR   PathwayCommons; Q9UGQ2; -.
DR   SignaLink; Q9UGQ2; -.
DR   Agora; ENSG00000160325; -.
DR   BioGRID-ORCS; 11094; 12 hits in 1142 CRISPR screens.
DR   ChiTaRS; CACFD1; human.
DR   GenomeRNAi; 11094; -.
DR   Pharos; Q9UGQ2; Tdark.
DR   PRO; PR:Q9UGQ2; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   RNAct; Q9UGQ2; protein.
DR   Bgee; ENSG00000160325; Expressed in mucosa of transverse colon and 97 other cell types or tissues.
DR   ExpressionAtlas; Q9UGQ2; baseline and differential.
DR   GO; GO:0005769; C:early endosome; EXP:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; EXP:UniProtKB.
DR   GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR   GO; GO:0001772; C:immunological synapse; IEA:Ensembl.
DR   GO; GO:0005886; C:plasma membrane; EXP:UniProtKB.
DR   GO; GO:0055037; C:recycling endosome; EXP:UniProtKB.
DR   GO; GO:0008021; C:synaptic vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0031982; C:vesicle; ISS:UniProtKB.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   InterPro; IPR019365; TVP18/Ca-channel_flower.
DR   PANTHER; PTHR13314; CALCIUM CHANNEL FLOWER HOMOLOG; 1.
DR   PANTHER; PTHR13314:SF2; CALCIUM CHANNEL FLOWER HOMOLOG; 1.
DR   Pfam; PF10233; Cg6151-P; 1.
DR   SMART; SM01077; Cg6151-P; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell membrane; Cytoplasmic vesicle;
KW   Endoplasmic reticulum; Endosome; Golgi apparatus; Membrane;
KW   Proteomics identification; Reference proteome; Synapse; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..172
FT                   /note="Calcium channel flower homolog"
FT                   /id="PRO_0000089671"
FT   TOPO_DOM        1..32
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        54..57
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        79..102
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        124..125
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        126..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        143..172
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   VAR_SEQ         65..107
FT                   /note="IMNAFILLLCEAPFCCQFIEFANTVAEKVDRLRSWQKAVFYCG -> M (in
FT                   isoform 2 and isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:16303743"
FT                   /id="VSP_012997"
FT   VAR_SEQ         144..172
FT                   /note="GDAISYARIQQQRQQADEEKLAETLEGEL -> AQTEAGSFAAQHPREPGPF
FT                   SEGTRQAFATPAVVSGEIRMPAGAMRSPMPGSSSRGSRRMRRSSRRPWRGSCEGLGAPP
FT                   SLSPLLALCGSK (in isoform 3 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_042522"
FT   VARIANT         58
FT                   /note="I -> M (in dbSNP:rs3124765)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_069103"
FT   MUTAGEN         25..30
FT                   /note="WWYRWL->AAAAAA: Reduces internalization; when
FT                   associated with 149-A--A-152."
FT                   /evidence="ECO:0000269|PubMed:37348560"
FT   MUTAGEN         149..152
FT                   /note="YARI->AAAA: Reduces internalization; when associated
FT                   with 25-A--A-30."
FT                   /evidence="ECO:0000269|PubMed:37348560"
FT   CONFLICT        110
FT                   /note="V -> D (in Ref. 3; BAH12324)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   172 AA;  18470 MW;  92085A23BDC48439 CRC64;
     MSSSGGAPGA SASSAPPAQE EGMTWWYRWL CRLSGVLGAV SCAISGLFNC ITIHPLNIAA
     GVWMIMNAFI LLLCEAPFCC QFIEFANTVA EKVDRLRSWQ KAVFYCGMAV VPIVISLTLT
     TLLGNAIAFA TGVLYGLSAL GKKGDAISYA RIQQQRQQAD EEKLAETLEG EL
//