ID LUXO_VIBCH Reviewed; 455 AA. AC Q9KT84; DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2000, sequence version 1. DT 04-NOV-2026, entry version 145. DE RecName: Full=Regulatory protein LuxO; GN Name=luxO; OrderedLocusNames=VC_1021; OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961). OC Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Vibrionales; OC Vibrionaceae; Vibrio. OX NCBI_TaxID=243277; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 39315 / El Tor Inaba N16961; RX PubMed=10952301; DOI=10.1038/35020000; RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A., RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.; RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio RT cholerae."; RL Nature 406:477-483(2000). RN [2] RP INTERACTION WITH SIGMA-54. RX PubMed=9748465; DOI=10.1128/jb.180.19.5256-5259.1998; RA Klose K.E., Novik V., Mekalanos J.J.; RT "Identification of multiple sigma54-dependent transcriptional activators in RT Vibrio cholerae."; RL J. Bacteriol. 180:5256-5259(1998). CC -!- FUNCTION: Involved in the regulation of different processes depending CC on the cell density. Acts together with sigma-54 to repress, perhaps CC indirectly, some genes. CC -!- MISCELLANEOUS: In strain El Tor C6706, at low cell density, LuxO acts CC indirectly on virulence gene expression by repressing hapR This leads CC to the expression of virulence factors. In strain El Tor N16961, the CC hapR gene is inactive due to a natural frameshift mutation. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE003852; AAF94180.1; -; Genomic_DNA. DR PIR; C82250; C82250. DR RefSeq; WP_001888250.1; NZ_LT906614.1. DR SMR; Q9KT84; -. DR STRING; 243277.VC_1021; -. DR DNASU; 2614291; -. DR EnsemblBacteria; AAF94180; AAF94180; VC_1021. DR GeneID; 88785795; -. DR KEGG; vch:VC_1021; -. DR PATRIC; fig|243277.26.peg.975; -. DR eggNOG; COG2204; Bacteria. DR HOGENOM; CLU_000445_0_6_6; -. DR OMA; AADMVRH; -. DR PHI-base; PHI:708; -. DR PRO; PR:Q9KT84; -. DR CDD; cd00009; AAA; 1. DR CDD; cd17572; REC_NtrC1-like; 1. DR FunFam; 3.40.50.300:FF:000006; DNA-binding transcriptional regulator NtrC; 1. DR FunFam; 1.10.10.60:FF:000343; Sigma-54-dependent Fis family transcriptional regulator; 1. DR FunFam; 1.10.8.60:FF:000120; Sigma-54-dependent Fis family transcriptional regulator; 1. DR FunFam; 3.40.50.2300:FF:000225; Sigma-54-dependent Fis family transcriptional regulator; 1. DR Gene3D; 1.10.8.60; -; 1. DR Gene3D; 3.40.50.2300; -; 1. DR Gene3D; 1.10.10.60; Homeodomain-like; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR058031; AAA_lid_NorR. DR InterPro; IPR011006; CheY-like_sf. DR InterPro; IPR009057; Homeodomain-like_sf. DR InterPro; IPR002197; HTH_Fis. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR053402; QS_regulatory_LuxO. DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver. DR InterPro; IPR002078; Sigma_54_int. DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2. DR InterPro; IPR025944; Sigma_54_int_dom_CS. DR PANTHER; PTHR32071:SF117; PTS-DEPENDENT DIHYDROXYACETONE KINASE OPERON REGULATORY PROTEIN-RELATED; 1. DR PANTHER; PTHR32071; TRANSCRIPTIONAL REGULATORY PROTEIN; 1. DR Pfam; PF25601; AAA_lid_14; 1. DR Pfam; PF02954; HTH_8; 1. DR Pfam; PF00072; Response_reg; 1. DR Pfam; PF00158; Sigma54_activat; 1. DR SMART; SM00382; AAA; 1. DR SMART; SM00448; REC; 1. DR SUPFAM; SSF52172; CheY-like; 1. DR SUPFAM; SSF46689; Homeodomain-like; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR NCBIfam; NF041946; LuxO_transreg_Vib; 1. DR PROSITE; PS52089; HTH_NTRC; 1. DR PROSITE; PS50110; RESPONSE_REGULATORY; 1. DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1. DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1. DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1. DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central. DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central. DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:InterPro. DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IBA:GO_Central. DR Proteomes; UP000000584; Chromosome I. DR AlphaFoldDB; Q9KT84; -. PE 1: Evidence at protein level; KW ATP-binding; DNA-binding; Nucleotide-binding; Phosphoprotein; KW Reference proteome; Repressor; Transcription; Transcription regulation; KW Two-component regulatory system. FT CHAIN 1..455 FT /note="Regulatory protein LuxO" FT /id="PRO_0000081115" FT DOMAIN 1..112 FT /note="Response regulatory" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169" FT DOMAIN 132..361 FT /note="Sigma-54 factor interaction" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193" FT DOMAIN 398..447 FT /note="HTH NtrC-type" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01435" FT DNA_BIND 423..446 FT /note="H-T-H motif" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01435" FT BINDING 160..167 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193" FT BINDING 223..232 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193" FT MOD_RES 47 FT /note="4-aspartylphosphate" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169" SQ SEQUENCE 455 AA; 50839 MW; 0D2D2D02B95EF872 CRC64; MVEDTASVAA LYRSYLTPLD IDINIVGTGR DAIESIGRRE PDLILLDLRL PDMTGMDVLY AVKEKSPDVP IVFMTAHGSI DTAVEAMRHG AQDFLIKPCE ADRLRVTVNN AIRKASKLKN DVDNKNQNYQ GFIGSSQTMQ AVYRTIDSAA SSKASIFITG ESGTGKEVCA EAIHAASKRG DKPFIAINCA AIPKDLIESE LFGHVKGAFT GAATERQGAA EAADGGTLFL DELCEMDLDL QTKLLRFIQT GTFQKVGSSK MKSVDVRFVC ATNRDPWKEV QEGRFREDLY YRLYVIPLHL PPLRARGDDV IEIAYSLLGF MSKEEGKDFV RLSAEVVERF RQYEWPGNVR QLQNVLRNVV VLNEGREITL DMLPPPLNQM SAPINRALPL AHENKVSVHE IFPLWMTEKQ AIEQAIEACD GNIPRAATYL DVSPSTIYRK LQTWNEKVKE KEKER //