ID   BGAL7_ARATH             Reviewed;         826 AA.
AC   Q9SCV5; Q94AU4;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   04-NOV-2026, entry version 148.
DE   RecName: Full=Beta-galactosidase 7;
DE            Short=Lactase 7;
DE            EC=3.2.1.23;
DE   Flags: Precursor;
GN   Name=BGAL7; OrderedLocusNames=At5g20710; ORFNames=T1M15.110;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 39-826.
RA   Gy I., Kreis M., Lecharny A.;
RT   "The beta-galactosidases are encoding by a multigene family in Arabidopsis
RT   thaliana.";
RL   Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 202-826.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17466346; DOI=10.1016/j.phytochem.2007.03.021;
RA   Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P.,
RA   Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.;
RT   "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase
RT   family 35.";
RL   Phytochemistry 68:1510-1520(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in flowers.
CC       {ECO:0000269|PubMed:17466346}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK76465.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF296832; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED92880.1; -; Genomic_DNA.
DR   EMBL; OZ408687; CAO7053020.1; -; Genomic_DNA.
DR   EMBL; AJ270303; CAB64743.1; -; mRNA.
DR   EMBL; AY045791; AAK76465.1; ALT_INIT; mRNA.
DR   RefSeq; NP_568399.4; NM_122078.5.
DR   AlphaFoldDB; Q9SCV5; -.
DR   SMR; Q9SCV5; -.
DR   FunCoup; Q9SCV5; 1.
DR   STRING; 3702.Q9SCV5; -.
DR   GlyCosmos; Q9SCV5; 9 sites, No reported glycans.
DR   GlyGen; Q9SCV5; 9 sites.
DR   PaxDb; 3702-Q9SCV5; -.
DR   ProteomicsDB; 240855; -.
DR   GeneID; 832194; -.
DR   KEGG; ath:AT5G20710; -.
DR   Araport; AT5G20710; -.
DR   TAIR; AT5G20710; BGAL7.
DR   eggNOG; KOG0496; Eukaryota.
DR   HOGENOM; CLU_007853_4_0_1; -.
DR   InParanoid; Q9SCV5; -.
DR   OMA; ACSEDRV; -.
DR   PhylomeDB; Q9SCV5; -.
DR   BioCyc; ARA:AT5G20710-MONOMER; -.
DR   PRO; PR:Q9SCV5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Proteomes; UP001984100; Chromosome 5.
DR   ExpressionAtlas; Q9SCV5; baseline and differential.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; ISM:TAIR.
DR   GO; GO:0009505; C:plant-type cell wall; IBA:GO_Central.
DR   GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0019388; P:galactose catabolic process; IBA:GO_Central.
DR   GO; GO:0009827; P:plant-type cell wall modification; IBA:GO_Central.
DR   CDD; cd22842; Gal_Rha_Lectin_BGal; 1.
DR   FunFam; 2.60.120.260:FF:000097; Beta-galactosidase; 1.
DR   FunFam; 2.60.120.260:FF:000142; Beta-galactosidase; 1.
DR   FunFam; 3.20.20.80:FF:000267; Beta-galactosidase; 1.
DR   Gene3D; 2.60.120.740; -; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR048913; BetaGal_gal-bd.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR041392; GHD.
DR   InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR   InterPro; IPR019801; Glyco_hydro_35_CS.
DR   InterPro; IPR001944; Glycoside_Hdrlase_35.
DR   InterPro; IPR000922; Lectin_gal-bd_dom.
DR   InterPro; IPR043159; Lectin_gal-bd_sf.
DR   PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR   Pfam; PF21467; BetaGal_gal-bd; 1.
DR   Pfam; PF17834; GHD; 1.
DR   Pfam; PF01301; Glyco_hydro_35; 1.
DR   Pfam; PF02140; SUEL_Lectin; 1.
DR   PRINTS; PR00742; GLHYDRLASE35.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR   PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR   PROSITE; PS50228; SUEL_LECTIN; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..826
FT                   /note="Beta-galactosidase 7"
FT                   /id="PRO_0000293092"
FT   DOMAIN          740..826
FT                   /note="SUEL-type lectin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00260"
FT   ACT_SITE        184
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        253
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        154
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        254
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        351
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        380
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        491
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        665
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        706
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        797
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        801
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        674
FT                   /note="E -> K (in Ref. 5; AAK76465)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   826 AA;  92712 MW;  3EDA7EBA3E5D234B CRC64;
     MKMKHFTRLL SLFFILITSL SLAKSTIVSH DERAITINGK RRILLSGSIH YPRSTADMWP
     DLINKAKDGG LDAIETYVFW NAHEPKRREY DFSGNLDVVR FIKTIQDAGL YSVLRIGPYV
     CAEWNYGGFP VWLHNMPNMK FRTVNPSFMN EMQNFTTKIV KMMKEEKLFA SQGGPIILAQ
     IENEYGNVIS SYGAEGKAYI DWCANMANSL DIGVPWLMCQ QPNAPQPMLE TCNGFYCDQY
     EPTNPSTPKM WTENWTGWFK NWGGKHPYRT AEDLAFSVAR FFQTGGTFQN YYMYHGGTNF
     GRVAGGPYIT TSYDYHAPLD EFGNLNQPKW GHLKQLHTVL KSMEKSLTYG NISRIDLGNS
     IKATIYTTKE GSSCFIGNVN ATADALVNFK GKDYHVPAWS VSVLPDCDKE AYNTAKVNTQ
     TSIMTEDSSK PERLEWTWRP ESAQKMILKG SGDLIAKGLV DQKDVTNDAS DYLWYMTRLH
     LDKKDPLWSR NMTLRVHSNA HVLHAYVNGK YVGNQFVKDG KFDYRFERKV NHLVHGTNHI
     SLLSVSVGLQ NYGPFFESGP TGINGPVSLV GYKGEETIEK DLSQHQWDYK IGLNGYNDKL
     FSIKSVGHQK WANEKLPTGR MLTWYKAKFK APLGKEPVIV DLNGLGKGEA WINGQSIGRY
     WPSFNSSDDG CKDECDYRGA YGSDKCAFMC GKPTQRWYHV PRSFLNASGH NTITLFEEMG
     GNPSMVNFKT VVVGTVCARA HEHNKVELSC HNRPISAVKF ASFGNPLGHC GSFAVGTCQG
     DKDAAKTVAK ECVGKLNCTV NVSSDTFGST LDCGDSPKKL AVELEC
//