ID   CHS1_RUTGR              Reviewed;         393 AA.
AC   Q9FSB9;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   28-JAN-2026, entry version 84.
DE   RecName: Full=Chalcone synthase 1;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 1;
GN   Name=CHS1;
OS   Ruta graveolens (Common rue).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Rutaceae; Rutoideae; Ruta.
OX   NCBI_TaxID=37565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Immature flower;
RX   PubMed=11054106; DOI=10.1046/j.1432-1327.2000.01746.x;
RA   Springob K., Lukacin R., Ernwein C., Groening I., Matern U.;
RT   "Specificities of functionally expressed chalcone and acridone synthases
RT   from Ruta graveolens.";
RL   Eur. J. Biochem. 267:6552-6559(2000).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-4-coumaroyl-CoA + 3 malonyl-CoA + 3 H(+) = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15413, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:85008; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; AJ297789; CAC14059.1; -; mRNA.
DR   AlphaFoldDB; Q9FSB9; -.
DR   SMR; Q9FSB9; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-ARBA.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0030639; P:polyketide biosynthetic process; IEA:TreeGrafter.
DR   CDD; cd00831; CHS_like; 1.
DR   FunFam; 3.40.47.10:FF:000014; Chalcone synthase 1; 1.
DR   FunFam; 3.40.47.10:FF:000025; Chalcone synthase 2; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..393
FT                   /note="Chalcone synthase 1"
FT                   /id="PRO_0000216045"
FT   ACT_SITE        166
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   393 AA;  42890 MW;  BFC5C37A42DE4A44 CRC64;
     MAAVTVEAIR KAQRADGPAA VLAIGTATPA NYVTQADYPD YYFRITKSEH MTELKEKFKR
     MCDKSMIRKR YMHLTEDILK ENPNMCAYMA PSLDARQDIV VVEVPKLGKE AAVKAIKEWG
     QPKSKITHLI FCTTSGVDMP GCDYQLTKLL GLRPSVKRFM MYQQGCFAGG TVLRLAKDLA
     ENNRGARVLV VCSEITAVTF RGPADTHLDS LVGQALFGDG AAAVIVGADP DESIERPLYQ
     LVSAAQTILP DSDGAIDGHL REVGLTFHLL KDVPGLISKN IEKSLKEAFG PIGISDWNSI
     FWIAHPGGPA ILDQVEAKLG LKEEKLRATR QVLSEYGNMS SACVLFILDE MRKNCAEEGR
     ATTGEGLDWG VLFGFGPGLT VETVVLRSVP IKA
//