ID   PLY19_ARATH             Reviewed;         472 AA.
AC   Q9LFP5; A0MFG0;
DT   16-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   04-NOV-2026, entry version 155.
DE   RecName: Full=Probable pectate lyase 19;
DE            EC=4.2.2.2;
DE   Flags: Precursor;
GN   OrderedLocusNames=At5g15110; ORFNames=F2G14.230;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Eliminative cleavage of (1->4)-alpha-D-galacturonan to give
CC         oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at
CC         their non-reducing ends.; EC=4.2.2.2;
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Note=Binds 1 Ca(2+) ion. Required for its activity. {ECO:0000250};
CC   -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-
CC       gluconate from pectin: step 2/5.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 1 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK28696.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL391146; CAC01830.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED92118.1; -; Genomic_DNA.
DR   EMBL; OZ408687; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ653286; ABK28696.1; ALT_SEQ; mRNA.
DR   PIR; T51456; T51456.
DR   RefSeq; NP_197015.4; NM_121515.6.
DR   AlphaFoldDB; Q9LFP5; -.
DR   SMR; Q9LFP5; -.
DR   FunCoup; Q9LFP5; 107.
DR   STRING; 3702.Q9LFP5; -.
DR   GlyCosmos; Q9LFP5; 3 sites, No reported glycans.
DR   GlyGen; Q9LFP5; 3 sites.
DR   PaxDb; 3702-Q9LFP5; -.
DR   PeptideAtlas; Q9LFP5; -.
DR   ProteomicsDB; 234734; -.
DR   GeneID; 831363; -.
DR   KEGG; ath:AT5G15110; -.
DR   Araport; AT5G15110; -.
DR   TAIR; AT5G15110; -.
DR   eggNOG; ENOG502QQE2; Eukaryota.
DR   HOGENOM; CLU_026608_2_1_1; -.
DR   InParanoid; Q9LFP5; -.
DR   OMA; AETTYKH; -.
DR   PhylomeDB; Q9LFP5; -.
DR   BioCyc; ARA:AT5G15110-MONOMER; -.
DR   UniPathway; UPA00545; UER00824.
DR   PRO; PR:Q9LFP5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   Proteomes; UP001984100; Chromosome 5.
DR   GO; GO:0005576; C:extracellular region; ISM:TAIR.
DR   GO; GO:0030570; F:pectate lyase activity; IBA:GO_Central.
DR   GO; GO:0045490; P:pectin catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.160.20.10; Single-stranded right-handed beta-helix, Pectin lyase-like; 1.
DR   InterPro; IPR018082; AmbAllergen.
DR   InterPro; IPR002022; Pec_lyase.
DR   InterPro; IPR007524; Pec_lyase_N.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR045032; PEL.
DR   PANTHER; PTHR31683; PECTATE LYASE 18-RELATED; 1.
DR   PANTHER; PTHR31683:SF161; PECTATE LYASE 19-RELATED; 1.
DR   Pfam; PF04431; Pec_lyase_N; 1.
DR   Pfam; PF00544; Pectate_lyase_4; 1.
DR   PRINTS; PR00807; AMBALLERGEN.
DR   SMART; SM00656; Amb_all; 1.
DR   SUPFAM; SSF51126; Pectin lyase-like; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Glycoprotein; Lyase; Metal-binding; Reference proteome; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..472
FT                   /note="Probable pectate lyase 19"
FT                   /id="PRO_0000024884"
FT   REGION          64..83
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        72..83
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        348
FT                   /evidence="ECO:0000255"
FT   BINDING         268
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         292
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   BINDING         296
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        25
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        66
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        97
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        237
FT                   /note="I -> V (in Ref. 2; AED92118 and 4; ABK28696)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   472 AA;  53851 MW;  E0D84351D7F57D75 CRC64;
     MEMVRLSKLM FTFCIAVLIP TIRGNISELD EYWSQRADEA REFTLQAYHS DPYEIVDHFH
     ERHYDNSTDV TTPEEDGDAK PEEEEKEFIE MLGSSTNSTR RSLRGKGKGK WSKLKGPCTA
     SNPIDKCWRC RSDWAKRRKK LTRCVRGFGH RTTGGKRGRI YVVTSNLDED MVNPKPGTLR
     HAVIQKEPLW IIFKNDMSIR LNQELLINSH KTIDARGANV HVAHGAGITM QFVKNVIIHG
     LHIHHISESS GGMIRDSVDH FGMRTRADGD GLSIYGSSNI WLDHISMSKC QDGLIDAIVG
     STGITISNSH FTHHNDVMLL GAQNTNEADK HMQVTVAYNH FGKGLVQRMP RIRWGFVHVV
     NNDYTHWELY AIGGSQGPTI LSHGNRFIAP PHKPHYREVT KRDYASEDEW KHWNWRSDKD
     VFMNGAYFRQ SGNPQYKCAH TRQQMIKPKN GLAVSKLTKY AGALDCRVGR RC
//